FastQCFastQC Report
Fri 10 Feb 2017
ERR1633169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719963
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49110.6821183866393135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35510.49321979046145425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19410.26959718763325335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.12125623122299341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.027.4074081
GCCGCTC1650.023.54545427
TGCTCGC2000.023.12500210
CCGAATT400.0019304923.12514
GTATCAA22150.022.8013551
GCCGGCA1850.022.015
GTCGCCC1201.0913936E-1121.58333237
TTCGCCG1800.021.58333224
CCGGCAG1950.020.87179616
CTGCTCG3550.020.845079
GTCTAGA656.897552E-519.9230771
CTAACTA656.897552E-519.9230779
CGCTCTC1900.019.47368229
AGCTTCG2000.019.42500121
CGGCAGC2200.019.34090817
GCTTCGC2050.018.95122122
CCGCTCT2000.018.528
GCCGATC500.0070323918.513
AACCTCG500.0070323918.55
CATGCTA2000.017.5754
Produced by FastQC (version 0.11.5)