##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656652
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864127422135315	33.0	33.0	33.0	33.0	33.0
2	31.972469435865573	33.0	33.0	33.0	33.0	33.0
3	31.70211009789051	33.0	33.0	33.0	27.0	33.0
4	31.979156387249258	33.0	33.0	33.0	33.0	33.0
5	32.072499588823305	33.0	33.0	33.0	33.0	33.0
6	35.23722458775729	37.0	37.0	37.0	33.0	37.0
7	35.3762967294701	37.0	37.0	37.0	33.0	37.0
8	35.51204138569592	37.0	37.0	37.0	33.0	37.0
9	35.62874551512826	37.0	37.0	37.0	33.0	37.0
10	35.62788965844923	37.0	37.0	37.0	33.0	37.0
11	35.64906525831034	37.0	37.0	37.0	33.0	37.0
12	35.62766274982791	37.0	37.0	37.0	33.0	37.0
13	35.63842796488856	37.0	37.0	37.0	33.0	37.0
14	35.620122987518506	37.0	37.0	37.0	33.0	37.0
15	35.63930361896408	37.0	37.0	37.0	33.0	37.0
16	35.61331116024927	37.0	37.0	37.0	33.0	37.0
17	35.60892375261173	37.0	37.0	37.0	33.0	37.0
18	35.59358990759184	37.0	37.0	37.0	33.0	37.0
19	35.585404445581524	37.0	37.0	37.0	33.0	37.0
20	35.59449906495374	37.0	37.0	37.0	33.0	37.0
21	35.581215011908895	37.0	37.0	37.0	33.0	37.0
22	35.488965235771765	37.0	37.0	37.0	33.0	37.0
23	35.52790062316112	37.0	37.0	37.0	33.0	37.0
24	35.52797219836382	37.0	37.0	37.0	33.0	37.0
25	35.54070497006025	37.0	37.0	37.0	33.0	37.0
26	35.425290717153075	37.0	37.0	37.0	33.0	37.0
27	35.44370077301219	37.0	37.0	37.0	33.0	37.0
28	35.45816810121647	37.0	37.0	37.0	33.0	37.0
29	35.46748658345668	37.0	37.0	37.0	33.0	37.0
30	35.47887313219179	37.0	37.0	37.0	33.0	37.0
31	35.453960393024005	37.0	37.0	37.0	33.0	37.0
32	35.452021771044635	37.0	37.0	37.0	33.0	37.0
33	35.4389646266211	37.0	37.0	37.0	33.0	37.0
34	35.40249477653308	37.0	37.0	37.0	33.0	37.0
35	35.33529936709246	37.0	37.0	37.0	33.0	37.0
36	35.358276530034175	37.0	37.0	37.0	33.0	37.0
37	35.351656585223225	37.0	37.0	37.0	33.0	37.0
38	35.33255818911692	37.0	37.0	37.0	33.0	37.0
39	35.220597820458934	37.0	37.0	37.0	33.0	37.0
40	35.00613414715862	37.0	37.0	37.0	27.0	37.0
41	35.15950457776722	37.0	37.0	37.0	33.0	37.0
42	35.19084842504097	37.0	37.0	37.0	33.0	37.0
43	34.84530314382656	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	25.0
16	23.0
17	53.0
18	27.0
19	26.0
20	48.0
21	118.0
22	302.0
23	698.0
24	1569.0
25	2645.0
26	4388.0
27	6711.0
28	9412.0
29	12661.0
30	16388.0
31	20704.0
32	26334.0
33	35069.0
34	50115.0
35	101095.0
36	368239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05713224051705	20.346545811175478	14.206916296607641	23.389405651699835
2	15.740605373927133	22.380499869032608	39.77799504151362	22.10089971552664
3	17.932024877713005	27.900014010465206	30.779926049109722	23.38803506271206
4	12.151794253272662	18.22563549642733	40.24155869471196	29.381011555588042
5	13.632944086060805	37.10626024134549	35.017634911642695	14.243160760951007
6	27.7652394266674	40.715630196816576	17.683795983260538	13.835334393255483
7	25.527219897297197	33.24637707644232	23.281585984661586	17.9448170415989
8	24.299933602577926	35.4435835115099	20.82107417627602	19.435408709636153
9	26.376985069717296	15.403440482934643	20.162886886813716	38.05668756053435
10	15.10175861795898	28.027783361658837	33.43582293208579	23.43463508829639
11	33.28033722580606	24.376838873558597	24.108660294950752	18.234163605684593
12	23.04386493911539	26.142766640473187	30.188440757052444	20.624927663358978
13	28.427995346088945	22.921730231538167	26.38688376796233	22.263390654410557
14	22.465171810944	21.61236088521774	28.603583024189373	27.318884279648888
15	24.197444003825467	28.20260960143272	25.657578138801068	21.942368255940742
16	22.029019937501143	28.894300177262842	25.59194215505321	23.484737730182808
