##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367211
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.893478136548197	33.0	33.0	33.0	27.0	33.0
2	31.273235278899598	33.0	33.0	33.0	27.0	33.0
3	31.00755696316287	33.0	33.0	33.0	27.0	33.0
4	31.356124952683878	33.0	33.0	33.0	27.0	33.0
5	31.480402275530963	33.0	33.0	33.0	27.0	33.0
6	34.102940816043095	37.0	33.0	37.0	27.0	37.0
7	34.30276053821917	37.0	33.0	37.0	27.0	37.0
8	34.44407983420976	37.0	37.0	37.0	27.0	37.0
9	34.53767452500061	37.0	37.0	37.0	27.0	37.0
10	34.532233511523344	37.0	37.0	37.0	27.0	37.0
11	34.55002164967825	37.0	37.0	37.0	27.0	37.0
12	34.55503239282048	37.0	37.0	37.0	27.0	37.0
13	34.608955069428745	37.0	37.0	37.0	27.0	37.0
14	34.6191154404416	37.0	37.0	37.0	27.0	37.0
15	34.62370680616866	37.0	37.0	37.0	27.0	37.0
16	34.60468776806795	37.0	37.0	37.0	27.0	37.0
17	34.618050657523874	37.0	37.0	37.0	27.0	37.0
18	34.58896383822925	37.0	37.0	37.0	27.0	37.0
19	34.55014147179687	37.0	37.0	37.0	27.0	37.0
20	34.54822704112894	37.0	37.0	37.0	27.0	37.0
21	34.558964736895135	37.0	37.0	37.0	27.0	37.0
22	34.435763089885654	37.0	37.0	37.0	27.0	37.0
23	34.487836693345244	37.0	37.0	37.0	27.0	37.0
24	34.46584388811882	37.0	37.0	37.0	27.0	37.0
25	34.47709355111911	37.0	37.0	37.0	27.0	37.0
26	34.35173782920446	37.0	37.0	37.0	27.0	37.0
27	34.35707535994292	37.0	37.0	37.0	27.0	37.0
28	34.37757855837652	37.0	37.0	37.0	27.0	37.0
29	34.36410673972185	37.0	37.0	37.0	27.0	37.0
30	34.36901672335524	37.0	37.0	37.0	27.0	37.0
31	34.36526955891844	37.0	37.0	37.0	27.0	37.0
32	34.33129998829011	37.0	37.0	37.0	27.0	37.0
33	34.335986667066074	37.0	37.0	37.0	27.0	37.0
34	34.290056670415645	37.0	37.0	37.0	27.0	37.0
35	34.215369365296795	37.0	37.0	37.0	27.0	37.0
36	34.24707865505118	37.0	37.0	37.0	27.0	37.0
37	34.2318993712062	37.0	37.0	37.0	27.0	37.0
38	34.18474664429987	37.0	37.0	37.0	27.0	37.0
39	34.08691188444791	37.0	37.0	37.0	27.0	37.0
40	33.87690183572932	37.0	33.0	37.0	27.0	37.0
41	34.07166996631364	37.0	33.0	37.0	27.0	37.0
42	34.06931164916084	37.0	37.0	37.0	27.0	37.0
43	33.61351103316622	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	24.0
17	24.0
18	27.0
19	38.0
20	120.0
21	272.0
22	597.0
23	1336.0
24	2365.0
25	3663.0
26	5406.0
27	7250.0
28	9568.0
29	12485.0
30	15169.0
31	18862.0
32	22650.0
33	30031.0
34	41626.0
35	72972.0
36	122710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.594886863410956	21.217773977359066	14.086179335586351	23.101159823643627
2	16.181704796424945	23.232964154123923	38.600423189937125	21.984907859514013
3	18.529945998349724	28.32594884140181	30.432639545111666	22.711465615136802
4	12.970472017450458	19.18270422182342	39.14888170561339	28.69794205511273
5	14.051866637981977	37.43270217940095	34.83147291339312	13.683958269223961
6	27.934076048920105	41.07447761641127	17.519900002995552	13.471546331673071
7	25.29608317833616	33.50471527269063	23.330455786999845	17.86874576197336
8	24.23974227351577	35.84097426275357	20.798124239197627	19.121159224533034
9	26.221164398669977	15.421378989191501	20.504015402588703	37.85344120954982
10	14.808107600262518	28.683781259276003	33.67791269869367	22.830198441767813
11	33.20325371516649	24.717396810008417	23.988115824417026	18.091233650408075
12	22.768108798483706	26.735037893745012	30.575064472469503	19.921788835301772
13	28.46646750778163	23.067664095029834	26.555849361811056	21.91001903537748
14	21.986269474498314	21.98191230654855	29.001309873614893	27.030508345338237
15	23.701904354717044	29.003488457589775	25.59917867384147	21.69542851385171
16	21.64668269741375	29.25919975164143	25.974439763514713	23.119677787430113
