FastQCFastQC Report
Fri 10 Feb 2017
ERR1633175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656652
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53980.8220488173339912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.5751905118693007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20310.3092962482410775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11750.1789380067372063No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9170.13964778908767506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.13660203578150984No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8310.12655104987116464No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7160.10903796836071467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT250.005494648629.68
TTCGCCG1400.029.07142824
GCCGGCA1700.028.29411915
GGTATCA8400.027.970241
CCGGCAG1750.025.3714316
CTAGCAC451.322368E-424.6666683
AGCTTCG1950.023.71794921
GTATCAA23750.023.5242121
ATCTCGC400.001930310723.12511
GCTTCGC1950.022.76923222
GTCTTAT652.679939E-622.769231
CCGCTCT1800.022.61111328
TGCTCGC2300.022.5217410
GCCGCTC1900.021.42105327
CGGCAGC2100.021.14285917
CGCCGGC2400.020.812514
CTGCTCG3300.020.7424249
CTTCGCC2150.020.65116323
GTGTCGT450.003824086920.55555710
GTCGTGT450.003824086920.55555712
Produced by FastQC (version 0.11.5)