##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	947590
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838303485684737	33.0	33.0	33.0	27.0	33.0
2	31.95191591300035	33.0	33.0	33.0	33.0	33.0
3	31.680801823573486	33.0	33.0	33.0	27.0	33.0
4	31.967336084171425	33.0	33.0	33.0	33.0	33.0
5	32.05933262275879	33.0	33.0	33.0	33.0	33.0
6	35.2203231355333	37.0	37.0	37.0	33.0	37.0
7	35.36417860044956	37.0	37.0	37.0	33.0	37.0
8	35.49072172564084	37.0	37.0	37.0	33.0	37.0
9	35.61465929357634	37.0	37.0	37.0	33.0	37.0
10	35.611482814297325	37.0	37.0	37.0	33.0	37.0
11	35.62243164237698	37.0	37.0	37.0	33.0	37.0
12	35.613546998174314	37.0	37.0	37.0	33.0	37.0
13	35.620771641743794	37.0	37.0	37.0	33.0	37.0
14	35.60478160385821	37.0	37.0	37.0	33.0	37.0
15	35.61140472145126	37.0	37.0	37.0	33.0	37.0
16	35.611578847391804	37.0	37.0	37.0	33.0	37.0
17	35.59701980814487	37.0	37.0	37.0	33.0	37.0
18	35.58235734864235	37.0	37.0	37.0	33.0	37.0
19	35.58205025380175	37.0	37.0	37.0	33.0	37.0
20	35.58206291750652	37.0	37.0	37.0	33.0	37.0
21	35.5822592049304	37.0	37.0	37.0	33.0	37.0
22	35.48693316729809	37.0	37.0	37.0	33.0	37.0
23	35.52631517850547	37.0	37.0	37.0	33.0	37.0
24	35.52339513924799	37.0	37.0	37.0	33.0	37.0
25	35.542780105319814	37.0	37.0	37.0	33.0	37.0
26	35.43590687955761	37.0	37.0	37.0	33.0	37.0
27	35.44513133317152	37.0	37.0	37.0	33.0	37.0
28	35.482322523454236	37.0	37.0	37.0	33.0	37.0
29	35.48406378285968	37.0	37.0	37.0	33.0	37.0
30	35.473988750408935	37.0	37.0	37.0	33.0	37.0
31	35.458268871558374	37.0	37.0	37.0	33.0	37.0
32	35.46027606876392	37.0	37.0	37.0	33.0	37.0
33	35.46687280363871	37.0	37.0	37.0	33.0	37.0
34	35.44439156175139	37.0	37.0	37.0	33.0	37.0
35	35.38018974450976	37.0	37.0	37.0	33.0	37.0
36	35.41151869479416	37.0	37.0	37.0	33.0	37.0
37	35.40873479036292	37.0	37.0	37.0	33.0	37.0
38	35.39756645806731	37.0	37.0	37.0	33.0	37.0
39	35.30552137527834	37.0	37.0	37.0	33.0	37.0
40	35.10783777794194	37.0	37.0	37.0	33.0	37.0
41	35.27359828617862	37.0	37.0	37.0	33.0	37.0
42	35.30479954410663	37.0	37.0	37.0	33.0	37.0
43	34.97214829198282	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	36.0
16	46.0
17	37.0
18	33.0
19	32.0
20	55.0
21	151.0
22	433.0
23	1035.0
24	2134.0
25	3727.0
26	6155.0
27	9546.0
28	12925.0
29	17781.0
30	23153.0
31	29926.0
32	38291.0
33	50502.0
34	73457.0
35	147477.0
36	530655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.793075064110006	20.019734273261644	13.331293069787566	24.855897592840787
2	16.51832543610633	20.75992781688283	39.00906510199559	23.712681645015248
3	17.7780474677867	27.15689274897371	29.517723910129906	25.547335873109677
4	12.439240599837483	17.392015534144516	39.61660633818423	30.552137527833768
5	13.805232220686161	37.23688515075085	35.21164216591564	13.746240462647346
6	29.909665572663286	39.55318228347703	17.010732489789888	13.526419654069798
7	27.232347323209403	31.830327462299095	23.245602000865354	17.691723213626144
8	24.899376312540234	35.5139881172237	20.101837292499923	19.48479827773615
9	26.469359111007922	14.946865205415843	19.86734769256746	38.716427991008764
10	15.808208191306367	27.445097563292144	33.20718876307264	23.539505482328853
11	34.43831192815458	23.4593020188056	23.441678363005096	18.66070769003472
12	23.826338395297544	25.787102016694984	28.927595267995653	21.45896432001182
13	27.750081786426616	22.012895872687555	25.83469644044365	24.40232590044217
14	22.189237961565656	20.531559007587667	27.588725081522597	29.690477949324073
15	24.666575206576685	27.80537996390844	24.714802815563694	22.813242013951182
16	23.25056195189903	27.763167614685674	24.69950083897044	24.286769594444856
17	22.73736531622326	27.076372692831285	25.695395688008528	24.490866302936926
18	23.344062305427453	26.586920503593326	27.567724437784275	22.501292753194946
19	25.59556348209669	25.518103821272913	26.769277852235675	22.11705484439473
20	24.50131385936956	25.816439599404806	27.345054295634185	22.337192245591446
