FastQCFastQC Report
Fri 10 Feb 2017
ERR1633167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845231
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44550.5270748469944903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.3550508677509462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17360.20538763959201686No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12890.1525026886141185No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12800.15143789094342255No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12210.14445755065774918No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11630.13759552122437535No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10590.12529119258522226No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10560.12493626002832363No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8680.10269381979600843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.027.3668651
CGAGTCG1051.8189894E-1224.66666621
TATACGT502.7012813E-422.24
GTATCAA21200.021.3797171
AGTCGCC1059.822543E-1021.14285723
TATCGCC450.00382498220.55555733
GTATTAT1500.019.7333341
TTAACGG3100.019.09677535
GTCAACC2950.018.81355915
GCCGGCA1700.018.515
CCGTCCG1103.848072E-818.528
CCGTCCA500.00703337718.59
TAGCTCG1205.167749E-918.536
TAGTCGG500.00703337718.530
CAACCCA3250.018.21538417
GAGTCGC1258.569259E-917.7622
GCTCTAG1054.7935464E-717.6190471
CGTCCGC1054.7935464E-717.61904729
CCGAGTT953.604102E-617.52631613
ACGCCGA650.00157946617.07692336
Produced by FastQC (version 0.11.5)