##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719963
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.762278061511495	33.0	33.0	33.0	27.0	33.0
2	31.903895061273982	33.0	33.0	33.0	33.0	33.0
3	31.630333781041525	33.0	33.0	33.0	27.0	33.0
4	31.921372348301233	33.0	33.0	33.0	33.0	33.0
5	32.02235809340202	33.0	33.0	33.0	33.0	33.0
6	35.15226338020148	37.0	37.0	37.0	33.0	37.0
7	35.31016316116245	37.0	37.0	37.0	33.0	37.0
8	35.43484873528223	37.0	37.0	37.0	33.0	37.0
9	35.54535858092707	37.0	37.0	37.0	33.0	37.0
10	35.56269836088799	37.0	37.0	37.0	33.0	37.0
11	35.58356609992458	37.0	37.0	37.0	33.0	37.0
12	35.56563184496981	37.0	37.0	37.0	33.0	37.0
13	35.569132024840165	37.0	37.0	37.0	33.0	37.0
14	35.55027272234823	37.0	37.0	37.0	33.0	37.0
15	35.56129134413852	37.0	37.0	37.0	33.0	37.0
16	35.54854207785678	37.0	37.0	37.0	33.0	37.0
17	35.53766374105336	37.0	37.0	37.0	33.0	37.0
18	35.52583118854719	37.0	37.0	37.0	33.0	37.0
19	35.516201527022915	37.0	37.0	37.0	33.0	37.0
20	35.514443103326144	37.0	37.0	37.0	33.0	37.0
21	35.51931279801879	37.0	37.0	37.0	33.0	37.0
22	35.41510883198164	37.0	37.0	37.0	33.0	37.0
23	35.46660175592357	37.0	37.0	37.0	33.0	37.0
24	35.45754156810836	37.0	37.0	37.0	33.0	37.0
25	35.48408043191108	37.0	37.0	37.0	33.0	37.0
26	35.367024416532516	37.0	37.0	37.0	33.0	37.0
27	35.383032183598324	37.0	37.0	37.0	33.0	37.0
28	35.401565080427744	37.0	37.0	37.0	33.0	37.0
29	35.40032751683073	37.0	37.0	37.0	33.0	37.0
30	35.40209983013016	37.0	37.0	37.0	33.0	37.0
31	35.386035115693446	37.0	37.0	37.0	33.0	37.0
32	35.379972304132295	37.0	37.0	37.0	33.0	37.0
33	35.37178299440388	37.0	37.0	37.0	33.0	37.0
34	35.34420518832218	37.0	37.0	37.0	33.0	37.0
35	35.27946852824381	37.0	37.0	37.0	33.0	37.0
36	35.300157091406085	37.0	37.0	37.0	33.0	37.0
37	35.2991264828887	37.0	37.0	37.0	33.0	37.0
38	35.28909402288729	37.0	37.0	37.0	33.0	37.0
39	35.180648172197735	37.0	37.0	37.0	33.0	37.0
40	34.95785338968808	37.0	37.0	37.0	27.0	37.0
41	35.125485615233	37.0	37.0	37.0	33.0	37.0
42	35.15961514688949	37.0	37.0	37.0	33.0	37.0
43	34.812350079101286	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	26.0
16	55.0
17	37.0
18	20.0
19	41.0
20	72.0
21	140.0
22	377.0
23	918.0
24	1787.0
25	3306.0
26	5131.0
27	7896.0
28	10527.0
29	14299.0
30	18578.0
31	23664.0
32	30172.0
33	39568.0
34	56771.0
35	113703.0
36	392874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25989807809568	20.97246664064681	14.207118976947427	23.560516304310084
2	15.571216854199452	22.458654125281438	39.76718248021079	22.20294654030832
3	17.617710910143995	27.982687999244405	31.07409686331103	23.32550422730057
4	12.16506959385413	18.2278811550038	40.71667571805773	28.89037353308434
5	13.136369507877488	37.73680036335201	35.57585598148793	13.550974147282568
6	27.86226514418102	41.28976072381497	17.659379718124406	13.188594413879603
7	25.306161566636064	33.19531698156711	24.046374605361663	17.452146846435166
8	24.08443211665044	36.09713276932287	20.988717475759174	18.82971763826752
9	26.065367248039134	15.464128017689799	20.74606611728658	37.724438616984486
10	14.549636578546398	28.56605131097015	34.42593577725522	22.458376333228237
11	32.80071336999262	24.55473406272267	24.63848836676329	18.006064200521415
12	22.235031522453237	26.686371382973846	31.012149235446824	20.066447859126093
13	27.87573805876135	22.8917319362245	26.79734930822834	22.435180696785807
14	21.754867958492312	21.890291584428645	29.083439010060243	27.271401447018807
15	23.34592194321097	29.314561998324912	25.64215105498477	21.697365003479348
16	21.72125512005478	29.363314503661993	26.056339006310047	22.85909136997318
