FastQCFastQC Report
Fri 10 Feb 2017
ERR1633171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745237
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56030.7518413605336288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.5285566873357067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22210.2980259970989095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.1492142768005346No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9960.13364875871702558No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7800.10466469056152605No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7530.10104168204208863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.026.7852781
TTAACGG951.0913936E-1125.3157935
GTATCAA24700.024.9412981
AACGGCC1002.0008883E-1124.0537
GCCGGCA1800.020.55555715
GCTTCGC1850.020.022
CCGGCAG1950.019.92307716
ATAGAAC951.6741433E-719.4736843
TAACGGC1254.110916E-1019.2436
CCGTCCG1254.110916E-1019.2428
CGCCGTC1254.110916E-1019.2426
GCCGTCC1355.638867E-1119.18518427
GCCGCTC1551.8189894E-1219.09677527
TTCGCCG1650.019.06060624
AGATCCG500.007032618418.4999985
ACGGCTA500.007032618418.49999837
AACGATT500.007032618418.49999822
GTGACAC1850.018.024
CCGCTCT1653.6379788E-1217.93939428
GGCACCG1451.546141E-1017.8620689
Produced by FastQC (version 0.11.5)