##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633167.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845231
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816595699873762	33.0	33.0	33.0	27.0	33.0
2	31.941108407050855	33.0	33.0	33.0	33.0	33.0
3	31.660025484157586	33.0	33.0	33.0	27.0	33.0
4	31.954908184863072	33.0	33.0	33.0	33.0	33.0
5	32.04987748911245	33.0	33.0	33.0	33.0	33.0
6	35.20753853088682	37.0	37.0	37.0	33.0	37.0
7	35.350860297362495	37.0	37.0	37.0	33.0	37.0
8	35.483362536395376	37.0	37.0	37.0	33.0	37.0
9	35.598418657148166	37.0	37.0	37.0	33.0	37.0
10	35.59137797832782	37.0	37.0	37.0	33.0	37.0
11	35.61934903002848	37.0	37.0	37.0	33.0	37.0
12	35.59298937213614	37.0	37.0	37.0	33.0	37.0
13	35.60550666030943	37.0	37.0	37.0	33.0	37.0
14	35.57571362148336	37.0	37.0	37.0	33.0	37.0
15	35.59817020435833	37.0	37.0	37.0	33.0	37.0
16	35.58233547988657	37.0	37.0	37.0	33.0	37.0
17	35.57454352715411	37.0	37.0	37.0	33.0	37.0
18	35.56701304140525	37.0	37.0	37.0	33.0	37.0
19	35.5494817393115	37.0	37.0	37.0	33.0	37.0
20	35.55033476055658	37.0	37.0	37.0	33.0	37.0
21	35.56263080743607	37.0	37.0	37.0	33.0	37.0
22	35.46733259901731	37.0	37.0	37.0	33.0	37.0
23	35.51127324956136	37.0	37.0	37.0	33.0	37.0
24	35.487492768249155	37.0	37.0	37.0	33.0	37.0
25	35.522767148862265	37.0	37.0	37.0	33.0	37.0
26	35.41918836389105	37.0	37.0	37.0	33.0	37.0
27	35.42559962897717	37.0	37.0	37.0	33.0	37.0
28	35.449402589351315	37.0	37.0	37.0	33.0	37.0
29	35.44954456237407	37.0	37.0	37.0	33.0	37.0
30	35.44157277714613	37.0	37.0	37.0	33.0	37.0
31	35.44245300988724	37.0	37.0	37.0	33.0	37.0
32	35.42937847759961	37.0	37.0	37.0	33.0	37.0
33	35.425160695715135	37.0	37.0	37.0	33.0	37.0
34	35.413516541631815	37.0	37.0	37.0	33.0	37.0
35	35.34223661933838	37.0	37.0	37.0	33.0	37.0
36	35.373489614081834	37.0	37.0	37.0	33.0	37.0
37	35.37065961849483	37.0	37.0	37.0	33.0	37.0
38	35.359530116619005	37.0	37.0	37.0	33.0	37.0
39	35.264579742106	37.0	37.0	37.0	33.0	37.0
40	35.05147113629292	37.0	37.0	37.0	27.0	37.0
41	35.223579116241595	37.0	37.0	37.0	33.0	37.0
42	35.25422044387866	37.0	37.0	37.0	33.0	37.0
43	34.92835449717296	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	25.0
16	42.0
17	52.0
18	33.0
19	41.0
20	59.0
21	157.0
22	412.0
23	991.0
24	1978.0
25	3468.0
26	5757.0
27	8504.0
28	12198.0
29	15992.0
30	21308.0
31	26990.0
32	34572.0
33	44887.0
34	65570.0
35	132508.0
36	469685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.637966425746335	20.177205994574265	13.645618771673067	24.539208808006332
2	16.43349569525964	21.189000403440005	39.04897004487531	23.32853385642505
3	17.748165886012227	27.459357264463797	29.862723918076835	24.929752931447144
4	12.416605637985354	17.786380291304983	39.93547326115582	29.861540809553837
5	13.788301659546326	37.03520102788468	35.30135548743479	13.875141825134193
6	29.277795064307867	40.145948267396726	17.16962581826743	13.406630850027982
7	26.658037861838952	32.14860789535642	23.4464897761677	17.74686446663693
8	24.64971114405411	35.654513381548945	20.166912950424205	19.528862523972737
9	26.302158818121914	15.19217823293277	20.10479975296694	38.40086319597838
10	15.604609864049	27.65315044053046	33.447661053605465	23.29457864181508
11	34.00478685708404	23.87039755995698	23.673646612582832	18.451168970376145
12	23.46506457997873	25.991356209130995	29.41408916615694	21.129490044733334
13	28.099892218813554	22.097036194839045	25.918476724114477	23.884594862232927
14	22.32300992273118	20.800112631931388	27.6693590272955	29.207518418041932
15	24.32932535602693	28.165436430987505	24.824456272900544	22.68078194008502
16	22.871144101434993	27.992229343220963	25.1502843601335	23.98634219521054
17	22.522363708855924	27.221434140489404	25.97218985105847	24.284012299596206
18	23.350894607509662	26.65898434865735	27.622862862341773	22.367258181491216
19	25.403942827463734	25.639499734392135	27.250538610155097	21.706018827989034
