##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903798
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.802965928227326	33.0	33.0	33.0	27.0	33.0
2	31.936668370587235	33.0	33.0	33.0	33.0	33.0
3	31.66766910305179	33.0	33.0	33.0	27.0	33.0
4	31.947418560342022	33.0	33.0	33.0	33.0	33.0
5	32.049680348927524	33.0	33.0	33.0	33.0	33.0
6	35.200579111704165	37.0	37.0	37.0	33.0	37.0
7	35.353572369047065	37.0	37.0	37.0	33.0	37.0
8	35.48052219633148	37.0	37.0	37.0	33.0	37.0
9	35.59702721183273	37.0	37.0	37.0	33.0	37.0
10	35.60395685761642	37.0	37.0	37.0	33.0	37.0
11	35.6277774458452	37.0	37.0	37.0	33.0	37.0
12	35.61920473380113	37.0	37.0	37.0	33.0	37.0
13	35.6174156172065	37.0	37.0	37.0	33.0	37.0
14	35.587902385267505	37.0	37.0	37.0	33.0	37.0
15	35.6117108026351	37.0	37.0	37.0	33.0	37.0
16	35.59106680917639	37.0	37.0	37.0	33.0	37.0
17	35.58360164550043	37.0	37.0	37.0	33.0	37.0
18	35.56778063239795	37.0	37.0	37.0	33.0	37.0
19	35.57104021031248	37.0	37.0	37.0	33.0	37.0
20	35.572850349303714	37.0	37.0	37.0	33.0	37.0
21	35.56181359109004	37.0	37.0	37.0	33.0	37.0
22	35.4727472289162	37.0	37.0	37.0	33.0	37.0
23	35.521762606246085	37.0	37.0	37.0	33.0	37.0
24	35.50531534701338	37.0	37.0	37.0	33.0	37.0
25	35.52934615920814	37.0	37.0	37.0	33.0	37.0
26	35.41342534504391	37.0	37.0	37.0	33.0	37.0
27	35.43263428332437	37.0	37.0	37.0	33.0	37.0
28	35.45497002648822	37.0	37.0	37.0	33.0	37.0
29	35.465360622617	37.0	37.0	37.0	33.0	37.0
30	35.46329157621504	37.0	37.0	37.0	33.0	37.0
31	35.450162536318956	37.0	37.0	37.0	33.0	37.0
32	35.440882807884066	37.0	37.0	37.0	33.0	37.0
33	35.43389562712022	37.0	37.0	37.0	33.0	37.0
34	35.41818857753613	37.0	37.0	37.0	33.0	37.0
35	35.35052744086621	37.0	37.0	37.0	33.0	37.0
36	35.36407582225232	37.0	37.0	37.0	33.0	37.0
37	35.36618691344747	37.0	37.0	37.0	33.0	37.0
38	35.3506115304526	37.0	37.0	37.0	33.0	37.0
39	35.25147101454086	37.0	37.0	37.0	33.0	37.0
40	35.03776507582447	37.0	37.0	37.0	27.0	37.0
41	35.19940075105278	37.0	37.0	37.0	33.0	37.0
42	35.248309909957754	37.0	37.0	37.0	33.0	37.0
43	34.8980170347799	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	35.0
16	43.0
17	52.0
18	36.0
19	42.0
20	62.0
21	183.0
22	440.0
23	1026.0
24	2129.0
25	3839.0
26	6202.0
27	9158.0
28	12875.0
29	17217.0
30	22774.0
31	28689.0
32	36526.0
33	48535.0
34	69269.0
35	140144.0
36	504515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46900081655414	21.017085676224113	13.992617819468508	23.521295687753238
2	15.524597310460967	22.485444756461067	39.88424404568278	22.105713887395193
3	17.654829950940364	27.93168384970978	31.194802378407566	23.21868382094229
4	12.295003972126516	18.182713393922093	40.22834748472557	29.293935149225824
5	13.115541304583548	37.973640127550624	35.62975355112536	13.281065016740467
6	28.13858848990593	40.640165169650736	17.859189774706294	13.362056565737035
7	25.087464234264733	33.38511481547868	24.035901827620773	17.49151912263581
8	23.825677861646074	36.318071073403566	21.174532362319898	18.681718702630455
9	25.647213204720526	15.788373065662903	20.6613645969564	37.90304913266017
10	14.342917333297928	28.724449489819627	34.50881723570975	22.423815941172695
11	32.572322576504924	24.544311892701685	24.82556943033731	18.057796100456073
12	22.097415572948822	26.557593621583585	30.99697056200611	20.34802024346148
13	27.46631437555737	22.993633533156746	27.162485422627626	22.37756666865826
14	21.575949493138953	22.039327371824236	28.717700194069913	27.667022940966895
15	23.498834916651727	29.246136858014733	25.731634723688256	21.52339350164528
16	21.98168174747012	29.428035910679156	25.802557651156565	22.78772469069416
17	21.752095047787225	28.01201153355064	27.016324444178895	23.219568974483238
