FastQCFastQC Report
Fri 10 Feb 2017
ERR1633159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752937
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40240.5344404644744514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.3629785758967882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17590.23361848335252486No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11980.15911025756470992No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10490.139321085296645No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10460.13892264558654974No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10260.1362663808525813No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9820.1304225984378507No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9410.12497725573321539No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8180.10864122761930946No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7670.10186775254768991No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7650.10160212607429307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT250.005495153429.67
GGTATCA10650.023.6244131
CAACCGC400.001930571623.12500233
CCGGTCG400.001930571623.1250029
GTATCAA26500.021.7811321
TGCGGTA855.174661E-821.76470636
TACGGCA450.003824601220.55555515
ACTCGAC450.003824601220.55555527
GTACGGC555.140829E-420.18181814
GTCGCCC3150.018.79365237
TTTCGTC500.007032681718.517
GACCGTT500.007032681718.57
GTACTAT1103.846435E-818.51
TCTCCGA701.21848854E-418.511
AGGTCGC3200.018.535
AGCTTCG1451.546141E-1017.86206821
GCTCCGA3500.017.44285629
GCTCGGA3300.017.37878811
CATAATA2350.017.3191472
CTACGAC752.0660736E-417.2666663
Produced by FastQC (version 0.11.5)