17	21.484992978805856	28.038246409884955	27.365017368948124	23.111743242361065
18	22.55824257635462	27.126949579353383	28.930514484772136	21.384293359519866
19	24.300137645962362	26.527057640462083	29.057743245138983	20.115061468436572
20	24.083320948437628	26.38968947015333	28.808702669442738	20.71828691196631
21	22.316146801988435	27.469050492872547	28.602442069939706	21.61236063519931
22	21.916959621536105	27.374462298757017	28.746199457472123	21.962378622234755
23	21.785008396264807	27.049306700483218	29.364286775848203	21.801398127403772
24	22.091413030947425	27.523219943247085	28.47826902215808	21.90709800364741
25	22.22933678536258	27.134033276710053	29.15219254322792	21.484437394699448
26	22.0282153388438	27.39390774248121	28.856066214513802	21.721810704161186
27	22.090440758761215	27.41113084977978	28.42729418039538	22.071134211063622
28	21.56805280271347	27.408491825274357	29.11732964055097	21.906125731461202
29	22.66602589299728	27.226815822479765	28.453545529423042	21.653612755099914
30	22.23822613106507	27.31876499208987	28.93648701391599	21.506521862929066
31	22.314896737749024	27.108893095895205	28.82675915290091	21.749451013454856
32	21.375404013817377	27.09194778064984	29.068299343160692	22.464348862372095
33	21.372348301232147	27.132783212470642	29.601104501203533	21.893763985093678
34	21.888347040056225	26.858046871853137	29.032741960350737	22.220864127739897
35	21.9566838851441	27.614752424777382	28.70119714485328	21.72736654522524
36	22.285728572162736	27.454327514052807	28.579940913630285	21.680003000154173
37	22.386983775555134	26.908882817589237	28.497714465882275	22.206418940973354
38	22.307813040392354	27.248622498656182	28.522993542723725	21.920570918227742
39	21.889458208269037	27.19556421649446	28.714253371353806	22.2007242038827
40	21.834455381734895	27.443354727951295	28.957877002012605	21.764312888301205
41	21.902931122849367	26.986942384539205	28.901346319185844	22.20878017342558
42	21.660974244509788	27.51891416642244	28.711753242874984	22.10835834619279
43	21.935988377180493	26.940828903707555	28.727865181960738	22.395317537151215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	66.5
2	81.0
3	289.5
4	498.0
5	498.0
6	589.0
7	680.0
8	652.0
9	624.0
10	897.0
11	1170.0
12	1170.0
13	1794.5
14	2419.0
15	4614.5
16	6810.0
17	7770.5
18	8731.0
19	8731.0
20	8291.0
21	7851.0
22	8757.0
23	9663.0
24	11896.5
25	14130.0
26	14130.0
27	17057.5
28	19985.0
29	23885.0
30	27785.0
31	30839.5
32	33894.0
33	33894.0
34	37590.0
35	41286.0
36	43720.5
37	46155.0
38	48018.5
39	49882.0
40	49882.0
41	51625.5
42	53369.0
43	52891.5
44	52414.0
45	52013.5
46	51613.0
47	51613.0
48	49937.0
49	48261.0
50	48979.0
51	49697.0
52	48658.0
53	47619.0
54	47619.0
55	43900.0
56	40181.0
57	35295.5
58	30410.0
59	27243.5
60	24077.0
61	24077.0
62	21047.5
63	18018.0
64	14620.0
65	11222.0
66	9621.0
67	8020.0
68	8020.0
69	6507.0
70	4994.0
71	4246.0
72	3498.0
73	2891.0
74	2284.0
75	2284.0
76	1676.0
77	1068.0
78	806.0
79	544.0
80	448.0
81	352.0
82	352.0
83	293.0
84	234.0
85	205.5
86	177.0
87	144.5
88	112.0
89	112.0
90	90.5
91	69.0
92	47.5
93	26.0
94	16.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	719963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.223812139141664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37226135169296	54.104867273071775
2	6.8513572279168615	8.389324156402646
3	1.5660704357583242	2.8764240646659407
4	0.7263995479305724	1.7789179784183515
5	0.4431962020869811	1.3567080508677194
6	0.28583346627251915	1.0499888665289037
7	0.2059066660380907	0.8824473727799131
8	0.17155799996930893	0.8402747808870271
9	0.1339401246498651	0.7380292526510903
>10	1.0431965936490495	13.151998301189064