17	22.137448755200623	27.285380993281066	26.521810639425446	24.055359612092857
18	23.2209754938689	26.260332718091167	28.658406583700348	21.86028520433959
19	25.242898826166677	25.74331609436962	28.697392226019264	20.31639285344444
20	25.05786931281714	25.145739295700004	28.479620864628448	21.316770526854405
21	22.855332809463764	26.85349317446684	28.161644219464797	22.129529796604594
22	22.444612976127384	26.62871658047185	28.37621753988414	22.55045290351663
23	22.44948618141725	26.561253144740288	28.973794338553756	22.015466335288707
24	22.634820270097403	26.62338651218606	28.278753434086852	22.463039783629686
25	22.823200112083722	26.507952461882393	28.95323550373714	21.71561192229674
26	23.02650414527025	26.539780583931822	28.39845153901915	22.03526373177878
27	22.797615784311933	26.61196493728794	28.167583438411825	22.422835839988302
28	22.169581452580665	26.757247369992022	28.5708411761481	22.502330001279216
29	23.321028489976424	26.55653222711573	27.87793229899551	22.24450698391233
30	22.857921699774007	26.65399633291302	28.63815232421435	21.84992964309863
31	22.910308656640048	26.30617130534895	28.36570969097787	22.417810347033132
32	21.585862831454104	26.45784981999598	28.795465482477784	23.160821866072133
33	21.890742737401244	26.402264822158468	29.275933066525344	22.431059373914948
34	22.27009131168412	26.448560272412173	28.53566272546189	22.745685690441817
35	22.386591375644937	27.148169806838325	28.27540310545007	22.189835712066667
36	22.93817729939146	26.551202158829945	28.358856746038995	22.1517637957396
37	23.216711439240267	26.146116969109972	28.057936319389874	22.579235272259886
38	23.010666228078193	26.599477348732663	27.939456515780048	22.4503999074091
39	22.49745679598935	26.290790251152817	28.319414240724157	22.89233871213367
40	22.404104457155388	27.06273642660039	28.403781607304936	22.129377508939285
41	22.62979477714223	26.15906142066117	28.463326084440467	22.74781771775613
42	22.266436407716718	26.866742201348657	28.200325286453097	22.66649610448152
43	22.662384337518198	26.47429688784927	28.06874874362676	22.79457003100577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	48.5
2	64.0
3	291.0
4	518.0
5	518.0
6	614.0
7	710.0
8	683.0
9	656.0
10	900.0
11	1144.0
12	1144.0
13	1808.0
14	2472.0
15	4745.0
16	7018.0
17	7905.0
18	8792.0
19	8792.0
20	7859.0
21	6926.0
22	7300.5
23	7675.0
24	9217.5
25	10760.0
26	10760.0
27	12918.0
28	15076.0
29	18062.5
30	21049.0
31	23498.5
32	25948.0
33	25948.0
34	29183.0
35	32418.0
36	34357.0
37	36296.0
38	38976.5
39	41657.0
40	41657.0
41	43842.0
42	46027.0
43	45913.5
44	45800.0
45	47086.5
46	48373.0
47	48373.0
48	48297.5
49	48222.0
50	49298.0
51	50374.0
52	50601.5
53	50829.0
54	50829.0
55	46107.5
56	41386.0
57	36541.0
58	31696.0
59	28146.0
60	24596.0
61	24596.0
62	21471.5
63	18347.0
64	14587.0
65	10827.0
66	9287.0
67	7747.0
68	7747.0
69	6432.5
70	5118.0
71	4320.0
72	3522.0
73	2875.5
74	2229.0
75	2229.0
76	1598.5
77	968.0
78	758.0
79	548.0
80	420.0
81	292.0
82	292.0
83	259.0
84	226.0
85	190.0
86	154.0
87	128.0
88	102.0
89	102.0
90	73.0
91	44.0
92	27.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	656652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.336315239336706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01229678884891	45.875789759894346
2	7.895399623302366	8.422230464979908
3	2.042145023949125	3.2676147228538004
4	0.9676322601032125	2.064397570424672
5	0.5300452725468127	1.4135330873838472
6	0.3625047458848385	1.1600800441361636
7	0.24710049693272607	0.9225601000140443
8	0.1905280829691893	0.8129652716152944
