##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752937
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83920434246159	33.0	33.0	33.0	27.0	33.0
2	31.959365790232116	33.0	33.0	33.0	33.0	33.0
3	31.682204487228017	33.0	33.0	33.0	27.0	33.0
4	31.96655231446987	33.0	33.0	33.0	33.0	33.0
5	32.06773342258383	33.0	33.0	33.0	33.0	33.0
6	35.22221646698196	37.0	37.0	37.0	33.0	37.0
7	35.36369311111022	37.0	37.0	37.0	33.0	37.0
8	35.4939178178254	37.0	37.0	37.0	33.0	37.0
9	35.61047471435193	37.0	37.0	37.0	33.0	37.0
10	35.60902173754245	37.0	37.0	37.0	33.0	37.0
11	35.61724818942355	37.0	37.0	37.0	33.0	37.0
12	35.61349621548682	37.0	37.0	37.0	33.0	37.0
13	35.609515802782965	37.0	37.0	37.0	33.0	37.0
14	35.59898504124515	37.0	37.0	37.0	33.0	37.0
15	35.61248683488791	37.0	37.0	37.0	33.0	37.0
16	35.59874730555146	37.0	37.0	37.0	33.0	37.0
17	35.598355506503204	37.0	37.0	37.0	33.0	37.0
18	35.57656882315519	37.0	37.0	37.0	33.0	37.0
19	35.5724051281847	37.0	37.0	37.0	33.0	37.0
20	35.557024027242655	37.0	37.0	37.0	33.0	37.0
21	35.56783635284227	37.0	37.0	37.0	33.0	37.0
22	35.470069873043826	37.0	37.0	37.0	33.0	37.0
23	35.51936616210918	37.0	37.0	37.0	33.0	37.0
24	35.51218495040089	37.0	37.0	37.0	33.0	37.0
25	35.53735305875525	37.0	37.0	37.0	33.0	37.0
26	35.42385219480514	37.0	37.0	37.0	33.0	37.0
27	35.43768734967202	37.0	37.0	37.0	33.0	37.0
28	35.4606374769735	37.0	37.0	37.0	33.0	37.0
29	35.46644805607906	37.0	37.0	37.0	33.0	37.0
30	35.47007385744093	37.0	37.0	37.0	33.0	37.0
31	35.44973085397583	37.0	37.0	37.0	33.0	37.0
32	35.45114929934377	37.0	37.0	37.0	33.0	37.0
33	35.44487121764504	37.0	37.0	37.0	33.0	37.0
34	35.41895138637097	37.0	37.0	37.0	33.0	37.0
35	35.357936985431714	37.0	37.0	37.0	33.0	37.0
36	35.38611065733255	37.0	37.0	37.0	33.0	37.0
37	35.37931726027543	37.0	37.0	37.0	33.0	37.0
38	35.354797280516166	37.0	37.0	37.0	33.0	37.0
39	35.26798922087771	37.0	37.0	37.0	33.0	37.0
40	35.05133098785157	37.0	37.0	37.0	33.0	37.0
41	35.23079620207268	37.0	37.0	37.0	33.0	37.0
42	35.256567282521644	37.0	37.0	37.0	33.0	37.0
43	34.91826407787106	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	27.0
16	33.0
17	18.0
18	32.0
19	28.0
20	43.0
21	103.0
22	346.0
23	860.0
24	1690.0
25	3113.0
26	5035.0
27	7439.0
28	10891.0
29	14551.0
30	18873.0
31	23995.0
32	30587.0
33	39748.0
34	57668.0
35	116008.0
36	421846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81571632155147	20.419503889435635	13.660107020906132	24.10467276810676
2	16.13680825885831	21.078655983169906	39.23781139723509	23.546724360736686
3	17.883435134679264	27.32964378161785	29.814048187298535	24.972872896404347
4	12.313646427257526	17.874802274293867	39.4817893130501	30.329761985398513
5	13.8816394997191	37.06658060368929	35.06986640316521	13.98191349342641
6	29.59225008201217	39.788322263350054	17.007133398943072	13.612294255694698
7	26.926289981764747	32.13814701628423	23.19835524087673	17.7372077610743
8	24.71986368049385	35.69807301274874	20.058251885615928	19.523811421141477
9	26.217864177215354	15.475663966573563	20.24312791110013	38.063343945110944
10	15.737306042869456	27.62980169655628	33.0580114936575	23.574880766916753
11	34.187986511487686	23.725490977332765	23.61472473792628	18.47179777325327
12	23.490278735139857	25.98265193502245	29.4723197292735	21.05474960056419
13	28.089202682296126	22.432421304836925	25.78847898296936	23.689897029897587
14	22.07927090845582	21.033366669455745	27.93102211738831	28.956340304700127
15	24.499128081101073	28.165039040450928	24.662621175476833	22.673211702971166
16	22.862869004976513	28.33729780844878	25.150975446816933	23.648857739757776
17	22.484351280386008	27.390472244025727	26.117191743797953	24.00798473179031
18	23.354277980760674	26.71285911039038	27.791701032091666	22.141161876757284
