##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633164.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003902
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850173622524906	33.0	33.0	33.0	33.0	33.0
2	31.966552512097795	33.0	33.0	33.0	33.0	33.0
3	31.68157648854171	33.0	33.0	33.0	27.0	33.0
4	31.96825885395188	33.0	33.0	33.0	33.0	33.0
5	32.07195423457668	33.0	33.0	33.0	33.0	33.0
6	35.23769850045124	37.0	37.0	37.0	33.0	37.0
7	35.36963667768368	37.0	37.0	37.0	33.0	37.0
8	35.48824785686252	37.0	37.0	37.0	33.0	37.0
9	35.60570553699465	37.0	37.0	37.0	33.0	37.0
10	35.6149454827264	37.0	37.0	37.0	33.0	37.0
11	35.63281874127156	37.0	37.0	37.0	33.0	37.0
12	35.6144085777297	37.0	37.0	37.0	33.0	37.0
13	35.62109847375541	37.0	37.0	37.0	33.0	37.0
14	35.59678335136298	37.0	37.0	37.0	33.0	37.0
15	35.61073989293776	37.0	37.0	37.0	33.0	37.0
16	35.60401015238539	37.0	37.0	37.0	33.0	37.0
17	35.59321128954819	37.0	37.0	37.0	33.0	37.0
18	35.57644770107042	37.0	37.0	37.0	33.0	37.0
19	35.56367454193736	37.0	37.0	37.0	33.0	37.0
20	35.56585005309283	37.0	37.0	37.0	33.0	37.0
21	35.57088042458328	37.0	37.0	37.0	33.0	37.0
22	35.48464292331323	37.0	37.0	37.0	33.0	37.0
23	35.53326021862692	37.0	37.0	37.0	33.0	37.0
24	35.51916720954834	37.0	37.0	37.0	33.0	37.0
25	35.54121318614765	37.0	37.0	37.0	33.0	37.0
26	35.42858665487269	37.0	37.0	37.0	33.0	37.0
27	35.44821606093025	37.0	37.0	37.0	33.0	37.0
28	35.47188271365133	37.0	37.0	37.0	33.0	37.0
29	35.48045924801425	37.0	37.0	37.0	33.0	37.0
30	35.469458174204256	37.0	37.0	37.0	33.0	37.0
31	35.46712029660266	37.0	37.0	37.0	33.0	37.0
32	35.45603455317352	37.0	37.0	37.0	33.0	37.0
33	35.461471338835864	37.0	37.0	37.0	33.0	37.0
34	35.43501756147512	37.0	37.0	37.0	33.0	37.0
35	35.38348364681015	37.0	37.0	37.0	33.0	37.0
36	35.404966819470424	37.0	37.0	37.0	33.0	37.0
37	35.406789706564986	37.0	37.0	37.0	33.0	37.0
38	35.394890138678875	37.0	37.0	37.0	33.0	37.0
39	35.29350474448701	37.0	37.0	37.0	33.0	37.0
40	35.09688395879279	37.0	37.0	37.0	33.0	37.0
41	35.2565061131465	37.0	37.0	37.0	33.0	37.0
42	35.299530233030715	37.0	37.0	37.0	33.0	37.0
43	34.955926972951545	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	24.0
16	47.0
17	44.0
18	36.0
19	32.0
20	65.0
21	187.0
22	488.0
23	1092.0
24	2379.0
25	4253.0
26	6897.0
27	10069.0
28	14226.0
29	18964.0
30	24699.0
31	31514.0
32	40025.0
33	52617.0
34	75925.0
35	153899.0
36	566417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63135445491692	20.366629412034243	13.981045958669272	24.02097017437957
2	15.846367474115999	21.84506057364165	39.58742984873025	22.721142103512097
3	17.791079208926767	27.338027018573523	30.98977788668615	23.881115885813557
4	12.676735378552886	17.98014148791416	39.30652593579851	30.036597197734437
5	13.395630250761528	37.6465033439519	35.553968415243716	13.403897990042854
6	28.904713806726157	39.88457040627472	17.628812374116198	13.581903412882932
7	25.753111359475326	32.84583554968513	23.64822462750348	17.752828463336062
8	23.935902109966907	36.49041440299949	20.709192729967665	18.86449075706593
9	25.69244806764007	16.04837922426691	20.041298851879965	38.21787385621306
10	14.758512285063683	28.632974134925522	33.94315381381848	22.665359766192317
11	33.00112959233073	24.199274431169577	24.35456847381517	18.445027502684525
12	22.37190482736363	26.367912405792598	30.024345005787417	21.23583776105636
13	27.272681994856075	22.705005070216018	27.03749967626322	22.98481325866469
14	21.82643325742951	21.76288123741162	27.76426384248662	28.64642166267225
15	23.81497397156296	28.75191004699662	25.231148060268833	22.20196792117159
16	22.75222083430454	29.09258074991384	24.963392841133896	23.191805574647724
17	22.335745919422415	27.991776089698	26.178451681538633	23.494026309340953
18	22.312237648694794	27.755298823988795	27.991178421798146	21.94128510551827
19	24.887190183902412	26.629790557245624	27.95741018545635	20.52560907339561
