FastQCFastQC Report
Fri 10 Feb 2017
ERR1633162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947590
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41920.44238541985457847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.3101552359142667No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22330.23565043953608628No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19370.2044133011112401No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17370.18330712649985753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16970.179085891577581No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG16340.17243744657499552No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14940.15766312434702773No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA14930.1575575934739708No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13260.13993393767346637No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11720.12368218322270179No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11310.11935541742736837No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11290.11914435568125455No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10520.11101847845587226No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10300.10869679924862018No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10170.10732489789888032No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10000.1055308730569128No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9970.10521428043774206No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9770.10310366297660381No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9750.10289260123048999No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9520.100465391150181No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT9510.10035986027712408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.027.029941
GCCGGCA1850.026.015
CCGCTCT1750.024.31428728
CGCTCTC1750.024.31428729
GTATCAA23450.022.7995741
TTCGCCG1900.022.39473724
GCCGCTC2100.021.14285727
CCGGCAG2200.021.0227316
TCGAGTC555.142176E-420.1818231
GCCTAAG656.900494E-519.9230771
GCTTCGC2400.019.27083422
TGCGGGT1254.129106E-1019.24000221
TCTATAC1103.8495273E-818.5000023
TGCGGTA1409.458745E-1118.536
TGCTCGC2600.018.510
ACCGTGG500.00703399318.58
CCTTGCG1409.458745E-1118.533
ATCAAGC6400.018.21093830
AGCTTCG2450.018.1224521
TGCATCA6350.018.06299214
Produced by FastQC (version 0.11.5)