##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597645
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649214834893623	33.0	33.0	33.0	27.0	33.0
2	31.81057483957868	33.0	33.0	33.0	27.0	33.0
3	31.545343807778867	33.0	33.0	33.0	27.0	33.0
4	31.843197885032083	33.0	33.0	33.0	27.0	33.0
5	31.9436839595412	33.0	33.0	33.0	33.0	33.0
6	35.04583657522442	37.0	37.0	37.0	33.0	37.0
7	35.203935446628016	37.0	37.0	37.0	33.0	37.0
8	35.32848095441274	37.0	37.0	37.0	33.0	37.0
9	35.4588693957115	37.0	37.0	37.0	33.0	37.0
10	35.46964669661756	37.0	37.0	37.0	33.0	37.0
11	35.48620000167323	37.0	37.0	37.0	33.0	37.0
12	35.47269198269876	37.0	37.0	37.0	33.0	37.0
13	35.473966987090996	37.0	37.0	37.0	33.0	37.0
14	35.46180424834141	37.0	37.0	37.0	33.0	37.0
15	35.459314475984904	37.0	37.0	37.0	33.0	37.0
16	35.43431635837328	37.0	37.0	37.0	33.0	37.0
17	35.44870951819224	37.0	37.0	37.0	33.0	37.0
18	35.411260865564	37.0	37.0	37.0	33.0	37.0
19	35.40847660400405	37.0	37.0	37.0	33.0	37.0
20	35.41584720026103	37.0	37.0	37.0	33.0	37.0
21	35.429925792067195	37.0	37.0	37.0	33.0	37.0
22	35.32954847777527	37.0	37.0	37.0	33.0	37.0
23	35.36678462967146	37.0	37.0	37.0	33.0	37.0
24	35.35258891147755	37.0	37.0	37.0	33.0	37.0
25	35.3819675559906	37.0	37.0	37.0	33.0	37.0
26	35.2643090798049	37.0	37.0	37.0	33.0	37.0
27	35.27282918789582	37.0	37.0	37.0	33.0	37.0
28	35.29769512001272	37.0	37.0	37.0	33.0	37.0
29	35.31032971078148	37.0	37.0	37.0	33.0	37.0
30	35.30180960269056	37.0	37.0	37.0	33.0	37.0
31	35.291333483924404	37.0	37.0	37.0	33.0	37.0
32	35.27229709944867	37.0	37.0	37.0	33.0	37.0
33	35.2811200629136	37.0	37.0	37.0	33.0	37.0
34	35.272912849601354	37.0	37.0	37.0	33.0	37.0
35	35.20329292472957	37.0	37.0	37.0	33.0	37.0
36	35.23403023534038	37.0	37.0	37.0	33.0	37.0
37	35.242677509223704	37.0	37.0	37.0	33.0	37.0
38	35.23512118398045	37.0	37.0	37.0	33.0	37.0
39	35.12834040274745	37.0	37.0	37.0	33.0	37.0
40	34.92965389152423	37.0	37.0	37.0	27.0	37.0
41	35.104687565360706	37.0	37.0	37.0	27.0	37.0
42	35.140302353403776	37.0	37.0	37.0	33.0	37.0
43	34.79931397401467	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	20.0
16	41.0
17	35.0
18	16.0
19	24.0
20	54.0
21	145.0
22	378.0
23	815.0
24	1746.0
25	2897.0
26	4569.0
27	6837.0
28	9440.0
29	12632.0
30	16377.0
31	20584.0
32	26010.0
33	34473.0
34	49184.0
35	97925.0
36	313439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36602832785349	20.53593688560935	13.311581289896177	24.786453496640984
2	16.7363568673711	20.888989282935523	38.74691497460867	23.62773887508471
3	17.651281279020154	27.0582034485355	29.688360146909954	25.602155125534388
4	12.469944532289237	17.62635009077295	39.64845351337332	30.255251863564492
5	13.616277221427437	37.291033975018614	35.75617632541057	13.33651247814338
6	30.057140944875304	39.842214023375085	16.949694216466295	13.150950815283322
7	27.224020948891063	31.858210141471943	23.61954002794301	17.298228881693984
8	24.599553246492484	36.19673886671853	20.1042424850873	19.09946540170168
9	26.010926218741897	15.340377648938752	20.197943595278133	38.450752537041225
10	15.52025031582294	27.84947585941487	33.702281454709734	22.927992370052454
11	34.15790310301266	23.69834935454994	23.652168093098748	18.491579449338655
12	23.55210869328782	26.07283588083227	29.19559270135281	21.179462724527102
13	27.091500807335457	22.308895749148743	26.007245103698683	24.592358339817117
14	21.903638447573393	20.68970710036895	27.547624425871547	29.85903002618611
15	24.27712103338939	28.44029482385028	24.667486551380836	22.6150975913795
16	23.323712237197665	27.965096336453914	24.88433769210819	23.826853734240228
17	22.443925741870174	27.605518326096597	26.03284558559011	23.917710346443123
18	22.770541040249647	27.52219126739118	27.661404345388984	22.04586334697019
19	25.048983928586367	25.828711024102937	27.103213446109315	22.01909160120138
20	23.993173204828956	26.357285679625864	27.649022412970908	22.000518702574272
21	23.186339716721466	26.88970877360306	27.45475993273599	22.469191576939487
22	22.96948857599411	26.928527804967832	27.12145169791431	22.980531921123745