19	25.289499652693387	25.93058914623667	27.459003874162114	21.32090732690783
20	24.423955789129767	25.715166076311828	27.715731860700167	22.145146273858238
21	23.160503468417676	26.722421663432666	27.7800134672622	22.33706140088746
22	22.805892126432887	26.675538590878123	27.572692004775963	22.945877277913027
23	22.740680827213964	26.60049911214351	28.25415672227557	22.404663338366955
24	23.008299499161282	27.22618227022978	26.583366204609415	23.18215202599952
25	23.095690608908846	26.84075825733096	27.847748217978396	22.215802915781797
26	23.155589378659837	26.863070881096295	27.79860732039998	22.18273241984389
27	23.029151177322937	26.83132851752537	27.16721319313568	22.972307112016015
28	22.479835630338265	26.54763944393754	28.087608923455747	22.884916002268447
29	24.260595507990708	26.358114955168894	26.907430502153566	22.473859034686832
30	23.509403841224433	26.019972454534706	27.853592000393128	22.617031703847733
31	23.16010502870758	26.336997650533846	27.6535752659253	22.849322054833273
32	22.008879893005656	26.150926305919352	28.18004693619785	23.66014686487714
33	22.125224288353472	26.359575900772576	28.502783101375016	23.012416709498936
34	22.963275811920518	26.129809001284304	28.129843532725847	22.77707165406933
35	22.82913444285511	27.070126717109133	27.39857385146433	22.702164988571422
36	23.912359201367444	26.48428753003239	27.124712957392184	22.478640311207975
37	23.04721377751392	26.22131732136952	27.285948226744072	23.445520674372492
38	23.099675006009797	26.728531072320795	27.216088464240702	22.95570545742871
39	22.685164894274024	26.40977930424458	27.68518481625953	23.21987098522187
40	22.875353449226164	27.26881531920997	27.654239332108794	22.201591899455067
41	22.858486168165467	25.952237703818515	27.7968807483229	23.39239537969312
42	22.34449894214257	27.11873636174076	27.609879711051523	22.92688498506515
43	23.262504034202063	25.989425410094068	27.428855269431573	23.31921528627229
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	39.0
2	56.0
3	222.0
4	388.0
5	388.0
6	488.0
7	588.0
8	561.5
9	535.0
10	757.0
11	979.0
12	979.0
13	1515.5
14	2052.0
15	3609.5
16	5167.0
17	5956.0
18	6745.0
19	6745.0
20	6148.0
21	5551.0
22	5495.0
23	5439.0
24	7027.0
25	8615.0
26	8615.0
27	11208.0
28	13801.0
29	16364.0
30	18927.0
31	22885.5
32	26844.0
33	26844.0
34	33138.5
35	39433.0
36	42772.0
37	46111.0
38	49681.0
39	53251.0
40	53251.0
41	55534.0
42	57817.0
43	59242.5
44	60668.0
45	59908.0
46	59148.0
47	59148.0
48	57480.0
49	55812.0
50	55928.0
51	56044.0
52	53677.5
53	51311.0
54	51311.0
55	49431.0
56	47551.0
57	41365.0
58	35179.0
59	32506.0
60	29833.0
61	29833.0
62	26142.0
63	22451.0
64	18643.0
65	14835.0
66	12512.5
67	10190.0
68	10190.0
69	8504.5
70	6819.0
71	5602.0
72	4385.0
73	3581.0
74	2777.0
75	2777.0
76	2132.5
77	1488.0
78	1147.0
79	806.0
80	662.5
81	519.0
82	519.0
83	433.0
84	347.0
85	298.0
86	249.0
87	194.0
88	139.0
89	139.0
90	91.0
91	43.0
92	31.0
93	19.0
94	11.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	752937.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.85524325482487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5127159655608	47.763335516371455
2	8.335064087447531	9.311140642978733
3	2.298852992244483	3.8520897926669297
4	1.003139194030637	2.241223348041208
5	0.5840538545579372	1.6311235060125846
6	0.3795998354441562	1.2721584688934904
7	0.2637763861495349	1.0313305949282625
8	0.19032102502335377	0.8504341719349628
9	0.14635669146705363	0.7357309743477269
>10	1.0460674078637988	11.382997043691583
>50	0.13218552954329096	5.149044815228996
>100	0.09968069351870325	10.661049288847916
>500	0.006500914794599107	2.3922236416996276