20	24.541693335904622	25.810103983408084	27.569977911363875	22.078224769323416
21	23.432055852187155	26.698263551620798	27.397125756154235	22.472554840037812
22	23.087298028586268	26.371015734160245	27.449182531166038	23.09250370608745
23	23.046599095395223	26.362970596203876	28.00181252225723	22.58861778614367
24	23.20785678707951	26.98374763821961	26.42851480837783	23.37988076632305
25	23.204070839805922	26.59888243568918	27.856053552224186	22.34099317228071
26	23.46246174122814	26.69211138730122	27.617302252283693	22.228124619186946
27	23.452641940487275	26.5929668930742	26.720032748443916	23.234358417994606
28	22.64883800996414	26.28346570345858	28.07469200727375	22.993004279303527
29	24.262716346182287	26.16397174263604	26.993685749812773	22.579626161368903
30	23.712689193841683	26.061751166249223	27.54264810448268	22.68291153542641
31	23.225011860662942	26.19603398360921	27.622862862341773	22.95609129338607
32	22.235933135438714	26.211887637817355	27.804233398916985	23.74794582782695
33	22.46770409509353	26.43230075565141	28.04677064613106	23.053224503124
34	23.27553059459485	26.126940445866275	27.796543193517515	22.80098576602136
35	23.311023850284716	26.68063523462817	27.222735559864695	22.78560535522242
36	24.058275193408665	26.55652715056594	26.58527668767473	22.799920968350666
37	23.239919028052686	25.99821823856437	27.232437049753262	23.529425683629682
38	23.603961520578398	26.36805796285276	26.99912804901855	23.02885246755029
39	22.750467032089453	26.432537377356013	27.614344480976204	23.20265110957833
40	23.15177744308952	26.786286825731665	27.569859600511577	22.492076130667236
41	22.959995551511952	25.73225544259498	27.640491179334408	23.667257826558657
42	22.430081244062276	27.18830710184553	27.231963806344066	23.14964784774813
43	23.376804684163265	25.713325706227053	27.443976853664857	23.465892755944825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	39.0
2	51.0
3	247.5
4	444.0
5	444.0
6	538.5
7	633.0
8	562.0
9	491.0
10	743.5
11	996.0
12	996.0
13	1532.0
14	2068.0
15	3677.5
16	5287.0
17	6121.5
18	6956.0
19	6956.0
20	6228.5
21	5501.0
22	5698.5
23	5896.0
24	7589.0
25	9282.0
26	9282.0
27	12157.0
28	15032.0
29	18204.5
30	21377.0
31	25267.0
32	29157.0
33	29157.0
34	36193.5
35	43230.0
36	46932.5
37	50635.0
38	55202.5
39	59770.0
40	59770.0
41	63064.0
42	66358.0
43	68583.0
44	70808.0
45	69468.0
46	68128.0
47	68128.0
48	65571.0
49	63014.0
50	62997.0
51	62980.0
52	59615.5
53	56251.0
54	56251.0
55	55292.0
56	54333.0
57	46592.5
58	38852.0
59	35458.5
60	32065.0
61	32065.0
62	28704.5
63	25344.0
64	20575.5
65	15807.0
66	13103.0
67	10399.0
68	10399.0
69	8805.0
70	7211.0
71	6316.5
72	5422.0
73	5225.0
74	5028.0
75	5028.0
76	4297.5
77	3567.0
78	2612.0
79	1657.0
80	1087.0
81	517.0
82	517.0
83	401.0
84	285.0
85	245.5
86	206.0
87	155.5
88	105.0
89	105.0
90	75.0
91	45.0
92	28.5
93	12.0
94	7.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	845231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.736245638063394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.8839533668402	42.21809155383083
2	8.524687928333295	8.47971945582837
3	2.174042653442569	3.2438615831774023
4	1.0116883267917314	2.0127031652189964
5	0.5875712332699021	1.461179359388585
6	0.37833872617304143	1.1290288691600638
7	0.2884237053409071	1.0041578579673043
8	0.2181205300805972	0.8678797010234542
9	0.17001579978257825	0.7610352822304306
>10	1.422127428701036	14.555294796603205
>50	0.1893808751943502	6.595647393568814
>100	0.14003990061072513	12.688917831658092
>500	0.009432739419226573	3.0264925174319823
