##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623058
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814046204366207	33.0	33.0	33.0	27.0	33.0
2	31.92289000381987	33.0	33.0	33.0	33.0	33.0
3	31.64548725800808	33.0	33.0	33.0	27.0	33.0
4	31.936481033868436	33.0	33.0	33.0	33.0	33.0
5	32.03007264171233	33.0	33.0	33.0	33.0	33.0
6	35.18350940040895	37.0	37.0	37.0	33.0	37.0
7	35.31931698172562	37.0	37.0	37.0	33.0	37.0
8	35.44461189808975	37.0	37.0	37.0	33.0	37.0
9	35.564782090912885	37.0	37.0	37.0	33.0	37.0
10	35.567627732891644	37.0	37.0	37.0	33.0	37.0
11	35.59234934789377	37.0	37.0	37.0	33.0	37.0
12	35.56362168530057	37.0	37.0	37.0	33.0	37.0
13	35.57442966786399	37.0	37.0	37.0	33.0	37.0
14	35.552972596451696	37.0	37.0	37.0	33.0	37.0
15	35.57700246205008	37.0	37.0	37.0	33.0	37.0
16	35.56676424987722	37.0	37.0	37.0	33.0	37.0
17	35.55520994835152	37.0	37.0	37.0	33.0	37.0
18	35.530621547271686	37.0	37.0	37.0	33.0	37.0
19	35.52959596056868	37.0	37.0	37.0	33.0	37.0
20	35.516309878053086	37.0	37.0	37.0	33.0	37.0
21	35.52653525032982	37.0	37.0	37.0	33.0	37.0
22	35.434874441865766	37.0	37.0	37.0	33.0	37.0
23	35.48710713930324	37.0	37.0	37.0	33.0	37.0
24	35.47177309335567	37.0	37.0	37.0	33.0	37.0
25	35.495465911680775	37.0	37.0	37.0	33.0	37.0
26	35.38713089311108	37.0	37.0	37.0	33.0	37.0
27	35.400893656770315	37.0	37.0	37.0	33.0	37.0
28	35.431325815574155	37.0	37.0	37.0	33.0	37.0
29	35.4241643635103	37.0	37.0	37.0	33.0	37.0
30	35.42351915873001	37.0	37.0	37.0	33.0	37.0
31	35.41998016236049	37.0	37.0	37.0	33.0	37.0
32	35.403959181970215	37.0	37.0	37.0	33.0	37.0
33	35.41307069325809	37.0	37.0	37.0	33.0	37.0
34	35.394340494785396	37.0	37.0	37.0	33.0	37.0
35	35.32634521986717	37.0	37.0	37.0	33.0	37.0
36	35.34999630852987	37.0	37.0	37.0	33.0	37.0
37	35.34521344722321	37.0	37.0	37.0	33.0	37.0
38	35.33796853583455	37.0	37.0	37.0	33.0	37.0
39	35.24892546119302	37.0	37.0	37.0	33.0	37.0
40	35.04364280692969	37.0	37.0	37.0	27.0	37.0
41	35.21559790581294	37.0	37.0	37.0	33.0	37.0
42	35.25964677445759	37.0	37.0	37.0	33.0	37.0
43	34.93380712550035	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	34.0
17	34.0
18	19.0
19	25.0
20	37.0
21	129.0
22	341.0
23	807.0
24	1557.0
25	2670.0
26	4317.0
27	6581.0
28	9012.0
29	12362.0
30	16076.0
31	19783.0
32	25467.0
33	33501.0
34	48116.0
35	97274.0
36	344897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57260158765316	20.03248493719686	13.250612302546472	24.144301172603512
2	16.8507586773623	20.892918476289527	39.14755929624529	23.10876355010288
3	18.072635292380486	26.920768210985173	29.240455944711407	25.766140551922934
4	12.432710919368663	17.450221327709457	39.23262360807501	30.884444144846867
5	14.196431150871987	36.892392040548394	34.80863739812345	14.102539410456169
6	30.6042134119135	39.11674354554472	16.729903155083477	13.549139887458312
7	28.239425543047357	31.43046072757271	22.672207081844707	17.65790664753522
8	24.97841292463944	35.718344038596726	19.870060251212568	19.433182785551264
9	26.315206609978524	15.430666165910717	19.93698820976538	38.31713901434537
10	16.10989667093593	27.504341489877348	32.84814575850081	23.537616080685908
11	34.37561190129972	23.80885888633161	23.018242282419934	18.797286929948736
12	24.25937874162598	25.674495793329033	28.863283996032475	21.202841469012515
13	27.72390371361896	22.037113719750007	25.855859326098052	24.383123240532985
14	22.47431218281444	20.630182101826797	27.221863775122056	29.673641940236706
15	24.50253427449772	27.960799797129642	25.031056498752925	22.505609429619714
16	23.829723717535124	27.93239152695255	24.386012217161163	23.851872538351166
17	23.15867864628975	27.12540405548119	25.261693132902558	24.4542241653265
18	23.101380609830866	26.69093407034337	27.900131287937878	22.307554031887882
19	26.231105290358204	25.245322265342875	26.63989548324554	21.883676961053386
20	24.804753329545566	25.90095946123796	27.32570643503494	21.968580774181536
21	23.68110191988547	26.607153748126176	27.083192896969464	22.62855143501889
