FastQCFastQC Report
Fri 10 Feb 2017
ERR1633166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367211
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26420.719477357704426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.5533603296197555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11800.3213411362949367No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5420.14759906429818279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.1290811005116949No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4310.11737121164670994No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3680.10021486284452262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA303.5947518E-430.83333233
GGTATCA4600.028.5543461
CGCTCTC358.8578573E-426.4285729
TACGTCC358.8578573E-426.428574
ACGGTTC358.8578573E-426.4285732
GCCGCTC509.069105E-625.90000227
CCGCTCT451.3208175E-424.66666828
GTATCAA12200.023.5040991
CCGGTTA400.001928698623.12523
TTCGCCG502.697252E-422.224
ATGCCCG450.00382092520.55555729
TATAACG450.00382092520.55555728
GCCGGCA555.1339366E-420.18181815
ACTGCTC1205.140464E-918.58
TGCCCGC500.007025989218.530
CCCTAGA500.007025989218.51
GCCGGTT500.007025989218.522
TAACGGT500.007025989218.530
TCTCGTC500.007025989218.512
TCGTCTT500.007025989218.514
Produced by FastQC (version 0.11.5)