20	24.361043209396932	25.95442583040974	28.46811740588225	21.216413554311078
21	22.200872196688522	27.784285717131752	27.397395363292432	22.617446722887294
22	21.997764722054544	27.762172004837126	27.311331185713346	22.928732087394984
23	22.03900380714452	27.54382399875685	28.082521999159283	22.334650194939346
24	23.1762662092515	27.873039400260186	26.733884383137003	22.216810007351313
25	23.164711296520977	26.903721678012392	27.788967449013946	22.14259957645268
26	22.92006590284709	27.128843253624357	27.574803118232655	22.376287725295896
27	22.727118782510644	27.043376743945124	27.233136302149013	22.996368171395215
28	21.771945867226083	27.348984263404198	28.183727096868022	22.695342772501697
29	23.3916258758325	27.4988992949511	26.986598293458925	22.122876535757474
30	22.44810748459511	27.625106833137096	27.602196230309335	22.324589451958456
31	22.90701682036693	27.13213042707356	27.705094720400997	22.255758032158518
32	21.664465256568867	27.19926845449058	27.9805200109174	23.155746278023155
33	21.947560618466746	26.967672143296856	28.44351341067156	22.641253827564842
34	22.111620456976876	27.038894234696215	27.93738831081121	22.912096997515693
35	22.51215756119621	27.734081613543953	27.4123370607888	22.34142376447103
36	22.93301537401061	27.549003787222258	27.20584280138898	22.31213803737815
37	23.310542264085537	26.45009174202263	27.243595490396473	22.99577050349536
38	23.284842544391786	27.12774752914129	27.282045458620463	22.305364467846463
39	22.657888917444133	26.99576253459003	27.701508713001864	22.64483983496397
40	22.604198417773848	27.26072863685898	27.750417869473313	22.38465507589386
41	22.203561702237867	27.01877274873444	27.921649722781705	22.856015826245986
42	22.48864929046859	27.193789832075243	27.495512510185254	22.82204836727091
43	22.57630724911396	26.63058744777877	27.633673406368352	23.159431896738923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	46.5
2	65.0
3	267.0
4	469.0
5	469.0
6	551.5
7	634.0
8	597.0
9	560.0
10	845.0
11	1130.0
12	1130.0
13	1698.0
14	2266.0
15	4397.5
16	6529.0
17	7409.5
18	8290.0
19	8290.0
20	8091.5
21	7893.0
22	9245.5
23	10598.0
24	12930.5
25	15263.0
26	15263.0
27	18791.5
28	22320.0
29	28632.0
30	34944.0
31	38035.5
32	41127.0
33	41127.0
34	47397.0
35	53667.0
36	56342.0
37	59017.0
38	65937.0
39	72857.0
40	72857.0
41	77189.5
42	81522.0
43	77350.5
44	73179.0
45	74344.5
46	75510.0
47	75510.0
48	74502.5
49	73495.0
50	75816.0
51	78137.0
52	78025.0
53	77913.0
54	77913.0
55	67632.5
56	57352.0
57	50254.0
58	43156.0
59	37797.0
60	32438.0
61	32438.0
62	28766.0
63	25094.0
64	20804.5
65	16515.0
66	13965.0
67	11415.0
68	11415.0
69	9467.5
70	7520.0
71	6443.0
72	5366.0
73	4405.5
74	3445.0
75	3445.0
76	2575.5
77	1706.0
78	1334.0
79	962.0
80	761.5
81	561.0
82	561.0
83	470.5
84	380.0
85	344.0
86	308.0
87	238.5
88	169.0
89	169.0
90	124.0
91	79.0
92	48.5
93	18.0
94	11.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1003902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.965004808772306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.79549186507533	38.51660186531628
2	9.001355319284723	8.27494681072781
3	2.4172042302303485	3.333204121989682
4	1.140456912797512	2.0968442992374103
5	0.6783390993665943	1.558992998218189
6	0.45575105767347546	1.2569159734538615
7	0.3182915685617963	1.0241191435674253
8	0.239962332033554	0.8823895796677219
9	0.19101221394714332	0.7901889599353219
>10	1.358744359595513	12.63665802157351
>50	0.2063205999041666	6.755656232207449
>100	0.17989558741209466	16.12931731853442
>500	0.013211426244409793	3.8498467161306484