>1k	0.0021767860198215164	1.9559906329124384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4455	0.5270748469944903	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3001	0.3550508677509462	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1736	0.20538763959201686	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1289	0.1525026886141185	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1280	0.15143789094342255	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1221	0.14445755065774918	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1163	0.13759552122437535	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1059	0.12529119258522226	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1056	0.12493626002832363	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	868	0.10269381979600843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.3662170459909777E-4	0.0	1.1831085229954889E-4
2	0.0	0.0	3.5493255689864663E-4	0.0	1.1831085229954889E-4
3	0.0	0.0	4.7324340919819555E-4	0.0	1.1831085229954889E-4
4	0.0	0.0	5.915542614977444E-4	1.1831085229954889E-4	1.1831085229954889E-4
5	0.0	0.0	8.281759660968422E-4	2.3662170459909777E-4	1.1831085229954889E-4
6	0.0	0.0	0.002247906193691429	2.3662170459909777E-4	1.1831085229954889E-4
7	0.0	0.0	0.004614123239682406	3.5493255689864663E-4	1.1831085229954889E-4
8	0.0	0.0	0.005205677501180151	3.5493255689864663E-4	2.3662170459909777E-4
9	1.1831085229954889E-4	0.0	0.006743718581074286	7.098651137972933E-4	2.3662170459909777E-4
10	2.3662170459909777E-4	0.0	0.007216961990272482	7.098651137972933E-4	2.3662170459909777E-4
11	2.3662170459909777E-4	0.0	0.011121220116157595	9.464868183963911E-4	2.3662170459909777E-4
12	2.3662170459909777E-4	0.0	0.012540950343752182	9.464868183963911E-4	2.3662170459909777E-4
13	2.3662170459909777E-4	0.0	0.013369126309849023	9.464868183963911E-4	2.3662170459909777E-4
14	2.3662170459909777E-4	0.0	0.013724058866747671	9.464868183963911E-4	2.3662170459909777E-4
15	2.3662170459909777E-4	0.0	0.014670545685144061	9.464868183963911E-4	2.3662170459909777E-4
16	2.3662170459909777E-4	0.0	0.01585365420813955	9.464868183963911E-4	2.3662170459909777E-4
17	2.3662170459909777E-4	0.0	0.01585365420813955	0.00106479767069594	2.3662170459909777E-4
18	2.3662170459909777E-4	0.0	0.01668183017423639	0.0013014193752950377	2.3662170459909777E-4
19	2.3662170459909777E-4	0.0	0.01691845187883549	0.0013014193752950377	2.3662170459909777E-4
20	2.3662170459909777E-4	0.0	0.01703676273113504	0.0013014193752950377	2.3662170459909777E-4
21	2.3662170459909777E-4	0.0	0.017273384435734136	0.0018929736367927822	2.3662170459909777E-4
22	2.3662170459909777E-4	0.0	0.017273384435734136	0.00212959534139188	2.3662170459909777E-4
23	2.3662170459909777E-4	0.0	0.017510006140333233	0.0026028387505900754	2.3662170459909777E-4
24	2.3662170459909777E-4	0.0	0.017510006140333233	0.003076082159788271	2.3662170459909777E-4
25	2.3662170459909777E-4	0.0	0.017746627844932333	0.0033127038643873687	2.3662170459909777E-4
26	2.3662170459909777E-4	0.0	0.017746627844932333	0.0036676364212860153	2.3662170459909777E-4
27	2.3662170459909777E-4	0.0	0.017746627844932333	0.004850744944281504	2.3662170459909777E-4
28	2.3662170459909777E-4	0.0	0.017746627844932333	0.01360574801444812	2.3662170459909777E-4
29	2.3662170459909777E-4	0.0	0.017746627844932333	0.029341091370288123	2.3662170459909777E-4
30	2.3662170459909777E-4	0.0	0.017746627844932333	0.05217508586410106	2.3662170459909777E-4
31	2.3662170459909777E-4	0.0	0.017746627844932333	0.11026571434317956	2.3662170459909777E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	27.366865	1
CGAGTCG	105	1.8189894E-12	24.666666	21
TATACGT	50	2.7012813E-4	22.2	4
GTATCAA	2120	0.0	21.379717	1
AGTCGCC	105	9.822543E-10	21.142857	23
TATCGCC	45	0.003824982	20.555557	33
GTATTAT	150	0.0	19.733334	1
TTAACGG	310	0.0	19.096775	35
GTCAACC	295	0.0	18.813559	15
GCCGGCA	170	0.0	18.5	15
CCGTCCG	110	3.848072E-8	18.5	28
CCGTCCA	50	0.007033377	18.5	9
TAGCTCG	120	5.167749E-9	18.5	36
TAGTCGG	50	0.007033377	18.5	30
CAACCCA	325	0.0	18.215384	17
GAGTCGC	125	8.569259E-9	17.76	22
GCTCTAG	105	4.7935464E-7	17.619047	1
CGTCCGC	105	4.7935464E-7	17.619047	29
CCGAGTT	95	3.604102E-6	17.526316	13
ACGCCGA	65	0.001579466	17.076923	36
>>END_MODULE