22	23.606470023657508	26.34971383081511	26.81724654847542	23.22656959705196
23	23.19864282297956	26.503311088213295	27.596467744575946	22.701578344231198
24	23.799870959043943	27.264235432335354	25.519775045019887	23.416118563600822
25	23.40648864150689	26.84693880826504	27.42345014428833	22.323122405939735
26	24.19036429995281	26.354689290563638	27.165207733469437	22.28973867601411
27	23.894725691669155	26.38711002827987	25.796956302623514	23.921207977427464
28	22.735443570261516	26.096446879744743	27.979257147809673	23.188852402184065
29	24.91164546478819	26.263686526776	26.679057166427523	22.14561084200829
30	24.05522439323466	25.755066141514916	27.292001707706188	22.897707757544243
31	23.252409888004006	25.89662599629569	27.460846341753097	23.390117773947207
32	22.181081055054264	25.944615108063772	27.58314635234601	24.29115748453595
33	22.716344224775224	26.0789525212741	27.859204119038676	23.345499134912
34	23.80163644476116	25.995332697758478	27.71603927724225	22.486991580238115
35	23.942393806034108	26.77760336918874	26.37683811137968	22.90316471339747
36	24.336097120974294	26.327083513894372	26.07734753425845	23.259471830872886
37	23.46137919744229	25.81156168446597	27.032475307274762	23.694583810816972
38	23.879638813722	26.016358027663557	26.65241438196765	23.451588776646798
39	22.98437705638961	26.30397170086894	27.264877427141617	23.44677381559983
40	23.600852569102713	26.85624773295584	27.07661245020528	22.466287247736165
41	23.07955278641796	25.642396053015933	27.468389780726675	23.809661379839437
42	22.434829502229327	27.55136760943604	26.794134735449994	23.219668152884644
43	24.255045276683713	24.881792706297006	26.876470569353096	23.98669144766619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	39.5
2	38.0
3	183.5
4	329.0
5	329.0
6	377.5
7	426.0
8	384.5
9	343.0
10	453.0
11	563.0
12	563.0
13	922.0
14	1281.0
15	2346.5
16	3412.0
17	3980.0
18	4548.0
19	4548.0
20	4133.0
21	3718.0
22	3959.5
23	4201.0
24	5389.5
25	6578.0
26	6578.0
27	8592.0
28	10606.0
29	12513.0
30	14420.0
31	17512.0
32	20604.0
33	20604.0
34	26731.0
35	32858.0
36	36054.5
37	39251.0
38	41664.0
39	44077.0
40	44077.0
41	45906.5
42	47736.0
43	49135.5
44	50535.0
45	48415.5
46	46296.0
47	46296.0
48	43588.0
49	40880.0
50	42094.5
51	43309.0
52	42804.5
53	42300.0
54	42300.0
55	43373.0
56	44446.0
57	37409.5
58	30373.0
59	27825.5
60	25278.0
61	25278.0
62	23473.5
63	21669.0
64	16903.5
65	12138.0
66	10048.0
67	7958.0
68	7958.0
69	6902.5
70	5847.0
71	5764.0
72	5681.0
73	5757.5
74	5834.0
75	5834.0
76	4488.0
77	3142.0
78	2275.5
79	1409.0
80	886.5
81	364.0
82	364.0
83	298.0
84	232.0
85	210.5
86	189.0
87	142.5
88	96.0
89	96.0
90	65.0
91	34.0
92	24.0
93	14.0
94	9.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	623058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.41577896720341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09058068480516	40.820519449089126
2	7.406631798087358	7.023824324591412
3	1.9600152619947904	2.7880695130527076
4	0.9249114035238546	1.754215786949319
5	0.5801333702545234	1.3753737827743628
6	0.4065564697902003	1.156631502555519
7	0.3054677124097259	1.0138792673265773
8	0.2358131710079057	0.8945012155252957
9	0.1848821810612355	0.7889699368956621
>10	1.4928908390531632	14.5212106895853
>50	0.22933713563755687	7.693209577424573
>100	0.16864038960598796	14.690306878294024
>500	0.011380639789361153	3.470913474388826