23	22.89017727915401	26.90125408896586	27.750587723481328	22.4579809083988
24	23.40653732566992	27.444720528072686	25.851299684595368	23.297442461662023
25	23.115227267023066	26.83064360950062	27.631955425043298	22.422173698433014
26	23.33843669737051	26.98993549682504	27.74255619975069	21.92907160605376
27	23.233022948405825	26.989098879769763	26.1352475131558	23.642630658668608
28	22.351395895556728	26.479766416518167	28.294388809410272	22.874448878514837
29	24.58466146290858	26.278309029607875	26.841184984397092	22.295844523086448
30	23.555120514686813	26.116507291117635	27.576738699395126	22.751633494800423
31	22.888671368454517	26.69795614453396	27.721640773368804	22.691731713642717
32	22.074810297082717	26.29822051552343	27.862694408888224	23.764274778505634
33	22.284466531134704	26.633201984455656	28.098955065297957	22.983376419111682
34	23.349145395678036	26.349254155895224	27.901513440252995	22.40008700817375
35	23.34395836993533	27.117770582871103	27.038626609442062	22.49964443775151
36	24.274443858812507	26.735436588610295	26.451656083460918	22.538463469116284
37	22.9758468656142	26.15131056061709	27.216826042215697	23.65601653155301
38	23.539559437458692	26.65512135130387	27.089492926402798	22.71582628483464
39	22.442085184348567	26.859423236202097	27.53172870182132	23.166762877628024
40	23.07841611659095	27.257987601335245	27.645675944749808	22.017920337323996
41	22.529260681508255	26.088898928293553	27.748579842548672	23.633260547649524
42	22.21117887709259	27.40456290941947	27.33830283864167	23.045955374846272
43	23.61987467476512	25.522676505283236	27.31320432698341	23.544244492968232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	42.0
2	47.0
3	143.0
4	239.0
5	239.0
6	285.0
7	331.0
8	313.5
9	296.0
10	360.5
11	425.0
12	425.0
13	740.5
14	1056.0
15	1967.5
16	2879.0
17	3369.0
18	3859.0
19	3859.0
20	3658.0
21	3457.0
22	3763.5
23	4070.0
24	5407.5
25	6745.0
26	6745.0
27	8970.0
28	11195.0
29	13402.0
30	15609.0
31	18633.5
32	21658.0
33	21658.0
34	27120.5
35	32583.0
36	35881.0
37	39179.0
38	42588.5
39	45998.0
40	45998.0
41	47650.5
42	49303.0
43	50311.5
44	51320.0
45	49260.5
46	47201.0
47	47201.0
48	44207.5
49	41214.0
50	41618.5
51	42023.0
52	39571.0
53	37119.0
54	37119.0
55	37341.5
56	37564.0
57	31905.0
58	26246.0
59	23938.0
60	21630.0
61	21630.0
62	20103.5
63	18577.0
64	14848.0
65	11119.0
66	9145.5
67	7172.0
68	7172.0
69	6041.5
70	4911.0
71	4341.5
72	3772.0
73	3831.0
74	3890.0
75	3890.0
76	3326.0
77	2762.0
78	2041.0
79	1320.0
80	820.0
81	320.0
82	320.0
83	269.0
84	218.0
85	190.0
86	162.0
87	127.5
88	93.0
89	93.0
90	64.0
91	35.0
92	22.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	597645.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.385249567042344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.34327529025192	44.367707492254354
2	7.439610133276559	7.645724467598264
3	1.9523059038957105	3.009591783086738
4	0.9360404692190372	1.9239469246268661
5	0.5726269964378939	1.4712290560393526
6	0.4008643500183802	1.2359108800934813
7	0.2640145084994414	0.9496515985994676
8	0.2241944174866893	0.921622887527297
9	0.18438005857226725	0.8526973792429655
>10	1.354276363857314	13.898417656601273
>50	0.18502056854783142	6.660562414297249
>100	0.1321575483289918	12.308085021889607
>500	0.008920634512206944	3.0547384553686316
>1k	0.0023127570957573555	1.7001139827744944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2302	0.38517849224874295	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1541	0.25784537643584404	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1394	0.23324883501075053	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1212	0.20279597419873002	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1203	0.2012900634992345	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1194	0.199784152799739	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1168	0.19543374411230746	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	982	0.1643115896560667	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.15243162747115763	