FastQCFastQC Report
Fri 10 Feb 2017
ERR1633157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772810
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28630.3704662206751983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.24896158176007038No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12850.1662763163002549No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12480.16148859357409973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11810.15281893350241327No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11550.14945458780295284No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11480.1485488024223289No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10320.13353864468627477No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9610.1243513929685175No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA8830.11425835587013625No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8210.10623568535603836No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8180.10584749162148523No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7990.10338893130264878No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG7910.1023537480105071No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7870.10183615636443627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.026.7222231
GTATCAA17200.023.2325571
CGAGTCG652.6809576E-622.7692321
CGCCGTC753.7374775E-722.226
CGTCCGC705.0983544E-621.14285729
TTAACGG2600.020.63461535
ACGTATA450.003824690420.55555729
TAACGGC2700.019.8703736
ATTAGAG2900.019.7758623
GTCAACC3000.019.73333415
TTTAACG2800.019.16071334
GCACCGC2950.018.81355910
ATTACCC3350.018.776123
TATTAGA3450.018.2318842
TACCCTC3500.017.971435
AACGGCC2900.017.86206837
CAACCCA3250.017.64615417
GTATTAG4300.017.6395341
CACCGCC3150.017.6190511
CGCGATA953.6032961E-617.52631434
Produced by FastQC (version 0.11.5)