FastQCFastQC Report
Fri 10 Feb 2017
ERR1633164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003902
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43330.4316158350117841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29370.2925584369789083No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19020.18946072425396104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17320.17252680042474264No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC17240.17172990989160297No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC16600.16535478562648545No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14440.14383874123171386No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13580.13527216800046218No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13270.1321842171845459No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13100.13049082480162405No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT12060.12013124787080812No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT11620.11574834993853982No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA11380.11335767833912075No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC11160.11116622937298659No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10990.10947283699006477No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10570.10528916169108139No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10540.10499032774115402No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA10380.10339654667487463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA250.005496016229.628
GGTATCA9950.026.7738691
TTCGCCG1350.023.29629524
CGATCGC400.001931017423.12500210
CGCTCTC1450.021.68965529
GTATCAA25150.021.6262421
CCGCTCT1500.020.96666728
GGACCGT1151.3460522E-1020.9130446
TGCGTTT806.9583075E-720.812524
ATACGCC450.003825476220.5555556
CGATCCT450.003825476220.55555533
AATACGC450.003825476220.5555555
CCGGCAG1750.020.08571416
AGCTTCG1950.019.92307521
GCGTTTA759.263853E-619.73333425
GCCGGCA1900.019.47368415
GCTTCGC1950.018.97435822
TCGGGTA500.007034276618.525
AACGGCC2100.018.537
ACGTGCA500.007034276618.59
Produced by FastQC (version 0.11.5)