18	22.07075032252782	27.39705111097834	28.87127433342406	21.660924233069778
19	24.119991414010652	26.857218095193836	28.979484353804725	20.043306136990786
20	24.009789798162863	26.295256240885685	28.955253275621324	20.739700685330128
21	22.094760112325986	27.909223078608274	28.163926010015512	21.83209079905023
22	21.639680548087075	27.773352010073047	28.321372696111297	22.26559474572858
23	21.648864016074388	27.5979809647731	28.862533442207216	21.89062157694529
24	22.543643601778275	27.73993746390233	27.92847516812385	21.787943766195543
25	22.435101648819757	27.26195455179144	28.758085324375582	21.544858475013225
26	22.345479852798967	27.340069351779935	28.320819475148205	21.993631320272893
27	22.20706396783352	27.208181474178968	28.33664159469262	22.248112963294894
28	21.412085443871305	27.609377316612782	28.785635728337528	22.192901511178384
29	22.649087517343478	27.64080026731637	28.049851847426083	21.66026036791407
30	22.053157895901517	27.81019652621493	28.382116357858727	21.75452922002483
31	22.440301925872816	27.36009595064384	28.425378237172467	21.774223886310878
32	21.21978583710077	27.46354827074191	28.710287033164487	22.60637885899283
33	21.635254780382343	27.31639149455963	29.04542829260521	22.002925432452827
34	21.667452240434258	27.290943330257424	28.642904719859967	22.39869970944835
35	22.051055656241772	27.905682464444485	28.142239748262334	21.901022131051405
36	22.41607084768942	27.741929059369465	27.994640395309574	21.84735969763155
37	22.76681293828931	26.954142407927435	27.86983374603617	22.409210907747084
38	22.548733234638714	27.553170066762707	28.14666551596706	21.751431182631517
39	22.02427976162815	27.298245846970232	28.505152700050235	22.172321691351385
40	22.190688627326015	27.438542683210187	28.493092483054845	21.877676206408957
41	21.933219591103324	27.335201007304725	28.651866899462046	22.0797125021299
42	22.0009338369857	27.34526962883299	28.422722776549627	22.231073757631684
43	21.979579507810374	27.084038690061274	28.3734861108345	22.562895691293853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	54.5
2	60.0
3	305.0
4	550.0
5	550.0
6	666.0
7	782.0
8	758.0
9	734.0
10	1072.5
11	1411.0
12	1411.0
13	2082.5
14	2754.0
15	5220.5
16	7687.0
17	8829.0
18	9971.0
19	9971.0
20	9569.0
21	9167.0
22	10594.0
23	12021.0
24	14698.0
25	17375.0
26	17375.0
27	21000.5
28	24626.0
29	30837.5
30	37049.0
31	39260.5
32	41472.0
33	41472.0
34	45979.0
35	50486.0
36	52899.0
37	55312.0
38	59232.5
39	63153.0
40	63153.0
41	66754.0
42	70355.0
43	67080.5
44	63806.0
45	64404.5
46	65003.0
47	65003.0
48	64041.5
49	63080.0
50	64191.0
51	65302.0
52	65228.0
53	65154.0
54	65154.0
55	57168.0
56	49182.0
57	43509.0
58	37836.0
59	33401.0
60	28966.0
61	28966.0
62	25162.5
63	21359.0
64	17452.0
65	13545.0
66	11388.0
67	9231.0
68	9231.0
69	7632.5
70	6034.0
71	5240.5
72	4447.0
73	3557.5
74	2668.0
75	2668.0
76	1977.5
77	1287.0
78	981.5
79	676.0
80	539.5
81	403.0
82	403.0
83	353.5
84	304.0
85	274.5
86	245.0
87	202.0
88	159.0
89	159.0
90	113.5
91	68.0
92	45.5
93	23.0
94	14.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	903798.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.536102618461314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.36091019121451	48.516844732214714
2	7.499227866329128	8.329557766873583
3	1.802039295150268	3.0023471765389487
4	0.7859211708007294	1.7458799516644232
5	0.4649284182163424	1.2910156172150844
6	0.2914478489369962	0.9711526587896899
7	0.2307061313469251	0.8968763569633732
8	0.16114771074175605	0.7159612640387426
9	0.12170393217743511	0.6083065859828664
>10	1.0008783904766223	11.34076371281784
>50	0.1558924145372169	6.113833339315556
>100	0.11812907837775294	12.553520339824209
>500	0.005654041355458764	2.1903981491151336