9	0.14824149830088545	0.7115989756434861
>10	1.2896889171989019	14.156862064160675
>50	0.19984642141754558	7.211039785362367
>100	0.11051012890082729	11.003750029370774
>500	0.0029005283176069107	1.0621941809365962
>1k	8.701584952820732E-4	1.0802944895834914
>5k	2.900528317606911E-4	0.8350894536405107
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5398	0.8220488173339912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3777	0.5751905118693007	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2031	0.3092962482410775	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1175	0.1789380067372063	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	917	0.13964778908767506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.13660203578150984	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	831	0.12655104987116464	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	716	0.10903796836071467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.522876653082607E-4	0.0	0.0
3	0.0	0.0	1.522876653082607E-4	0.0	0.0
4	0.0	0.0	6.091506612330427E-4	0.0	0.0
5	0.0	0.0	7.614383265413035E-4	0.0	0.0
6	0.0	0.0	0.0013705889877743463	0.0	0.0
7	0.0	0.0	0.005025492955172603	0.0	0.0
8	0.0	0.0	0.006852944938871731	0.0	0.0
9	0.0	0.0	0.008375821591954338	0.0012183013224660855	0.0
10	6.091506612330427E-4	0.0	0.00868039692257086	0.0012183013224660855	0.0
11	6.091506612330427E-4	0.0	0.012792163885893899	0.0013705889877743463	0.0
12	6.091506612330427E-4	0.0	0.014315040538976505	0.0013705889877743463	0.0
13	6.091506612330427E-4	0.0	0.01507647886551781	0.0013705889877743463	0.0
14	6.091506612330427E-4	0.0	0.016751643183908677	0.0013705889877743463	0.0
15	6.091506612330427E-4	0.0	0.018731382832916064	0.0013705889877743463	0.0
16	6.091506612330427E-4	0.0	0.01964510882476563	0.001522876653082607	0.0
17	7.614383265413035E-4	0.0	0.020254259485998672	0.0016751643183908675	0.0
18	7.614383265413035E-4	0.0	0.020711122481923454	0.0016751643183908675	0.0
19	7.614383265413035E-4	0.0	0.021015697812539976	0.0016751643183908675	0.0
20	7.614383265413035E-4	0.0	0.02162484847377302	0.001979739649007389	0.0
21	9.137259918495642E-4	0.0	0.02253857446562258	0.003350328636781735	0.0
22	9.137259918495642E-4	0.0	0.022843149796239103	0.003959479298014778	0.0
23	9.137259918495642E-4	0.0	0.023147725126855624	0.004111766963323039	0.0
24	9.137259918495642E-4	0.0	0.023300012792163885	0.005786931281713906	0.0
25	9.137259918495642E-4	0.0	0.023300012792163885	0.005786931281713906	0.0
26	9.137259918495642E-4	0.0	0.023300012792163885	0.0062437942776386885	0.0
27	9.137259918495642E-4	0.0	0.023300012792163885	0.007766670930721295	0.0
28	9.137259918495642E-4	0.0	0.023300012792163885	0.016294780187983894	0.0
29	9.137259918495642E-4	0.0	0.023300012792163885	0.032132697380043006	0.0
30	9.137259918495642E-4	0.0	0.023300012792163885	0.056498723829364716	0.0
31	9.137259918495642E-4	0.0	0.023300012792163885	0.11284515999342118	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTGT	25	0.0054946486	29.6	8
TTCGCCG	140	0.0	29.071428	24
GCCGGCA	170	0.0	28.294119	15
GGTATCA	840	0.0	27.97024	1
CCGGCAG	175	0.0	25.37143	16
CTAGCAC	45	1.322368E-4	24.666668	3
AGCTTCG	195	0.0	23.717949	21
GTATCAA	2375	0.0	23.524212	1
ATCTCGC	40	0.0019303107	23.125	11
GCTTCGC	195	0.0	22.769232	22
GTCTTAT	65	2.679939E-6	22.76923	1
CCGCTCT	180	0.0	22.611113	28
TGCTCGC	230	0.0	22.52174	10
GCCGCTC	190	0.0	21.421053	27
CGGCAGC	210	0.0	21.142859	17
CGCCGGC	240	0.0	20.8125	14
CTGCTCG	330	0.0	20.742424	9
CTTCGCC	215	0.0	20.651163	23
GTGTCGT	45	0.0038240869	20.555557	10
GTCGTGT	45	0.0038240869	20.555557	12
>>END_MODULE