>50	0.1298497219658712	5.457192542476936
>100	0.06813650369610691	7.342536472117585
>500	0.0016059108614823526	0.5701154552732163
>1k	6.882475120638655E-4	1.4611754326699355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4911	0.6821183866393135	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3551	0.49321979046145425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.26959718763325335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.12125623122299341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.1668807980410106E-4	0.0	0.0
2	0.0	0.0	4.1668807980410106E-4	0.0	0.0
3	0.0	0.0	6.944801330068351E-4	0.0	0.0
4	0.0	0.0	8.333761596082021E-4	1.38896026601367E-4	0.0
5	1.38896026601367E-4	0.0	9.722721862095691E-4	1.38896026601367E-4	0.0
6	1.38896026601367E-4	0.0	0.0015278562926150372	1.38896026601367E-4	0.0
7	1.38896026601367E-4	0.0	0.0033335046384328085	5.55584106405468E-4	0.0
8	1.38896026601367E-4	0.0	0.004027984771439643	5.55584106405468E-4	0.0
9	2.77792053202734E-4	0.0	0.005000256957649212	0.001805648345817771	0.0
10	2.77792053202734E-4	0.0	0.00513915298425058	0.001805648345817771	0.0
11	2.77792053202734E-4	1.38896026601367E-4	0.00791707351627792	0.002083440399020505	0.0
12	2.77792053202734E-4	1.38896026601367E-4	0.009028241729088856	0.002222336425621872	0.0
13	2.77792053202734E-4	1.38896026601367E-4	0.009722721862095692	0.002222336425621872	0.0
14	2.77792053202734E-4	1.38896026601367E-4	0.01069499404830526	0.002222336425621872	0.0
15	2.77792053202734E-4	1.38896026601367E-4	0.012222850340920298	0.0023612324522232393	0.0
16	2.77792053202734E-4	1.38896026601367E-4	0.012917330473927133	0.002500128478824606	0.0
17	2.77792053202734E-4	1.38896026601367E-4	0.0134729145803326	0.002500128478824606	0.0
18	2.77792053202734E-4	1.38896026601367E-4	0.013889602660136701	0.002500128478824606	0.0
19	2.77792053202734E-4	1.38896026601367E-4	0.014028498686738068	0.002500128478824606	0.0
20	2.77792053202734E-4	1.38896026601367E-4	0.014445186766542169	0.0027779205320273403	0.0
21	2.77792053202734E-4	1.38896026601367E-4	0.015139666899549004	0.0034724006650341754	0.0
22	2.77792053202734E-4	1.38896026601367E-4	0.015417458952751739	0.0037501927182369095	0.0
23	2.77792053202734E-4	1.38896026601367E-4	0.015417458952751739	0.004027984771439643	0.0
24	2.77792053202734E-4	1.38896026601367E-4	0.015417458952751739	0.00416688079804101	0.0
25	2.77792053202734E-4	1.38896026601367E-4	0.015417458952751739	0.00416688079804101	0.0
26	4.1668807980410106E-4	1.38896026601367E-4	0.015417458952751739	0.004583568877845111	0.0
27	4.1668807980410106E-4	1.38896026601367E-4	0.015417458952751739	0.0055558410640546806	0.0
28	4.1668807980410106E-4	1.38896026601367E-4	0.015417458952751739	0.0130562265005285	0.0
29	4.1668807980410106E-4	1.38896026601367E-4	0.015417458952751739	0.02930706161288844	0.0
30	5.55584106405468E-4	1.38896026601367E-4	0.015417458952751739	0.05250269805531673	0.0
31	5.55584106405468E-4	1.38896026601367E-4	0.015417458952751739	0.11181130141410045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	810	0.0	27.407408	1
GCCGCTC	165	0.0	23.545454	27
TGCTCGC	200	0.0	23.125002	10
CCGAATT	40	0.00193049	23.125	14
GTATCAA	2215	0.0	22.801355	1
GCCGGCA	185	0.0	22.0	15
GTCGCCC	120	1.0913936E-11	21.583332	37
TTCGCCG	180	0.0	21.583332	24
CCGGCAG	195	0.0	20.871796	16
CTGCTCG	355	0.0	20.84507	9
GTCTAGA	65	6.897552E-5	19.923077	1
CTAACTA	65	6.897552E-5	19.923077	9
CGCTCTC	190	0.0	19.473682	29
AGCTTCG	200	0.0	19.425001	21
CGGCAGC	220	0.0	19.340908	17
GCTTCGC	205	0.0	18.951221	22
CCGCTCT	200	0.0	18.5	28
GCCGATC	50	0.00703239	18.5	13
AACCTCG	50	0.00703239	18.5	5
CATGCTA	200	0.0	17.575	4
>>END_MODULE