>1k	0.0012115802904554497	1.1475703594048312
>5k	2.0193004840924158E-4	0.5759719892410059
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5136	0.5682685732873939	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3542	0.39190173025388414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.2177477710727397	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1291	0.1428416526701763	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1204	0.1332156079123875	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1115	0.12336827476936219	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1113	0.12314698638412566	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	993	0.10986968326993421	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	960	0.10621842491353156	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	937	0.10367360848331153	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	936	0.10356296429069327	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	913	0.10101814786047324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.212883852365241E-4	0.0	0.0
4	0.0	0.0	2.212883852365241E-4	1.1064419261826205E-4	0.0
5	0.0	0.0	4.425767704730482E-4	2.212883852365241E-4	0.0
6	0.0	0.0	8.851535409460964E-4	2.212883852365241E-4	0.0
7	0.0	0.0	0.0017703070818921927	2.212883852365241E-4	0.0
8	0.0	0.0	0.0025448164302200272	2.212883852365241E-4	0.0
9	0.0	0.0	0.0033193257785478613	7.745093483278343E-4	0.0
10	0.0	0.0	0.0038725467416391717	7.745093483278343E-4	0.0
11	0.0	0.0	0.006085430594004413	8.851535409460964E-4	0.0
12	0.0	0.0	0.007081228327568771	9.957977335643584E-4	0.0
13	0.0	0.0	0.007523805098041819	9.957977335643584E-4	0.0
14	0.0	0.0	0.008077026061133129	0.0011064419261826205	0.0
15	1.1064419261826205E-4	0.0	0.008630247024224439	0.0012170861188008826	0.0
16	1.1064419261826205E-4	0.0	0.009294112179934012	0.0012170861188008826	0.0
17	1.1064419261826205E-4	0.0	0.009404756372552274	0.0012170861188008826	0.0
18	1.1064419261826205E-4	0.0	0.009515400565170536	0.0013277303114191447	0.0
19	1.1064419261826205E-4	0.0	0.009847333143025322	0.0014383745040374065	0.0
20	1.1064419261826205E-4	0.0	0.010068621528261846	0.0015490186966556686	0.0
21	1.1064419261826205E-4	0.0	0.010179265720880109	0.0018809512745104548	0.0
22	1.1064419261826205E-4	0.0	0.01028990991349837	0.002323528044983503	0.0
23	1.1064419261826205E-4	0.0	0.010400554106116632	0.0025448164302200272	0.0
24	1.1064419261826205E-4	0.0	0.010511198298734894	0.0033193257785478613	0.0
25	1.1064419261826205E-4	0.0	0.010511198298734894	0.003983190934257434	0.0
26	1.1064419261826205E-4	0.0	0.010511198298734894	0.00486834447520353	0.0
27	1.1064419261826205E-4	0.0	0.010511198298734894	0.006085430594004413	0.0
28	2.212883852365241E-4	0.0	0.010511198298734894	0.01405181246251928	0.0
29	2.212883852365241E-4	0.0	0.010511198298734894	0.032971969400242086	0.0
30	2.212883852365241E-4	0.0	0.010511198298734894	0.060301084976952815	0.0
31	2.212883852365241E-4	0.0	0.010511198298734894	0.12226183284317955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	880	0.0	25.857958	1
GTATCAA	2475	0.0	23.321213	1
CCGCTCT	160	0.0	23.125	28
ATACGCG	40	0.0019308699	23.125	5
CGAGGAT	45	0.0038251854	20.555555	16
TTCGCCG	185	0.0	20.0	24
GCCGGCA	195	0.0	19.923077	15
TCGATTC	75	9.262181E-6	19.733332	37
CGCTCTC	200	0.0	19.425	29
GCCGCTC	195	0.0	18.97436	27
GCTTCGC	205	0.0	18.951218	22
CGAGTCG	90	2.1511805E-6	18.5	21
CCGACGC	60	9.2347304E-4	18.5	13
AGTACTA	50	0.007033748	18.499998	5
TGCTCGC	215	0.0	18.069767	10
TGCTCCG	145	1.546141E-10	17.862068	28
CCGGCAG	220	0.0	17.659092	16
CGCCGTC	95	3.6046622E-6	17.526316	26
CCGTCCG	85	2.7220734E-5	17.411764	28
AGCTTCG	225	0.0	17.266666	21
>>END_MODULE