>1k	0.001685422354155324	1.7261181943565729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4024	0.5344404644744514	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2733	0.3629785758967882	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.23361848335252486	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1198	0.15911025756470992	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1049	0.139321085296645	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1046	0.13892264558654974	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1026	0.1362663808525813	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	982	0.1304225984378507	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	941	0.12497725573321539	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	818	0.10864122761930946	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	767	0.10186775254768991	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	765	0.10160212607429307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.656264733968446E-4	0.0	0.0
2	0.0	0.0	2.656264733968446E-4	0.0	0.0
3	0.0	0.0	3.9843971009526693E-4	0.0	0.0
4	0.0	0.0	3.9843971009526693E-4	5.312529467936892E-4	0.0
5	0.0	0.0	6.640661834921115E-4	5.312529467936892E-4	0.0
6	0.0	0.0	0.001328132366984223	5.312529467936892E-4	0.0
7	0.0	0.0	0.002656264733968446	7.968794201905339E-4	0.0
8	1.328132366984223E-4	0.0	0.003054704444063713	7.968794201905339E-4	0.0
9	1.328132366984223E-4	0.0	0.004117210337651092	0.0015937588403810677	0.0
10	2.656264733968446E-4	0.0	0.004914089757841625	0.0015937588403810677	0.0
11	2.656264733968446E-4	0.0	0.0073047280184132275	0.001992198550476335	0.0
12	2.656264733968446E-4	0.0	0.008367233912000605	0.002125011787174757	0.0
13	2.656264733968446E-4	0.0	0.008765673622095873	0.002125011787174757	0.0
14	2.656264733968446E-4	0.0	0.00982817951568325	0.002125011787174757	0.0
15	2.656264733968446E-4	0.0	0.01035943246247694	0.002125011787174757	0.0
16	2.656264733968446E-4	0.0	0.011289125119365896	0.0022578250238731792	0.0
17	2.656264733968446E-4	0.0	0.011820378066159586	0.0022578250238731792	0.0
18	2.656264733968446E-4	0.0	0.011953191302858008	0.0023906382605716017	0.0
19	2.656264733968446E-4	0.0	0.012218817776254852	0.0023906382605716017	0.0
20	2.656264733968446E-4	0.0	0.012484444249651698	0.0023906382605716017	0.0
21	2.656264733968446E-4	0.0	0.01314851043314381	0.0027890779706668685	0.0
22	2.656264733968446E-4	0.0	0.01314851043314381	0.003851583864254247	0.0
23	2.656264733968446E-4	0.0	0.01314851043314381	0.004117210337651092	0.0
24	2.656264733968446E-4	0.0	0.01314851043314381	0.0046484632844447805	0.0
25	2.656264733968446E-4	0.0	0.013281323669842231	0.005312529467936892	1.328132366984223E-4
26	2.656264733968446E-4	0.0	0.013281323669842231	0.005445342704635315	1.328132366984223E-4
27	2.656264733968446E-4	0.0	0.013281323669842231	0.007171914781714805	1.328132366984223E-4
28	2.656264733968446E-4	0.0	0.013281323669842231	0.014476642800128031	1.328132366984223E-4
29	2.656264733968446E-4	0.0	0.013281323669842231	0.030945484150732398	1.328132366984223E-4
30	2.656264733968446E-4	0.0	0.013281323669842231	0.05604718588673421	1.328132366984223E-4
31	2.656264733968446E-4	0.0	0.013281323669842231	0.11222718501016685	1.328132366984223E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	0.0054951534	29.6	7
GGTATCA	1065	0.0	23.624413	1
CAACCGC	40	0.0019305716	23.125002	33
CCGGTCG	40	0.0019305716	23.125002	9
GTATCAA	2650	0.0	21.781132	1
TGCGGTA	85	5.174661E-8	21.764706	36
TACGGCA	45	0.0038246012	20.555555	15
ACTCGAC	45	0.0038246012	20.555555	27
GTACGGC	55	5.140829E-4	20.181818	14
GTCGCCC	315	0.0	18.793652	37
TTTCGTC	50	0.0070326817	18.5	17
GACCGTT	50	0.0070326817	18.5	7
GTACTAT	110	3.846435E-8	18.5	1
TCTCCGA	70	1.21848854E-4	18.5	11
AGGTCGC	320	0.0	18.5	35
AGCTTCG	145	1.546141E-10	17.862068	21
GCTCCGA	350	0.0	17.442856	29
GCTCGGA	330	0.0	17.378788	11
CATAATA	235	0.0	17.319147	2
CTACGAC	75	2.0660736E-4	17.266666	3
>>END_MODULE