##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633171.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745237
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850067830770612	33.0	33.0	33.0	27.0	33.0
2	31.96189936892559	33.0	33.0	33.0	33.0	33.0
3	31.691614882245513	33.0	33.0	33.0	27.0	33.0
4	31.982617610236744	33.0	33.0	33.0	33.0	33.0
5	32.076173083193666	33.0	33.0	33.0	33.0	33.0
6	35.22417163935768	37.0	37.0	37.0	33.0	37.0
7	35.37702100137272	37.0	37.0	37.0	33.0	37.0
8	35.50569550357805	37.0	37.0	37.0	33.0	37.0
9	35.614670232422704	37.0	37.0	37.0	33.0	37.0
10	35.60720415116265	37.0	37.0	37.0	33.0	37.0
11	35.64083506320808	37.0	37.0	37.0	33.0	37.0
12	35.62226647361846	37.0	37.0	37.0	33.0	37.0
13	35.62662616053685	37.0	37.0	37.0	33.0	37.0
14	35.601850149683926	37.0	37.0	37.0	33.0	37.0
15	35.627751976887886	37.0	37.0	37.0	33.0	37.0
16	35.60370727701389	37.0	37.0	37.0	33.0	37.0
17	35.601058455229676	37.0	37.0	37.0	33.0	37.0
18	35.585749231452546	37.0	37.0	37.0	33.0	37.0
19	35.57998462234162	37.0	37.0	37.0	33.0	37.0
20	35.58237312425443	37.0	37.0	37.0	33.0	37.0
21	35.5761603355711	37.0	37.0	37.0	33.0	37.0
22	35.47630216964536	37.0	37.0	37.0	33.0	37.0
23	35.52200440933555	37.0	37.0	37.0	33.0	37.0
24	35.5195702843525	37.0	37.0	37.0	33.0	37.0
25	35.53515995582613	37.0	37.0	37.0	33.0	37.0
26	35.42692324723544	37.0	37.0	37.0	33.0	37.0
27	35.44163400367937	37.0	37.0	37.0	33.0	37.0
28	35.46231869861534	37.0	37.0	37.0	33.0	37.0
29	35.46641672380733	37.0	37.0	37.0	33.0	37.0
30	35.47494018681306	37.0	37.0	37.0	33.0	37.0
31	35.46743384990278	37.0	37.0	37.0	33.0	37.0
32	35.45322628908656	37.0	37.0	37.0	33.0	37.0
33	35.44331266429337	37.0	37.0	37.0	33.0	37.0
34	35.41240303420254	37.0	37.0	37.0	33.0	37.0
35	35.33490822382678	37.0	37.0	37.0	33.0	37.0
36	35.36756226542697	37.0	37.0	37.0	33.0	37.0
37	35.352589847256645	37.0	37.0	37.0	33.0	37.0
38	35.33394074636659	37.0	37.0	37.0	33.0	37.0
39	35.22731426378454	37.0	37.0	37.0	33.0	37.0
40	35.013301808686364	37.0	37.0	37.0	27.0	37.0
41	35.17210229765833	37.0	37.0	37.0	33.0	37.0
42	35.20127019994982	37.0	37.0	37.0	33.0	37.0
43	34.87063980988598	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	27.0
16	53.0
17	49.0
18	29.0
19	34.0
20	45.0
21	118.0
22	368.0
23	841.0
24	1733.0
25	3134.0
26	5001.0
27	7388.0
28	10648.0
29	14232.0
30	18701.0
31	23820.0
32	29941.0
33	39744.0
34	56986.0
35	115339.0
36	417000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88493056571265	20.566343324338433	14.089745946591487	23.458980163357428
2	15.712317021296581	22.19736808558888	39.59760452044115	22.49271037267339
3	17.757035681266498	27.94694842043538	30.708620210751747	23.58739568754638
4	11.786317641233595	17.93496565522109	41.11524253358327	29.16347416996204
5	13.414927063471083	37.208163309121794	35.46576458227383	13.911145045133294
6	27.924405256314433	41.271702827422686	17.415131025432178	13.388760890830703
7	25.73691322357854	33.159384195900095	23.55935091789592	17.544351662625445
8	24.628004245629242	35.155930261111564	21.031832826335783	19.18423266692341
9	26.413208147206863	15.157594161320493	20.671544756902836	37.757652934569805
10	14.824679934034407	28.360910690156288	33.97657389528432	22.837835480524987
11	33.16112860740946	24.365671591721828	24.4922085189007	17.980991281968016
12	22.909356352408697	26.417904639732058	30.6044922621931	20.068246745666144
13	28.320655039940313	22.861317943150972	26.401802379645673	22.416224637263046
14	21.89746349147989	21.654587735176865	29.234995041845746	27.212953731497496
15	23.57250109696647	28.972528202437616	25.633858759025653	21.821111941570265
16	21.636472692579677	29.112617865189193	26.12068375563747	23.13022568659366
