FastQCFastQC Report
Fri 10 Feb 2017
ERR1633173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361092
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90732.512656054412726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69151.9150244259080789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39781.1016583031471203No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25530.7070220331660629No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17660.4890720370431912No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17160.4752251503771892No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16580.45916276184462684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.4566703222447465No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14410.39906727371417816No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14200.39325158131445725No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13730.38023550784841537No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10580.29300012185260266No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10480.29023074451940223No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10430.28884605585280204No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA10090.27943017291992067No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9930.2749991691868No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9830.27222979185359963No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.27029122772035935No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9710.26890653905375916No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8960.2481362090547561No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8910.24675152038815593No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8810.24398214305495552No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8770.24287439212167536No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC8640.23927420158851484No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8600.23816645065523467No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8580.2376125751885946No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8320.23041219412227354No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8230.22791975452239316No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8050.22293487532263245No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7920.21933468478947193No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7780.21545755652299137No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7640.21158042825651083No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7520.2082571754566703No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7520.2082571754566703No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7370.2041031094568697No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7280.20161066985698936No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7280.20161066985698936No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7270.2013337321236693No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6640.1838866549245068No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6600.18277890399122662No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6510.18028646439134624No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6500.1800095266580262No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6430.17807096252478594No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6430.17807096252478594No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6340.17557852292490556No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6250.1730860833250252No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6110.16920895505854464No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5980.16560876452538412No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5930.1642240758587839No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5920.16394713812546388No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5880.16283938719218372No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5850.1620085739922236No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5820.16117776079226348No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5720.15840838345906305No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5680.15730063252578289No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5680.15730063252578289No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5670.15702369479246286No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5640.15619288159250275No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5630.1559159438591827No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5620.15563900612586265No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5620.15563900612586265No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5600.15508513065922258No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5570.15425431745926246No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5510.15259269105934223No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5330.1476078118595815No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5330.1476078118595815No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5320.14733087412626145No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5270.14594618545966123No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5270.14594618545966123No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5110.1415151817265406No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5070.14040743079326046No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5020.13902274212666024No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4960.13736111572673998No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4940.1368072402600999No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4900.13569948932681974No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4800.13293011199361934No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4760.1318223610603392No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4740.1312684855936991No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4730.1309915478603791No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4690.12988379692709892No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4640.1284991082604987No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4640.1284991082604987No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.1276682950605386No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4590.12711441959389852No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4580.12683748186057844No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4550.12600666866061833No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4500.12462197999401814No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4400.12185260266081774No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4370.12102178946085762No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4300.11908322532761734No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA4290.1188062875942973No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4290.1188062875942973No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4240.1174215989276971No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4150.11492915932781672No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4120.1140983461278566No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC4030.11160590652797625No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC3990.11049815559469608No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3960.10966734239473597No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3940.10911346692809588No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG3940.10911346692809588No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3930.10883652919477585No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3890.10772877826149568No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3880.10745184052817565No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3820.10579021412825541No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.10551327639493538No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3770.10440552546165519No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3750.10385164999501512No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG3750.10385164999501512No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3650.10108227266181471No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3630.10052839719517463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATTT308.296192E-637.021
AGGAATT308.296192E-637.020
TAACAAC200.001840264737.01
GAATTTC308.296192E-637.022
AAGGAAT308.296192E-637.019
CTAGTGC352.3838056E-531.7142879
TGCGGTA303.594633E-430.8333325
TTCGCTA303.594633E-430.83333226
GTTATGC303.594633E-430.8333321
CTTCTCT453.9982533E-628.77777933
TAGTGCA405.9290225E-527.7510
CGTTATT604.305548E-827.74999810
GGTATCA14950.027.224081
GCGGTAT358.857567E-426.4285726
TATGCGG358.857567E-426.4285723
TACCTTA358.857567E-426.42857231
CTACCTT358.857567E-426.42857230
ATGCGGT358.857567E-426.4285724
ACAACGC451.3207577E-424.6666683
GACCGTT901.4188117E-1024.6666687
Produced by FastQC (version 0.11.5)