21	23.785603478297578	26.498274570225522	27.12491689443747	22.59120505703944
22	23.369600776707227	26.509144250150378	26.844732426471364	23.27652254667103
23	23.224706888000085	26.439704935678932	27.617429478994083	22.718158697326903
24	23.66614252999715	27.02941145432096	25.792589622093942	23.511856393587944
25	23.38891292647664	26.58808134319695	27.44414778543463	22.578857944891777
26	23.73146614041938	26.641374434090693	27.363311136673037	22.26384828881689
27	23.714370138984158	26.508300003165925	26.134404119925286	23.64292573792463
28	22.639221604280333	26.16363617176205	27.80654080351207	23.39060142044555
29	24.77843793201701	25.9185934845239	26.528984054285083	22.77398452917401
30	23.925854008590214	25.666058105298706	27.47622917084393	22.93185871526715
31	23.410546755453307	26.174505851686913	27.306957650460646	23.107989742399138
32	22.24432507730136	25.924503213415086	27.693728300214225	24.137443409069324
33	22.5295750271742	26.26895598307285	27.92368007260524	23.277788917147713
34	23.399149421163163	26.189913359153223	27.673782965206474	22.737154254477147
35	23.491488935087958	26.70310999482899	26.925569075232957	22.879831994850093
36	24.341645648434447	26.399181080425077	26.30103736848215	22.958135902658324
37	23.28570373262698	25.898120495150856	26.83016916598951	23.986006606232653
38	23.867706497535853	26.259141611878555	26.640846779725408	23.23230511086018
39	22.688926645490138	26.42471955170485	27.256724954885552	23.62962884791946
40	23.239164617608882	26.84082778416826	27.40752857248388	22.51247902573898
41	22.867062759210206	25.702466256503342	27.56086493103557	23.869606053250877
42	22.526725693601662	26.99078715478213	27.120273536022964	23.36221361559324
43	23.758587574795005	25.22420033980941	27.167340305406345	23.849871779989236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	45.5
2	64.0
3	243.0
4	422.0
5	422.0
6	520.5
7	619.0
8	582.5
9	546.0
10	722.5
11	899.0
12	899.0
13	1498.5
14	2098.0
15	3676.0
16	5254.0
17	5962.5
18	6671.0
19	6671.0
20	6097.5
21	5524.0
22	5740.5
23	5957.0
24	7798.0
25	9639.0
26	9639.0
27	12708.5
28	15778.0
29	19010.5
30	22243.0
31	27099.5
32	31956.0
33	31956.0
34	40280.5
35	48605.0
36	53391.0
37	58177.0
38	63032.5
39	67888.0
40	67888.0
41	71263.5
42	74639.0
43	76678.5
44	78718.0
45	76429.5
46	74141.0
47	74141.0
48	70491.5
49	66842.0
50	67977.0
51	69112.0
52	66141.0
53	63170.0
54	63170.0
55	63028.0
56	62886.0
57	54180.5
58	45475.0
59	41375.0
60	37275.0
61	37275.0
62	33760.0
63	30245.0
64	24293.0
65	18341.0
66	15482.0
67	12623.0
68	12623.0
69	10768.0
70	8913.0
71	7728.5
72	6544.0
73	6871.0
74	7198.0
75	7198.0
76	6135.0
77	5072.0
78	3786.0
79	2500.0
80	1573.5
81	647.0
82	647.0
83	511.0
84	375.0
85	319.5
86	264.0
87	199.5
88	135.0
89	135.0
90	110.0
91	85.0
92	52.0
93	19.0
94	11.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	947590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.124633891518265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68516429082023	39.907573632479796
2	8.581528561002848	8.088027833337335
3	2.3100013713053835	3.26573906735014
4	1.0181646336299868	1.9192254240441982
5	0.5976532354931994	1.4082094958349185
6	0.400658109471708	1.1328520034713252
7	0.30431877110427386	1.0038637472223957
8	0.22252622190829194	0.8389173390952807
9	0.16876168890956647	0.7157549524299858
>10	1.337932501835976	12.823639371928541
>50	0.19052008032122386	6.31372024343933
>100	0.16613431350506716	15.331130227614084
>500	0.012989925746050957	4.328835195597374
