FastQCFastQC Report
Fri 10 Feb 2017
ERR1633158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623058
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28440.4564583072522943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.2974040940008795No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC14260.2288711484324092No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13360.2144262652915138No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12180.19548741850678428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12110.19436392759582577No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12100.19420342889426026No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11840.19003046265355714No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9840.15793072234045627No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8520.13674489373380969No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7960.12775696644614146No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7610.12213951189134879No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7600.1219790131897833No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7480.12005302877099724No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7450.11957153266630074No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7270.11668255603812165No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7160.11491707032090111No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6820.10946011446767395No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6540.10496615082383984No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6510.10448465471914334No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6500.10432415601757783No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6320.10143517938939874No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG6280.10079318458313673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTCG250.00549443529.610
GGTATCA6350.027.9685041
CCGTGTC556.254013E-726.909099
GGTAGAC400.001930200523.1253
TAGGACA855.171205E-821.7647064
GTATCAA15600.020.9903851
CGAACTA3150.019.96825429
GCAGCGC759.254609E-619.73333417
GCGAACT3300.019.06060628
AAAGCGA3350.018.77611725
GTACATG3900.018.51
ATAGGAC801.6147354E-518.53
GACGCCG500.00703135318.513
GGACCGT801.6147354E-518.56
ATTACCC3550.018.2394373
CAAAGCG3500.017.97142824
ACCCTCA3550.017.7183116
CGGAGTT1156.391019E-817.69565224
TTGGTAG953.6010279E-617.5263164
ATTGACG953.6010279E-617.52631617
Produced by FastQC (version 0.11.5)