##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814219
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.856290506608172	33.0	33.0	33.0	33.0	33.0
2	31.958431331128356	33.0	33.0	33.0	33.0	33.0
3	31.690787122383536	33.0	33.0	33.0	27.0	33.0
4	31.97481758593204	33.0	33.0	33.0	33.0	33.0
5	32.07011135824637	33.0	33.0	33.0	33.0	33.0
6	35.22858714915766	37.0	37.0	37.0	33.0	37.0
7	35.37301635063785	37.0	37.0	37.0	33.0	37.0
8	35.50054469374947	37.0	37.0	37.0	33.0	37.0
9	35.618302938153	37.0	37.0	37.0	33.0	37.0
10	35.619138094296495	37.0	37.0	37.0	33.0	37.0
11	35.63646635610321	37.0	37.0	37.0	33.0	37.0
12	35.61050159723612	37.0	37.0	37.0	33.0	37.0
13	35.61955444419745	37.0	37.0	37.0	33.0	37.0
14	35.60700622314144	37.0	37.0	37.0	33.0	37.0
15	35.62205991262793	37.0	37.0	37.0	33.0	37.0
16	35.61776131483053	37.0	37.0	37.0	33.0	37.0
17	35.592042190123294	37.0	37.0	37.0	33.0	37.0
18	35.58374958088672	37.0	37.0	37.0	33.0	37.0
19	35.56996704817745	37.0	37.0	37.0	33.0	37.0
20	35.57899410355199	37.0	37.0	37.0	33.0	37.0
21	35.56551861354255	37.0	37.0	37.0	33.0	37.0
22	35.47502207637012	37.0	37.0	37.0	33.0	37.0
23	35.51857424108256	37.0	37.0	37.0	33.0	37.0
24	35.506809592996476	37.0	37.0	37.0	33.0	37.0
25	35.52856541053451	37.0	37.0	37.0	33.0	37.0
26	35.417436832105366	37.0	37.0	37.0	33.0	37.0
27	35.427109905320314	37.0	37.0	37.0	33.0	37.0
28	35.44941348703482	37.0	37.0	37.0	33.0	37.0
29	35.44931277702928	37.0	37.0	37.0	33.0	37.0
30	35.44191550430535	37.0	37.0	37.0	33.0	37.0
31	35.44393830161173	37.0	37.0	37.0	33.0	37.0
32	35.43772621370909	37.0	37.0	37.0	33.0	37.0
33	35.4358876420226	37.0	37.0	37.0	33.0	37.0
34	35.41613988374135	37.0	37.0	37.0	33.0	37.0
35	35.35383600726586	37.0	37.0	37.0	33.0	37.0
36	35.38374319439856	37.0	37.0	37.0	33.0	37.0
37	35.369561506179544	37.0	37.0	37.0	33.0	37.0
38	35.35879167644086	37.0	37.0	37.0	33.0	37.0
39	35.25863680410307	37.0	37.0	37.0	33.0	37.0
40	35.04864538901696	37.0	37.0	37.0	27.0	37.0
41	35.221616051701076	37.0	37.0	37.0	33.0	37.0
42	35.25059105719714	37.0	37.0	37.0	33.0	37.0
43	34.9211796826161	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	21.0
16	35.0
17	40.0
18	37.0
19	26.0
20	60.0
21	147.0
22	399.0
23	887.0
24	1913.0
25	3305.0
26	5373.0
27	8040.0
28	11729.0
29	15607.0
30	20144.0
31	25813.0
32	33198.0
33	43205.0
34	62571.0
35	126667.0
36	454999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93120155633804	20.26457255357588	13.288193962557985	23.516031927528097
2	16.633117134333638	21.892021679670947	38.92159234800465	22.55326883799076
3	18.2916389816499	27.301377147917204	29.461483949649907	24.945499920782986
4	12.106816470752953	17.76929794072602	39.96516907613308	30.158716512387944
5	14.196671902768173	36.499516714790495	35.126053309981714	14.17775807245962
6	29.399092873047667	40.19102968611639	16.995181885954516	13.414695554881426
7	27.656686960142174	31.762339124977434	22.79497285128448	17.78600106359591
8	24.794434912474408	35.228728388799574	20.461079881456953	19.515756817269065
9	26.35642253496909	14.943645382876106	20.18449581746434	38.51543626469046
10	15.442773995694035	27.810208310049262	33.45070552271564	23.29631217154107
11	34.34984936485147	24.13269648583489	23.218568959948122	18.298885189365517
12	23.46911580299649	25.592500297831418	29.87230708200128	21.06607681717081
13	28.478824493164616	22.500089042382946	25.94105517066047	23.080031293791965
14	22.681981137752867	21.036723535068575	27.734061720495344	28.547233606683214
15	24.019950406463124	28.580025767023372	25.199362824989347	22.20066100152416
16	23.394688652561534	28.16269332943594	24.859896416074793	23.582721601927737
17	23.113314722451822	26.865376514181072	25.66852407030541	24.352784693061693
18	23.282065390269693	26.18644369635196	28.137515828051175	22.393975085327167
19	26.03906320044116	25.66962942402474	27.324589576023158	20.966717799510942
