FastQCFastQC Report
Fri 10 Feb 2017
ERR1633168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597645
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23020.38517849224874295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.25784537643584404No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13940.23324883501075053No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12120.20279597419873002No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12030.2012900634992345No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11940.199784152799739No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11680.19543374411230746No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9820.1643115896560667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9110.15243162747115763No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8010.13402605225510128No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7930.1326874649666608No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7820.13084690744505517No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7520.12582720511340345No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7430.12432129441390792No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7300.12214609007019217No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7150.11963623890436631No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6710.11227400881794376No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6610.11060077470739318No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6520.10909486400789765No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6410.10725430648629204No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6390.10691965966418192No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6360.10641768943101673No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6040.1010633402772549No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6020.10072869345514478No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6000.10039404663303465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA200.001841228837.034
TCATTAG250.00549425829.61
GGTATCA4950.029.1515121
ACTGCGG400.001930107823.12500233
GGTTGCG400.001930107823.1250028
CGTCCTA502.699999E-422.233
CGAGTTC603.7224338E-521.58333414
CCGAGTT603.7224338E-521.58333413
GTATCAA12050.021.340251
TAACGGC3150.021.14285936
TCGGAGC806.948612E-720.81253
GAGTGTC450.003823691520.5555557
TTAACGG3250.020.49230635
AACGGCC3350.020.43283737
TCTATAC1001.2867531E-820.353
GCACCGC3600.020.04166610
GGCACCG3750.019.7333359
GTATTAT951.6727427E-719.4736841
CACATGT3650.019.26027328
TGGGGCG1551.8189894E-1219.09677537
Produced by FastQC (version 0.11.5)