>1k	0.0036462949462599177	2.9225114661553864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.44238541985457847	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2939	0.3101552359142667	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2233	0.23565043953608628	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1937	0.2044133011112401	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1737	0.18330712649985753	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.179085891577581	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1634	0.17243744657499552	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1494	0.15766312434702773	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1493	0.1575575934739708	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1326	0.13993393767346637	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1172	0.12368218322270179	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1131	0.11935541742736837	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1129	0.11914435568125455	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1052	0.11101847845587226	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1030	0.10869679924862018	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	1017	0.10732489789888032	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1000	0.1055308730569128	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	997	0.10521428043774206	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	977	0.10310366297660381	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	975	0.10289260123048999	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	952	0.100465391150181	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	951	0.10035986027712408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.055308730569128E-4	0.0
4	0.0	0.0	2.110617461138256E-4	3.165926191707384E-4	0.0
5	0.0	0.0	4.221234922276512E-4	4.221234922276512E-4	0.0
6	0.0	0.0	9.497778575122152E-4	4.221234922276512E-4	0.0
7	0.0	0.0	0.0025327409533659074	5.27654365284564E-4	0.0
8	0.0	0.0	0.003165926191707384	6.331852383414768E-4	0.0
9	0.0	0.0	0.004115704049219599	0.0022161483341951688	0.0
10	0.0	0.0	0.0051710127797887275	0.0024272100803089942	0.0
11	0.0	0.0	0.008548000717609937	0.0024272100803089942	0.0
12	0.0	0.0	0.009286716829008326	0.0024272100803089942	0.0
13	0.0	0.0	0.01013096381346363	0.0024272100803089942	0.0
14	1.055308730569128E-4	0.0	0.010447556432634368	0.0024272100803089942	0.0
15	1.055308730569128E-4	0.0	0.011080741670975844	0.0025327409533659074	0.0
16	1.055308730569128E-4	0.0	0.01245264302071571	0.0025327409533659074	0.0
17	1.055308730569128E-4	0.0	0.012874766512943362	0.0028493335725366456	0.0
18	1.055308730569128E-4	0.0	0.012980297386000275	0.0028493335725366456	0.0
19	1.055308730569128E-4	0.0	0.012980297386000275	0.0028493335725366456	0.0
20	1.055308730569128E-4	0.0	0.012980297386000275	0.003060395318650471	0.0
21	1.055308730569128E-4	0.0	0.013402420878227926	0.0033769879378212097	0.0
22	1.055308730569128E-4	0.0	0.013719013497398664	0.004115704049219599	0.0
23	1.055308730569128E-4	0.0	0.01393007524351249	0.00453782754144725	0.0
24	2.110617461138256E-4	0.0	0.014035606116569403	0.004748889287561076	0.0
25	2.110617461138256E-4	0.0	0.014035606116569403	0.005065481906731815	0.0
26	2.110617461138256E-4	0.0	0.014035606116569403	0.006226321510357855	0.0
27	2.110617461138256E-4	0.0	0.014035606116569403	0.007387161113983896	0.0
28	2.110617461138256E-4	0.0	0.014035606116569403	0.015301976593252355	0.0
29	2.110617461138256E-4	0.0	0.014035606116569403	0.031237138424846187	0.0
30	2.110617461138256E-4	0.0	0.014035606116569403	0.05181565867094418	0.0
31	2.110617461138256E-4	0.0	0.014035606116569403	0.10669171266053884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	27.02994	1
GCCGGCA	185	0.0	26.0	15
CCGCTCT	175	0.0	24.314287	28
CGCTCTC	175	0.0	24.314287	29
GTATCAA	2345	0.0	22.799574	1
TTCGCCG	190	0.0	22.394737	24
GCCGCTC	210	0.0	21.142857	27
CCGGCAG	220	0.0	21.02273	16
TCGAGTC	55	5.142176E-4	20.18182	31
GCCTAAG	65	6.900494E-5	19.923077	1
GCTTCGC	240	0.0	19.270834	22
TGCGGGT	125	4.129106E-10	19.240002	21
TCTATAC	110	3.8495273E-8	18.500002	3
TGCGGTA	140	9.458745E-11	18.5	36
TGCTCGC	260	0.0	18.5	10
ACCGTGG	50	0.007033993	18.5	8
CCTTGCG	140	9.458745E-11	18.5	33
ATCAAGC	640	0.0	18.210938	30
AGCTTCG	245	0.0	18.12245	21
TGCATCA	635	0.0	18.062992	14
>>END_MODULE