FastQCFastQC Report
Fri 10 Feb 2017
ERR1633174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726000
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170732.3516528925619835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT126791.746418732782369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66950.9221763085399449No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA40710.5607438016528926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.43980716253443525No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29650.40840220385674936No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28640.3944903581267218No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG26450.3643250688705234No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT26350.3629476584022039No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23580.3247933884297521No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC22690.312534435261708No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18780.25867768595041324No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18610.25633608815426995No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.24476584022038567No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17010.234297520661157No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16950.2334710743801653No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16620.22892561983471074No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16530.22768595041322315No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC15660.21570247933884296No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15300.21074380165289255No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14860.2046831955922865No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC14330.19738292011019284No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA14230.1960055096418733No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14030.19325068870523415No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13980.1925619834710744No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13660.18815426997245177No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13640.1878787878787879No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13200.18181818181818182No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13060.17988980716253442No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12730.1753443526170799No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12620.17382920110192837No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12610.17369146005509642No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12460.17162534435261706No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12110.1668044077134986No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12090.1665289256198347No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12010.16542699724517906No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11500.15840220385674933No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11140.1534435261707989No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11100.15289256198347106No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11080.15261707988980716No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11070.1524793388429752No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10990.15137741046831957No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10990.15137741046831957No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10930.15055096418732783No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10580.14573002754820935No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10430.14366391184573005No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10380.14297520661157023No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10240.14104683195592288No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10190.1403581267217631No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9990.13760330578512397No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9990.13760330578512397No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9860.13581267217630855No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9810.13512396694214876No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9780.1347107438016529No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9580.13195592286501379No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9550.1315426997245179No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9520.13112947658402205No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9330.12851239669421488No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.1278236914600551No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9280.1278236914600551No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.1274104683195592No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9230.1271349862258953No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9150.12603305785123967No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9130.12575757575757573No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9120.1256198347107438No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9080.12506887052341598No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9080.12506887052341598No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8750.12052341597796143No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8730.12024793388429751No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8560.11790633608815426No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8510.11721763085399449No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8350.1150137741046832No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8340.11487603305785124No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8240.11349862258953167No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8050.11088154269972453No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8030.11060606060606061No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8020.11046831955922864No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7910.10895316804407713No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG7860.10826446280991736No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7520.10358126721763086No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7420.10220385674931129No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7370.10151515151515152No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7280.10027548209366392No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7280.10027548209366392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTTA303.598184E-430.8333341
GCGAACA250.005495026229.634
CCTGAAC250.005495026229.63
CTCCCAT454.0055857E-628.7777751
TCCCATG454.0055857E-628.7777752
CTTCCCT405.9371618E-527.7535
GGTATCA29600.025.251
CCAAGCA601.3362733E-624.66666836
TTCCCTG451.3225568E-424.66666436
CTAGTGC1150.024.1304369
CTCTTAA400.001930505623.1252
GGCCTGT400.001930505623.12536
GTTATCG400.001930505623.12511
GGGATGG1054.0017767E-1122.90476418
AGTGACA903.8216967E-922.61110923
AGGCACC903.8216967E-922.6111098
ACATGTT903.8216967E-922.61110929
CTATATC502.7007738E-422.24
GTATCAA71900.021.8191931
GGCAGCT6500.021.6307718
Produced by FastQC (version 0.11.5)