17	21.733281410415266	27.968388746524475	26.878007467096577	23.420322375963686
18	22.797247359147736	26.787868555135873	28.664174003502076	21.750710082214315
19	24.769138179411836	26.31021401864323	28.82756780161814	20.093080000326786
20	24.44153361418912	25.948841401809858	28.897554811811194	20.71207017218983
21	22.602808739389616	27.345041406711672	28.646473008706163	21.405676845192545
22	21.914103880330384	27.3632870475013	28.741241411613487	21.98136766055483
23	22.107725531097923	26.979583944925395	29.390459436128	21.522231087848674
24	22.32803483555776	27.351304835639457	28.36516335294967	21.95549697585312
25	22.387401248873264	27.08524526770712	29.090631816585017	21.4367216668346
26	22.395843261775926	27.074896993826435	28.749955747513013	21.779303996884625
27	22.57339785572872	26.9863920198469	28.47191396771883	21.968296156705545
28	21.733553733412126	27.27124187456258	28.95882748610472	22.036376905920573
29	22.668166258636045	27.260348954688173	28.283194130894774	21.788290655781008
30	22.349820675306567	27.140799159066585	29.071841529801667	21.437538635825177
31	22.625411548129005	26.769895237343107	28.858340300263336	21.74635291426455
32	21.468583457467233	26.899520983848525	29.030448434278927	22.601447124405315
33	21.515695335924033	26.923757730569076	29.632554580336645	21.927992353170247
34	21.829139105310023	26.942275694355562	28.876313618056106	22.35227158227831
35	22.039100135889175	27.54601577839444	28.70311619205307	21.711767893663318
36	22.3816824659392	27.332514548856107	28.45993175585699	21.825871229347705
37	22.769470413468007	26.757913025481262	28.268761012061184	22.203855548989544
38	22.49850903159219	27.308277802135557	28.218653580638925	21.974559585633326
39	22.19214566012456	26.992383125777824	28.65055785365907	22.164913360438547
40	22.065243143587747	27.596940178807277	28.729531522748502	21.60828515485647
41	22.21093594690791	26.735582539738733	28.775281786221	22.278199727132357
42	21.829956074300608	27.29248306831767	28.667986525458115	22.209574331923605
43	22.165730329429127	26.920217531609893	28.502414143367165	22.411637995593814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	29.5
2	34.0
3	126.0
4	218.0
5	218.0
6	277.0
7	336.0
8	347.5
9	359.0
10	456.5
11	554.0
12	554.0
13	1004.0
14	1454.0
15	2693.0
16	3932.0
17	4330.0
18	4728.0
19	4728.0
20	4510.0
21	4292.0
22	4495.0
23	4698.0
24	5826.0
25	6954.0
26	6954.0
27	8388.5
28	9823.0
29	11516.0
30	13209.0
31	14772.5
32	16336.0
33	16336.0
34	18117.5
35	19899.0
36	20928.5
37	21958.0
38	23169.0
39	24380.0
40	24380.0
41	25359.5
42	26339.0
43	26184.0
44	26029.0
45	26187.5
46	26346.0
47	26346.0
48	26080.0
49	25814.0
50	26066.5
51	26319.0
52	26218.5
53	26118.0
54	26118.0
55	23850.5
56	21583.0
57	19181.5
58	16780.0
59	14711.0
60	12642.0
61	12642.0
62	11014.0
63	9386.0
64	7652.0
65	5918.0
66	4922.5
67	3927.0
68	3927.0
69	3265.0
70	2603.0
71	2228.0
72	1853.0
73	1471.5
74	1090.0
75	1090.0
76	783.5
77	477.0
78	388.5
79	300.0
80	226.0
81	152.0
82	152.0
83	140.0
84	128.0
85	117.0
86	106.0
87	77.0
88	48.0
89	48.0
90	48.0
91	48.0
92	31.0
93	14.0
94	8.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	367211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.11708838854166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99884861244122	57.70464131350873
2	5.384750200295172	6.905090090850859
3	1.3547170670434578	2.6058154178727393
4	0.6903674662768972	1.7705740742339746
5	0.458373582197801	1.4694789742374437
6	0.3080176743326931	1.1849517870253397
7	0.22158660065482766	0.9945241361931448
8	0.16812081309559812	0.8623533626563165
9	0.14646954429519593	0.8452080646024046
