FastQCFastQC Report
Fri 10 Feb 2017
ERR1633170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903798
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51360.5682685732873939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.39190173025388414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19680.2177477710727397No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12910.1428416526701763No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12040.1332156079123875No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC11150.12336827476936219No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11130.12314698638412566No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT9930.10986968326993421No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.10621842491353156No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9370.10367360848331153No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA9360.10356296429069327No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA9130.10101814786047324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.025.8579581
GTATCAA24750.023.3212131
CCGCTCT1600.023.12528
ATACGCG400.001930869923.1255
CGAGGAT450.003825185420.55555516
TTCGCCG1850.020.024
GCCGGCA1950.019.92307715
TCGATTC759.262181E-619.73333237
CGCTCTC2000.019.42529
GCCGCTC1950.018.9743627
GCTTCGC2050.018.95121822
CGAGTCG902.1511805E-618.521
CCGACGC609.2347304E-418.513
AGTACTA500.00703374818.4999985
TGCTCGC2150.018.06976710
TGCTCCG1451.546141E-1017.86206828
CCGGCAG2200.017.65909216
CGCCGTC953.6046622E-617.52631626
CCGTCCG852.7220734E-517.41176428
AGCTTCG2250.017.26666621
Produced by FastQC (version 0.11.5)