FastQCFastQC Report
Fri 10 Feb 2017
ERR1633160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030147
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60350.5858387201049947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.40188439125678177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23920.23219987050391838No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14310.13891221349962674No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12250.11891506746124583No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11530.11192577370025832No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.10716917100180848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.025.0645161
TTCGCCG1750.024.31428724
GTATCAA35300.023.6359771
GTCGAAC400.00193105223.12516
GCGAAAC400.00193105223.12522
CGCTCTC2050.021.65853729
GCCGGCA2200.021.02272815
CCGCTCT2050.020.756128
CGACGAC450.003825542720.55555533
CGGTAAC450.003825542720.55555522
GCGATAG555.142596E-420.18181836
AGCTTCG2500.019.98000121
TCGTTGG1153.0486262E-919.30434822
GCCGCTC2250.018.91111227
TGCTCGC2550.018.86274510
GCTTCGC2500.018.50000222
GGTCGGA609.2359295E-418.51
GTCGTTG1205.171387E-918.521
GTATTAT1351.1514203E-917.8148161
CCGGCAG2650.017.45283116
Produced by FastQC (version 0.11.5)