FastQCFastQC Report
Fri 10 Feb 2017
ERR1633161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814219
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47350.5815388734480527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.4061560833141943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19000.2333524518587751No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG13940.1712070094163855No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA12520.1537669840669402No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG12050.14799458131043366No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG10350.1271156777230696No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10010.12293989700559677No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9970.1224486286858941No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9940.12208017744611709No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9930.12195736036619141No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9270.11385143309109712No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT8900.10930720113384727No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8760.10758776201488789No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC8500.10439451793682045No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8400.10316634713756373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.101201273858753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT251.2333097E-436.9999964
CGAATGA250.005495414629.59999830
AACGAAG250.005495414629.59999828
TTCGAAC358.8671636E-426.4285722
GCGCACG659.384894E-825.61538534
GGTATCA8000.024.7437481
TTACTCG601.3366061E-624.66666819
TACTCGC451.3227492E-424.66666620
GTATCAA23600.024.3008481
AGCGCAC701.9214895E-723.78571533
TCGAACG400.001930706323.1253
ACTCGCA603.7245685E-521.58333421
GGACCGT806.954997E-720.81256
ATCCGTA555.1413226E-420.18181812
TTAACGG2200.020.18181835
GCTCTAG656.898973E-519.9230771
AAAGCGC851.2443961E-619.58823631
TTCGCCG1457.2759576E-1219.1379324
GCTTCGC1551.8189894E-1219.09677322
GTATATA1551.8189894E-1219.0967731
Produced by FastQC (version 0.11.5)