20	25.276614768262597	25.82880035960841	27.42714183776109	21.467443034367903
21	23.308471062453716	26.79561641278329	27.42161506916444	22.474297455598556
22	23.478695535230692	26.04151954203967	27.57599613863101	22.903788784098627
23	22.601413133321625	26.411444586775794	28.400221562012185	22.586920717890397
24	23.04588814557263	27.242056498313104	26.619619537249804	23.092435818864455
25	23.200883300438825	26.95368199464763	27.984117295224014	21.861317409689534
26	24.088727971221502	26.418813611571334	27.039531133515677	22.452927283691487
27	23.504118670775306	26.418199526171705	26.6549908562684	23.422690946784588
28	22.860802806124642	26.181285378995085	28.082248142084627	22.87566367279565
29	24.260917517277292	26.629567720723784	27.132626480099336	21.976888281899587
30	23.885220069784665	26.108946118918862	27.31107969723133	22.694754114065134
31	23.28083721947044	25.75560137997271	27.619473384924692	23.34408801563216
32	22.19808184284572	26.15058110901367	27.910795498508385	23.740541549632223
33	22.600799047921996	25.929633182227384	28.355147693679466	23.114420076171154
34	23.200269215039196	26.12098219275158	28.04773654262551	22.631012049583713
35	23.34752689387008	26.941154652495214	26.842409720234972	22.86890873339974
36	23.510136707691665	26.29661061704529	26.81465306017177	23.37859961509127
37	23.503258951215827	25.916123303435562	27.56039836948045	23.020219375868166
38	23.250869851968574	26.140510108459765	26.896572052482192	23.71204798708947
39	22.911280625974094	26.06497760430548	27.74536089184851	23.278380877871925
40	23.280468768230662	26.967191873439454	27.34927580908822	22.40306354924167
41	23.222744740665597	25.879769447777562	27.52257070886334	23.374915102693503
42	22.224119063789963	27.801856748614316	27.048128329110472	22.925895858485248
43	23.826759139740044	25.67073477774407	26.913520809511937	23.588985273003946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	49.5
2	67.0
3	279.0
4	491.0
5	491.0
6	588.5
7	686.0
8	637.5
9	589.0
10	808.5
11	1028.0
12	1028.0
13	1612.5
14	2197.0
15	4071.5
16	5946.0
17	6729.0
18	7512.0
19	7512.0
20	6789.0
21	6066.0
22	6414.5
23	6763.0
24	8577.5
25	10392.0
26	10392.0
27	12703.0
28	15014.0
29	17994.5
30	20975.0
31	24483.0
32	27991.0
33	27991.0
34	35580.0
35	43169.0
36	46666.5
37	50164.0
38	52580.5
39	54997.0
40	54997.0
41	56308.0
42	57619.0
43	59751.5
44	61884.0
45	60721.5
46	59559.0
47	59559.0
48	58174.0
49	56789.0
50	57755.0
51	58721.0
52	59361.5
53	60002.0
54	60002.0
55	58313.5
56	56625.0
57	48197.0
58	39769.0
59	36371.5
60	32974.0
61	32974.0
62	30183.5
63	27393.0
64	21110.0
65	14827.0
66	12563.5
67	10300.0
68	10300.0
69	8772.5
70	7245.0
71	7171.5
72	7098.0
73	6464.5
74	5831.0
75	5831.0
76	3639.0
77	1447.0
78	1179.5
79	912.0
80	691.0
81	470.0
82	470.0
83	379.5
84	289.0
85	246.5
86	204.0
87	158.5
88	113.0
89	113.0
90	79.0
91	45.0
92	33.0
93	21.0
94	12.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	814219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.6500403012157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32542938574855	42.860610480221645
2	7.715371005028465	7.661369626769887
3	1.8228092273069116	2.71507654791648
4	0.8863977603521206	1.7603873809756045
5	0.5580163183019835	1.385276634621474
6	0.35885443927063554	1.0690282423234327
7	0.2788042747235465	0.9689850433322709
8	0.19192713325629956	0.7623351920857655
9	0.16684283896849653	0.7455378310879582
>10	1.3404179958785045	13.361431752448965
>50	0.19052676117036996	6.577399700536819
>100	0.15227022981113048	14.625970017608353
>500	0.010319139540892265	3.528562283832562
