FastQCFastQC Report
Fri 10 Feb 2017
ERR1633163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510898
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23340.45684265743847113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.3233522151192606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10420.20395460542026No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5160.10099863377817098No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT5150.10080289999177917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT405.933769E-527.7515
GGTATCA4000.026.3625011
GTATCAA11700.025.1410261
TCGTCTT901.4188117E-1024.66666614
TCTCGTC1103.6379788E-1223.54545412
TGGGGCG400.001929726623.12529
AACGGCC855.1664756E-821.76470637
TTAACGG855.1664756E-821.76470635
TAACGGC909.4523784E-820.55555536
GCAACTG555.13772E-420.18181831
CCCAATC759.249259E-619.73333217
TTCTCGT1153.0377123E-919.30434811
TGTCATG1355.638867E-1119.18518625
GTCATGC1103.839159E-818.526
TGCCCGC1103.839159E-818.530
GACGTAT801.613807E-518.526
AGGACCG609.227241E-418.55
GTCTAGA902.147457E-618.51
TAGGACC801.613807E-518.54
GTGACAC1002.8671639E-718.524
Produced by FastQC (version 0.11.5)