>10	1.1187456889380727	14.466061762432695
>50	0.09756625887507135	4.312274767409406
>100	0.050717262322673276	5.116417007391479
>500	4.29807307819265E-4	0.1493643228485501
>1k	0.001289421923457795	1.613244918736923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2642	0.719477357704426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2032	0.5533603296197555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.3213411362949367	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	542	0.14759906429818279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.1290811005116949	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	431	0.11737121164670994	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	368	0.10021486284452262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.7232299686011586E-4	0.0	0.0
2	0.0	0.0	2.7232299686011586E-4	0.0	0.0
3	0.0	0.0	5.446459937202317E-4	0.0	0.0
4	0.0	0.0	5.446459937202317E-4	2.7232299686011586E-4	0.0
5	0.0	0.0	8.169689905803475E-4	2.7232299686011586E-4	0.0
6	0.0	0.0	0.0010892919874404634	2.7232299686011586E-4	0.0
7	0.0	0.0	0.002178583974880927	2.7232299686011586E-4	0.0
8	0.0	0.0	0.0024509069717410428	2.7232299686011586E-4	0.0
9	0.0	0.0	0.003540198959181506	0.0010892919874404634	0.0
10	2.7232299686011586E-4	0.0	0.003812521956041622	0.0013616149843005793	0.0
11	2.7232299686011586E-4	0.0	0.005174136940342201	0.0013616149843005793	0.0
12	2.7232299686011586E-4	0.0	0.005446459937202317	0.0013616149843005793	0.0
13	2.7232299686011586E-4	0.0	0.005991105930922548	0.001633937981160695	0.0
14	2.7232299686011586E-4	0.0	0.00653575192464278	0.001633937981160695	0.0
15	2.7232299686011586E-4	0.0	0.007352720915223128	0.001633937981160695	0.0
16	2.7232299686011586E-4	0.0	0.008442012902663592	0.001633937981160695	0.0
17	2.7232299686011586E-4	0.0	0.008442012902663592	0.001633937981160695	0.0
18	2.7232299686011586E-4	0.0	0.008442012902663592	0.001633937981160695	0.0
19	2.7232299686011586E-4	0.0	0.008714335899523707	0.001633937981160695	0.0
20	2.7232299686011586E-4	0.0	0.009531304890104054	0.001633937981160695	0.0
21	2.7232299686011586E-4	0.0	0.010620596877544517	0.001633937981160695	0.0
22	2.7232299686011586E-4	0.0	0.010620596877544517	0.002178583974880927	0.0
23	2.7232299686011586E-4	0.0	0.010892919874404635	0.0027232299686011587	0.0
24	2.7232299686011586E-4	0.0	0.010892919874404635	0.003812521956041622	0.0
25	2.7232299686011586E-4	0.0	0.010892919874404635	0.003812521956041622	0.0
26	5.446459937202317E-4	0.0	0.010892919874404635	0.004084844952901737	0.0
27	5.446459937202317E-4	0.0	0.010892919874404635	0.005174136940342201	0.0
28	5.446459937202317E-4	0.0	0.010892919874404635	0.010892919874404635	0.0
29	5.446459937202317E-4	0.0	0.010892919874404635	0.027232299686011586	0.0
30	5.446459937202317E-4	0.0	0.010892919874404635	0.048745816437960734	0.0
31	5.446459937202317E-4	0.0	0.010892919874404635	0.09531304890104055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCA	30	3.5947518E-4	30.833332	33
GGTATCA	460	0.0	28.554346	1
CGCTCTC	35	8.8578573E-4	26.42857	29
TACGTCC	35	8.8578573E-4	26.42857	4
ACGGTTC	35	8.8578573E-4	26.42857	32
GCCGCTC	50	9.069105E-6	25.900002	27
CCGCTCT	45	1.3208175E-4	24.666668	28
GTATCAA	1220	0.0	23.504099	1
CCGGTTA	40	0.0019286986	23.125	23
TTCGCCG	50	2.697252E-4	22.2	24
ATGCCCG	45	0.003820925	20.555557	29
TATAACG	45	0.003820925	20.555557	28
GCCGGCA	55	5.1339366E-4	20.181818	15
ACTGCTC	120	5.140464E-9	18.5	8
TGCCCGC	50	0.0070259892	18.5	30
CCCTAGA	50	0.0070259892	18.5	1
GCCGGTT	50	0.0070259892	18.5	22
TAACGGT	50	0.0070259892	18.5	30
TCTCGTC	50	0.0070259892	18.5	12
TCGTCTT	50	0.0070259892	18.5	14
>>END_MODULE