>1k	0.002758942979239067	2.008374601547327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	0.4564583072522943	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1853	0.2974040940008795	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1426	0.2288711484324092	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1336	0.2144262652915138	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1218	0.19548741850678428	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1211	0.19436392759582577	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1210	0.19420342889426026	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1184	0.19003046265355714	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	984	0.15793072234045627	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	852	0.13674489373380969	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	796	0.12775696644614146	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	761	0.12213951189134879	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	760	0.1219790131897833	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	748	0.12005302877099724	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	745	0.11957153266630074	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	727	0.11668255603812165	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	716	0.11491707032090111	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	682	0.10946011446767395	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	654	0.10496615082383984	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	651	0.10448465471914334	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	650	0.10432415601757783	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	632	0.10143517938939874	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	628	0.10079318458313673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.6049870156550433E-4	0.0	0.0
5	0.0	0.0	3.2099740313100867E-4	0.0	0.0
6	0.0	0.0	6.419948062620173E-4	0.0	0.0
7	0.0	0.0	0.0016049870156550434	3.2099740313100867E-4	0.0
8	1.6049870156550433E-4	0.0	0.0017654857172205477	4.81496104696513E-4	0.0
9	3.2099740313100867E-4	0.0	0.0020864831203515565	0.0020864831203515565	0.0
10	3.2099740313100867E-4	0.0	0.0020864831203515565	0.0020864831203515565	0.0
11	3.2099740313100867E-4	0.0	0.002888976628179078	0.0020864831203515565	0.0
12	3.2099740313100867E-4	0.0	0.003049475329744582	0.002407480523482565	0.0
13	3.2099740313100867E-4	0.0	0.003209974031310087	0.002407480523482565	0.0
14	3.2099740313100867E-4	0.0	0.003209974031310087	0.002407480523482565	0.0
15	3.2099740313100867E-4	0.0	0.003370472732875591	0.002407480523482565	0.0
16	3.2099740313100867E-4	0.0	0.003370472732875591	0.0025679792250480693	0.0
17	3.2099740313100867E-4	0.0	0.003370472732875591	0.0027284779266135736	0.0
18	3.2099740313100867E-4	0.0	0.0036914701360065997	0.0027284779266135736	0.0
19	3.2099740313100867E-4	0.0	0.003851968837572104	0.0027284779266135736	0.0
20	3.2099740313100867E-4	0.0	0.004012467539137608	0.002888976628179078	0.0
21	3.2099740313100867E-4	0.0	0.004172966240703113	0.003209974031310087	0.0
22	3.2099740313100867E-4	0.0	0.004333464942268617	0.003370472732875591	0.0
23	6.419948062620173E-4	0.0	0.004333464942268617	0.004012467539137608	0.0
24	6.419948062620173E-4	0.0	0.004333464942268617	0.004654462345399625	0.0
25	6.419948062620173E-4	0.0	0.004333464942268617	0.005296457151661643	0.0
26	6.419948062620173E-4	0.0	0.004333464942268617	0.005456955853227147	0.0
27	6.419948062620173E-4	0.0	0.004333464942268617	0.007061942868882191	0.0
28	6.419948062620173E-4	0.0	0.004333464942268617	0.014123885737764382	0.0
29	6.419948062620173E-4	0.0	0.004333464942268617	0.02776627537083225	0.0
30	6.419948062620173E-4	0.0	0.004333464942268617	0.04654462345399626	0.0
31	6.419948062620173E-4	0.0	0.004333464942268617	0.09100276378764095	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTTCG	25	0.005494435	29.6	10
GGTATCA	635	0.0	27.968504	1
CCGTGTC	55	6.254013E-7	26.90909	9
GGTAGAC	40	0.0019302005	23.125	3
TAGGACA	85	5.171205E-8	21.764706	4
GTATCAA	1560	0.0	20.990385	1
CGAACTA	315	0.0	19.968254	29
GCAGCGC	75	9.254609E-6	19.733334	17
GCGAACT	330	0.0	19.060606	28
AAAGCGA	335	0.0	18.776117	25
GTACATG	390	0.0	18.5	1
ATAGGAC	80	1.6147354E-5	18.5	3
GACGCCG	50	0.007031353	18.5	13
GGACCGT	80	1.6147354E-5	18.5	6
ATTACCC	355	0.0	18.239437	3
CAAAGCG	350	0.0	17.971428	24
ACCCTCA	355	0.0	17.718311	6
CGGAGTT	115	6.391019E-8	17.695652	24
TTGGTAG	95	3.6010279E-6	17.526316	4
ATTGACG	95	3.6010279E-6	17.526316	17
>>END_MODULE