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	801	0.13402605225510128	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	793	0.1326874649666608	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	782	0.13084690744505517	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	752	0.12582720511340345	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	743	0.12432129441390792	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	730	0.12214609007019217	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	715	0.11963623890436631	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	671	0.11227400881794376	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	661	0.11060077470739318	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	652	0.10909486400789765	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	641	0.10725430648629204	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	639	0.10691965966418192	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	636	0.10641768943101673	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	604	0.1010633402772549	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	602	0.10072869345514478	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	600	0.10039404663303465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.6732341105505776E-4	0.0	0.0
2	0.0	0.0	1.6732341105505776E-4	0.0	0.0
3	0.0	0.0	1.6732341105505776E-4	0.0	0.0
4	0.0	0.0	1.6732341105505776E-4	1.6732341105505776E-4	0.0
5	0.0	0.0	3.346468221101155E-4	1.6732341105505776E-4	0.0
6	0.0	0.0	5.019702331651733E-4	1.6732341105505776E-4	0.0
7	0.0	0.0	6.69293644220231E-4	5.019702331651733E-4	0.0
8	0.0	0.0	0.0011712638773854044	5.019702331651733E-4	0.0
9	0.0	0.0	0.002007880932660693	6.69293644220231E-4	0.0
10	0.0	0.0	0.002175204343715751	6.69293644220231E-4	0.0
11	0.0	0.0	0.003681115043211271	6.69293644220231E-4	0.0
12	0.0	0.0	0.00451773209848656	8.366170552752888E-4	0.0
13	0.0	0.0	0.004685055509541618	8.366170552752888E-4	0.0
14	0.0	0.0	0.005019702331651733	8.366170552752888E-4	0.0
15	0.0	0.0	0.005521672564816906	8.366170552752888E-4	0.0
16	0.0	0.0	0.0056889959758719645	8.366170552752888E-4	1.6732341105505776E-4
17	0.0	0.0	0.00602364279798208	8.366170552752888E-4	1.6732341105505776E-4
18	0.0	0.0	0.00602364279798208	8.366170552752888E-4	1.6732341105505776E-4
19	0.0	0.0	0.00602364279798208	8.366170552752888E-4	1.6732341105505776E-4
20	0.0	0.0	0.006190966209037137	8.366170552752888E-4	1.6732341105505776E-4
21	0.0	0.0	0.006358289620092195	8.366170552752888E-4	1.6732341105505776E-4
22	0.0	0.0	0.006525613031147253	0.00150591069949552	1.6732341105505776E-4
23	0.0	0.0	0.006525613031147253	0.0016732341105505776	1.6732341105505776E-4
24	0.0	0.0	0.00669293644220231	0.002175204343715751	1.6732341105505776E-4
25	0.0	0.0	0.00669293644220231	0.0025098511658258665	1.6732341105505776E-4
26	0.0	0.0	0.00669293644220231	0.0028444979879359822	1.6732341105505776E-4
27	0.0	0.0	0.00669293644220231	0.003346468221101155	1.6732341105505776E-4
28	0.0	0.0	0.00669293644220231	0.01121066854068887	1.6732341105505776E-4
29	3.346468221101155E-4	0.0	0.00669293644220231	0.023592600958763147	1.6732341105505776E-4
30	3.346468221101155E-4	0.0	0.00669293644220231	0.04467535075170043	1.6732341105505776E-4
31	3.346468221101155E-4	0.0	0.00669293644220231	0.09353378677977729	1.6732341105505776E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGTA	20	0.0018412288	37.0	34
TCATTAG	25	0.005494258	29.6	1
GGTATCA	495	0.0	29.151512	1
ACTGCGG	40	0.0019301078	23.125002	33
GGTTGCG	40	0.0019301078	23.125002	8
CGTCCTA	50	2.699999E-4	22.2	33
CGAGTTC	60	3.7224338E-5	21.583334	14
CCGAGTT	60	3.7224338E-5	21.583334	13
GTATCAA	1205	0.0	21.34025	1
TAACGGC	315	0.0	21.142859	36
TCGGAGC	80	6.948612E-7	20.8125	3
GAGTGTC	45	0.0038236915	20.555555	7
TTAACGG	325	0.0	20.492306	35
AACGGCC	335	0.0	20.432837	37
TCTATAC	100	1.2867531E-8	20.35	3
GCACCGC	360	0.0	20.041666	10
GGCACCG	375	0.0	19.733335	9
GTATTAT	95	1.6727427E-7	19.473684	1
CACATGT	365	0.0	19.260273	28
TGGGGCG	155	1.8189894E-12	19.096775	37
>>END_MODULE