>1k	0.003963427873322938	2.8943179594402477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4333	0.4316158350117841	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2937	0.2925584369789083	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1902	0.18946072425396104	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.17252680042474264	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1724	0.17172990989160297	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1660	0.16535478562648545	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1444	0.14383874123171386	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1358	0.13527216800046218	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1327	0.1321842171845459	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1310	0.13049082480162405	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1206	0.12013124787080812	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1162	0.11574834993853982	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1138	0.11335767833912075	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1116	0.11116622937298659	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1099	0.10947283699006477	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1057	0.10528916169108139	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1054	0.10499032774115402	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1038	0.10339654667487463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.9922263328492223E-4	9.961131664246112E-5	0.0
3	0.0	0.0	1.9922263328492223E-4	9.961131664246112E-5	0.0
4	0.0	0.0	3.9844526656984447E-4	1.9922263328492223E-4	0.0
5	0.0	0.0	5.976678998547667E-4	1.9922263328492223E-4	0.0
6	0.0	0.0	8.965018497821501E-4	2.9883394992738337E-4	0.0
7	0.0	0.0	0.0020918376494916836	2.9883394992738337E-4	0.0
8	9.961131664246112E-5	0.0	0.0024902829160615278	2.9883394992738337E-4	0.0
9	9.961131664246112E-5	0.0	0.0026895055493464503	9.961131664246111E-4	0.0
10	3.9844526656984447E-4	0.0	0.0030879508159162945	0.0010957244830670722	0.0
11	3.9844526656984447E-4	0.0	0.005179788465407978	0.0014941697496369168	0.0
12	3.9844526656984447E-4	0.0	0.005478622415335361	0.0015937810662793779	0.0
13	3.9844526656984447E-4	0.0	0.0063751242651175115	0.001693392382921839	0.0
14	3.9844526656984447E-4	0.0	0.006673958215044895	0.001693392382921839	0.0
15	3.9844526656984447E-4	0.0	0.007172014798257201	0.0017930036995643002	0.0
16	3.9844526656984447E-4	0.0	0.0073712374315421225	0.0019922263328492223	0.0
17	3.9844526656984447E-4	0.0	0.007470848748184584	0.0020918376494916836	0.0
18	3.9844526656984447E-4	0.0	0.008068516648039351	0.0021914489661341444	0.0
19	3.9844526656984447E-4	0.0	0.00816812796468181	0.0021914489661341444	0.0
20	3.9844526656984447E-4	0.0	0.008267739281324273	0.0021914489661341444	0.0
21	3.9844526656984447E-4	0.0	0.008765795864536578	0.002390671599419067	0.0
22	3.9844526656984447E-4	0.0	0.00886540718117904	0.002589894232703989	0.0
23	3.9844526656984447E-4	0.0	0.008965018497821501	0.0030879508159162945	0.0
24	3.9844526656984447E-4	0.0	0.009064629814463961	0.0038848413490559838	0.0
25	3.9844526656984447E-4	0.0	0.009164241131106423	0.004283286615625828	0.0
26	3.9844526656984447E-4	0.0	0.009164241131106423	0.004880954515480595	0.0
27	3.9844526656984447E-4	0.0	0.009164241131106423	0.005578233731977822	0.0
28	3.9844526656984447E-4	0.0	0.009164241131106423	0.010758022197385801	0.0
29	3.9844526656984447E-4	0.0	0.009164241131106423	0.02410593862747559	0.0
30	3.9844526656984447E-4	0.0	0.009164241131106423	0.04293247747290074	0.0
31	3.9844526656984447E-4	0.0	0.009164241131106423	0.0950291960769079	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTA	25	0.0054960162	29.6	28
GGTATCA	995	0.0	26.773869	1
TTCGCCG	135	0.0	23.296295	24
CGATCGC	40	0.0019310174	23.125002	10
CGCTCTC	145	0.0	21.689655	29
GTATCAA	2515	0.0	21.626242	1
CCGCTCT	150	0.0	20.966667	28
GGACCGT	115	1.3460522E-10	20.913044	6
TGCGTTT	80	6.9583075E-7	20.8125	24
ATACGCC	45	0.0038254762	20.555555	6
CGATCCT	45	0.0038254762	20.555555	33
AATACGC	45	0.0038254762	20.555555	5
CCGGCAG	175	0.0	20.085714	16
AGCTTCG	195	0.0	19.923075	21
GCGTTTA	75	9.263853E-6	19.733334	25
GCCGGCA	190	0.0	19.473684	15
GCTTCGC	195	0.0	18.974358	22
TCGGGTA	50	0.0070342766	18.5	25
AACGGCC	210	0.0	18.5	37
ACGTGCA	50	0.0070342766	18.5	9
>>END_MODULE