>1k	0.0020134906421253197	1.9780292662388221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4735	0.5815388734480527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3307	0.4061560833141943	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1900	0.2333524518587751	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1394	0.1712070094163855	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1252	0.1537669840669402	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1205	0.14799458131043366	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1035	0.1271156777230696	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1001	0.12293989700559677	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	997	0.1224486286858941	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	994	0.12208017744611709	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	993	0.12195736036619141	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	927	0.11385143309109712	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	890	0.10930720113384727	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	876	0.10758776201488789	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	850	0.10439451793682045	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	840	0.10316634713756373	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.101201273858753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.2281707992567112E-4	0.0	0.0
2	0.0	0.0	1.2281707992567112E-4	0.0	0.0
3	0.0	0.0	2.4563415985134223E-4	0.0	0.0
4	0.0	0.0	3.684512397770133E-4	0.0	0.0
5	0.0	0.0	6.140853996283555E-4	1.2281707992567112E-4	0.0
6	0.0	0.0	9.82536639405369E-4	1.2281707992567112E-4	0.0
7	0.0	0.0	0.002087890358736409	1.2281707992567112E-4	0.0
8	0.0	0.0	0.002333524518587751	1.2281707992567112E-4	0.0
9	0.0	0.0	0.003561695317844462	0.0017194391189593954	0.0
10	2.4563415985134223E-4	0.0	0.004052963637547147	0.0018422561988850666	0.0
11	2.4563415985134223E-4	0.0	0.006509305236060569	0.0018422561988850666	0.0
12	2.4563415985134223E-4	0.0	0.006509305236060569	0.0018422561988850666	0.0
13	2.4563415985134223E-4	0.0	0.007246207715614595	0.002087890358736409	0.0
14	2.4563415985134223E-4	0.0	0.007860293115242951	0.002087890358736409	0.0
15	2.4563415985134223E-4	0.0	0.008351561434945635	0.002087890358736409	0.0
16	2.4563415985134223E-4	0.0	0.009702549314128017	0.00221070743866208	0.0
17	2.4563415985134223E-4	0.0	0.009825366394053688	0.00221070743866208	0.0
18	2.4563415985134223E-4	0.0	0.010071000553905031	0.002333524518587751	0.0
19	2.4563415985134223E-4	0.0	0.010316634713756373	0.002456341598513422	0.0
20	2.4563415985134223E-4	0.0	0.010439451793682044	0.0027019757583647643	0.0
21	2.4563415985134223E-4	0.0	0.010807903033459057	0.003070426998141778	0.0
22	2.4563415985134223E-4	0.0	0.0110535371933104	0.00331606115799312	0.0
23	3.684512397770133E-4	0.0	0.0110535371933104	0.0036845123977701333	0.0
24	3.684512397770133E-4	0.0	0.0110535371933104	0.004052963637547147	0.0
25	3.684512397770133E-4	0.0	0.0110535371933104	0.004789866117101173	0.0
26	3.684512397770133E-4	0.0	0.0110535371933104	0.0055267685966552	0.0
27	4.912683197026845E-4	0.0	0.0110535371933104	0.006509305236060569	0.0
28	4.912683197026845E-4	0.0	0.0110535371933104	0.014983683750931875	0.0
29	6.140853996283555E-4	0.0	0.0110535371933104	0.03119553830112046	0.0
30	6.140853996283555E-4	0.0	0.0110535371933104	0.05170599064870753	0.0
31	6.140853996283555E-4	0.0	0.0110535371933104	0.10783339617473923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	25	1.2333097E-4	36.999996	4
CGAATGA	25	0.0054954146	29.599998	30
AACGAAG	25	0.0054954146	29.599998	28
TTCGAAC	35	8.8671636E-4	26.428572	2
GCGCACG	65	9.384894E-8	25.615385	34
GGTATCA	800	0.0	24.743748	1
TTACTCG	60	1.3366061E-6	24.666668	19
TACTCGC	45	1.3227492E-4	24.666666	20
GTATCAA	2360	0.0	24.300848	1
AGCGCAC	70	1.9214895E-7	23.785715	33
TCGAACG	40	0.0019307063	23.125	3
ACTCGCA	60	3.7245685E-5	21.583334	21
GGACCGT	80	6.954997E-7	20.8125	6
ATCCGTA	55	5.1413226E-4	20.181818	12
TTAACGG	220	0.0	20.181818	35
GCTCTAG	65	6.898973E-5	19.923077	1
AAAGCGC	85	1.2443961E-6	19.588236	31
TTCGCCG	145	7.2759576E-12	19.13793	24
GCTTCGC	155	1.8189894E-12	19.096773	22
GTATATA	155	1.8189894E-12	19.096773	1
>>END_MODULE