FastQCFastQC Report
Fri 10 Feb 2017
ERR1633165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367120
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24800.6755284375680977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.5137284811505775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11940.32523425582915666No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5870.1598932229243844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.1321093920244062No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3990.10868380910873827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC200.001840304737.06
GTGTCGT200.001840304737.06
GGTATCA6400.028.3281251
TGTCCGG451.3208165E-424.66666814
CGAGTCG450.00382092320.55555721
GTATCAA14000.019.9535711
GTAAGAC656.885787E-519.9230773
TAACTAG656.885787E-519.92307726
TCACCCG500.00702598718.55
CTATACC1103.830428E-818.54
GTACATG1301.384069E-817.0769231
GTCCGGC650.001577166117.07692315
ATAGGTT2300.016.89130417
TAGGTTA2300.016.89130418
TAACTGA2350.016.5319166
ACCAACG904.4327076E-516.44444530
CGCTTCC904.4327076E-516.44444526
CATAGGT2400.016.187516
GGCGAAC2400.016.187529
CATGCTA1403.450441E-815.8571424
Produced by FastQC (version 0.11.5)