17	21.87545706936183	27.52251968165832	27.06789920521928	23.534124043760578
18	23.111171345491435	26.466211419991225	28.92019585715685	21.502421377360488
19	24.824720189684623	26.118536787625953	28.74065565719362	20.316087365495807
20	24.570975407823283	25.977776197370765	28.492949222864674	20.958299171941274
21	22.88882597079855	27.17497923479376	28.395127992839864	21.541066801567823
22	22.18193675300609	26.991681840810372	28.9583045393613	21.868076866822232
23	22.138326465272122	26.665879445062444	29.285314604615714	21.910479485049724
24	22.008837457077412	26.971419830201665	28.785876170936227	22.233866541784693
25	22.459029811992696	26.984033267269336	29.10483510614744	21.452101814590527
26	22.477949967594203	26.982154670259263	28.78936499395494	21.750530368191594
27	22.45124705295094	26.952499674600162	28.697313740461087	21.89893953198781
28	22.04131034825163	26.93102999448498	28.868131882877528	22.159527774385868
29	22.562084276545583	27.059177147672486	28.543268785634634	21.835469790147297
30	22.896340358838867	26.80127261528883	28.796208454491655	21.506178571380648
31	22.383483375087387	26.899764772817235	28.757965586786487	21.958786265308888
32	21.47638939022083	26.735253348934634	29.166426250977878	22.62193100986666
33	21.47504753521363	26.99718344633989	29.580388520698786	21.947380497747695
34	22.251444842379	26.47359162253082	28.87779323892936	22.397170296160816
35	22.01205790909469	27.228116693078846	28.630757732104016	22.12906766572245
36	22.59831436173996	26.94324087505049	28.66215713927247	21.796287623937083
37	22.647292069502722	26.690703762695627	28.59412509040748	22.06787907739417
38	22.555375001509585	26.88003950421141	28.36493625517788	22.19964923910112
39	22.107463800106544	26.634882594396146	28.79567171248878	22.461981893008534
40	22.164224266911063	26.981483742755657	28.909729388100697	21.94456260223258
41	22.12598139920589	26.483923906086254	28.870815592891923	22.519279101815933
42	21.594741001855784	27.2958803709424	28.91066868660574	22.19870994059608
43	22.177642816983052	26.69687629572874	28.799428906508933	22.32605198077927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	85.5
2	109.0
3	374.5
4	640.0
5	640.0
6	793.0
7	946.0
8	853.5
9	761.0
10	1061.5
11	1362.0
12	1362.0
13	2172.0
14	2982.0
15	5217.5
16	7453.0
17	8529.0
18	9605.0
19	9605.0
20	9145.0
21	8685.0
22	9462.0
23	10239.0
24	12820.0
25	15401.0
26	15401.0
27	17969.0
28	20537.0
29	23590.0
30	26643.0
31	29670.0
32	32697.0
33	32697.0
34	36741.0
35	40785.0
36	43157.0
37	45529.0
38	46827.0
39	48125.0
40	48125.0
41	49502.5
42	50880.0
43	51471.5
44	52063.0
45	52139.5
46	52216.0
47	52216.0
48	51848.0
49	51480.0
50	51388.5
51	51297.0
52	50219.5
53	49142.0
54	49142.0
55	46032.0
56	42922.0
57	38265.5
58	33609.0
59	30925.5
60	28242.0
61	28242.0
62	24797.0
63	21352.0
64	17715.5
65	14079.0
66	11932.0
67	9785.0
68	9785.0
69	8011.5
70	6238.0
71	5358.5
72	4479.0
73	3350.5
74	2222.0
75	2222.0
76	1615.0
77	1008.0
78	768.0
79	528.0
80	449.0
81	370.0
82	370.0
83	316.5
84	263.0
85	247.0
86	231.0
87	182.0
88	133.0
89	133.0
90	111.5
91	90.0
92	66.0
93	42.0
94	23.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	745237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.2029755355904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.05186160788145	53.89035931850359
2	7.085563338334626	8.673151193039406
3	1.662956408233661	3.0533364110963404
4	0.7176781032815784	1.7569614159028544
5	0.45902144755633106	1.404673921255071
6	0.316934988856001	1.1638418621595865
7	0.2188025193846049	0.9373955668715063
8	0.17521214981883287	0.8578803935120182
9	0.1295498598851728	0.7135953214672255
