FastQCFastQC Report
Fri 10 Feb 2017
ERR1633172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504698
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26090.5169428054004572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.4297619566552671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12330.24430451477913526No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5500.10897606093148773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.10818350776107691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT352.3856737E-531.71428515
GGTATCA5350.028.0093481
GATCTAT358.8624755E-426.428571
GCTCGGA809.749783E-1025.43750211
CGGCGTA451.3217756E-424.66666612
GGGACGA451.3217756E-424.66666618
TCACCGG400.001929694123.12500235
CGTTAGA400.001929694123.1250021
TTCGCCG1005.329639E-1022.19999924
CTGCTCG2100.022.0238119
GCCGGCA1107.4578566E-1121.86363615
CGAACTA855.1662937E-821.76470629
GCGAACT855.1662937E-821.76470628
CATAATA957.1213435E-921.4210532
CCGCTCT1059.786163E-1021.14285928
TGCTCGG1059.786163E-1021.14285910
GTATCAA13150.020.9619771
GTCGCCC806.9442103E-720.812537
AGACGTC450.003822879220.55555524
GCTCCGA909.451833E-820.55555529
Produced by FastQC (version 0.11.5)