>10	0.9872227641918419	12.213166835448376
>50	0.12843084552262843	5.432460001240317
>100	0.06388238708103673	7.346743856121443
>500	0.001996324596144709	0.8085667425280206
>1k	6.654415320482364E-4	0.9872225237850514
>5k	2.218138440160788E-4	0.7606446370692579
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5603	0.7518413605336288	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3939	0.5285566873357067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2221	0.2980259970989095	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.1492142768005346	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	996	0.13364875871702558	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	780	0.10466469056152605	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	753	0.10104168204208863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.341855007199052E-4	0.0	0.0
2	0.0	0.0	1.341855007199052E-4	0.0	0.0
3	0.0	0.0	1.341855007199052E-4	0.0	0.0
4	0.0	0.0	4.0255650215971565E-4	1.341855007199052E-4	0.0
5	0.0	0.0	4.0255650215971565E-4	1.341855007199052E-4	0.0
6	0.0	0.0	0.0010734840057592416	1.341855007199052E-4	0.0
7	0.0	0.0	0.0026837100143981044	1.341855007199052E-4	0.0
8	0.0	0.0	0.003220452017277725	1.341855007199052E-4	0.0
9	0.0	0.0	0.003757194020157346	6.709275035995261E-4	0.0
10	5.367420028796208E-4	0.0	0.003891379520877251	6.709275035995261E-4	0.0
11	5.367420028796208E-4	0.0	0.006843460536715165	6.709275035995261E-4	0.0
12	5.367420028796208E-4	0.0	0.007514388040314692	6.709275035995261E-4	0.0
13	5.367420028796208E-4	0.0	0.008856243047513744	6.709275035995261E-4	0.0
14	5.367420028796208E-4	0.0	0.009124614048953554	6.709275035995261E-4	0.0
15	5.367420028796208E-4	0.0	0.00952717055111327	6.709275035995261E-4	0.0
16	6.709275035995261E-4	0.0	0.010466469056152606	8.051130043194313E-4	0.0
17	6.709275035995261E-4	0.0	0.010734840057592418	8.051130043194313E-4	0.0
18	6.709275035995261E-4	0.0	0.010869025558312323	9.392985050393365E-4	0.0
19	6.709275035995261E-4	0.0	0.011271582060472038	9.392985050393365E-4	0.0
20	6.709275035995261E-4	0.0	0.011674138562631753	0.0010734840057592416	0.0
21	6.709275035995261E-4	0.0	0.01207669506479147	0.0013418550071990522	0.0
22	6.709275035995261E-4	0.0	0.01234506606623128	0.0017444115093587678	0.0
23	6.709275035995261E-4	0.0	0.012479251566951184	0.0028178955151180094	0.0
24	6.709275035995261E-4	0.0	0.012479251566951184	0.0034888230187175356	0.0
25	6.709275035995261E-4	0.0	0.012479251566951184	0.003757194020157346	0.0
26	6.709275035995261E-4	0.0	0.012613437067671089	0.004293936023036966	0.0
27	6.709275035995261E-4	0.0	0.012613437067671089	0.005769976530955924	0.0
28	6.709275035995261E-4	0.0	0.012613437067671089	0.016639002089268246	0.0
29	6.709275035995261E-4	0.0	0.012613437067671089	0.03515660118861517	0.0
30	6.709275035995261E-4	0.0	0.012613437067671089	0.061725330331156396	0.0
31	6.709275035995261E-4	0.0	0.012613437067671089	0.12667111267959053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	815	0.0	26.785278	1
TTAACGG	95	1.0913936E-11	25.31579	35
GTATCAA	2470	0.0	24.941298	1
AACGGCC	100	2.0008883E-11	24.05	37
GCCGGCA	180	0.0	20.555557	15
GCTTCGC	185	0.0	20.0	22
CCGGCAG	195	0.0	19.923077	16
ATAGAAC	95	1.6741433E-7	19.473684	3
TAACGGC	125	4.110916E-10	19.24	36
CCGTCCG	125	4.110916E-10	19.24	28
CGCCGTC	125	4.110916E-10	19.24	26
GCCGTCC	135	5.638867E-11	19.185184	27
GCCGCTC	155	1.8189894E-12	19.096775	27
TTCGCCG	165	0.0	19.060606	24
AGATCCG	50	0.0070326184	18.499998	5
ACGGCTA	50	0.0070326184	18.499998	37
AACGATT	50	0.0070326184	18.499998	22
GTGACAC	185	0.0	18.0	24
CCGCTCT	165	3.6379788E-12	17.939394	28
GGCACCG	145	1.546141E-10	17.862068	9
>>END_MODULE