##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772810
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758728536121428	33.0	33.0	33.0	27.0	33.0
2	31.89487843066213	33.0	33.0	33.0	33.0	33.0
3	31.617842678019176	33.0	33.0	33.0	27.0	33.0
4	31.905029696820694	33.0	33.0	33.0	33.0	33.0
5	32.01298378644169	33.0	33.0	33.0	33.0	33.0
6	35.14820977989415	37.0	37.0	37.0	33.0	37.0
7	35.294067105756916	37.0	37.0	37.0	33.0	37.0
8	35.428532239489655	37.0	37.0	37.0	33.0	37.0
9	35.55383858904517	37.0	37.0	37.0	33.0	37.0
10	35.55292762774809	37.0	37.0	37.0	33.0	37.0
11	35.5659308238765	37.0	37.0	37.0	33.0	37.0
12	35.54001501015774	37.0	37.0	37.0	33.0	37.0
13	35.551786338168505	37.0	37.0	37.0	33.0	37.0
14	35.53520399580751	37.0	37.0	37.0	33.0	37.0
15	35.55190797220533	37.0	37.0	37.0	33.0	37.0
16	35.539005706447895	37.0	37.0	37.0	33.0	37.0
17	35.519316520231364	37.0	37.0	37.0	33.0	37.0
18	35.5088585810225	37.0	37.0	37.0	33.0	37.0
19	35.50285451792808	37.0	37.0	37.0	33.0	37.0
20	35.50382759022269	37.0	37.0	37.0	33.0	37.0
21	35.50270441635072	37.0	37.0	37.0	33.0	37.0
22	35.40770046971442	37.0	37.0	37.0	33.0	37.0
23	35.46009627204617	37.0	37.0	37.0	33.0	37.0
24	35.44352686947633	37.0	37.0	37.0	33.0	37.0
25	35.465901062356856	37.0	37.0	37.0	33.0	37.0
26	35.36883580699008	37.0	37.0	37.0	33.0	37.0
27	35.36070314825119	37.0	37.0	37.0	33.0	37.0
28	35.39434401728756	37.0	37.0	37.0	33.0	37.0
29	35.39306168398442	37.0	37.0	37.0	33.0	37.0
30	35.39416933010701	37.0	37.0	37.0	33.0	37.0
31	35.38141457796871	37.0	37.0	37.0	33.0	37.0
32	35.381270946286925	37.0	37.0	37.0	33.0	37.0
33	35.391941098070674	37.0	37.0	37.0	33.0	37.0
34	35.37340614122488	37.0	37.0	37.0	33.0	37.0
35	35.29776788602632	37.0	37.0	37.0	33.0	37.0
36	35.3405144860962	37.0	37.0	37.0	33.0	37.0
37	35.3410696031366	37.0	37.0	37.0	33.0	37.0
38	35.33595191573608	37.0	37.0	37.0	33.0	37.0
39	35.23688875661547	37.0	37.0	37.0	33.0	37.0
40	35.02878844735446	37.0	37.0	37.0	27.0	37.0
41	35.206394844787205	37.0	37.0	37.0	33.0	37.0
42	35.24526727138624	37.0	37.0	37.0	33.0	37.0
43	34.915182256958374	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	26.0
16	30.0
17	20.0
18	24.0
19	31.0
20	44.0
21	136.0
22	401.0
23	977.0
24	1942.0
25	3442.0
26	5686.0
27	8347.0
28	11615.0
29	15522.0
30	19928.0
31	25302.0
32	32140.0
33	41937.0
34	60032.0
35	120919.0
36	424299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24427737736313	20.24443265485695	13.404329654119381	24.10696031366054
2	16.545463956211748	21.303813356452427	39.05526584800921	23.095456839326616
3	17.980616192854647	26.967301147759475	29.492760186850585	25.559322472535296
4	12.349995471073097	17.773061942780245	39.51255806731279	30.364384518833866
5	14.046790284804803	36.713293047450215	35.3196775404045	13.920239127340484
6	30.111023408082193	39.627981004386584	16.967430545670993	13.293565041860225
7	27.83323197163598	31.569079075063726	22.956354084445078	17.641334868855214
8	24.699214554677088	35.827952536846055	20.11115280599371	19.361680102483145
9	26.11625108370751	15.39408133952718	20.266689095637997	38.22297848112731
10	15.61030524967327	27.762839507770344	33.56219510617099	23.0646601363854
11	34.24865102677243	23.79860509051384	23.449360127327544	18.50338375538619
12	23.62016537053092	25.678368551131587	29.642214774653535	21.05925130368396
13	27.895860560810547	22.30134185634244	26.000051759164606	23.802745823682407
14	22.414435631008917	20.86036671368124	27.284326030977862	29.440871624331983
15	24.123005654688733	28.54453229124882	24.817872439538828	22.514589614523622
16	23.66066691683596	28.307734113171414	24.717330262289565	23.314268707703057
17	23.11421953649668	27.136683013936153	25.642784125464214	24.10631332410295
18	23.134535008604963	26.99033397600963	27.69826994992301	22.176861065462404
19	25.764288764379344	25.833646044952836	26.9623840271218	21.43968116354602
20	24.89848733841436	26.157270221658624	27.261551998550743	21.682690441376277
21	23.30844580168476	26.960831252183592	27.102651363207	22.62807158292465
22	23.40963496849161	26.435993323067763	27.08350047230238	23.07087123613825
23	22.846754053389578	26.65428759979814	27.885379329977617	22.613579016834667
24	23.385437559037797	27.467165279952383	25.926812541245585	23.220584619764235
25	23.187458754415704	27.19555906367671	27.354071505285905	22.26291067662168
26	23.932273133111632	26.704235193643978	26.915412585240873	22.44807908800352
27	23.475886699188674	26.78019176770487	26.072255793791488	23.671665739314967
28	22.924522198211722	26.44479238105097	27.68468316921365	22.946002251523662
29	24.50265912708169	26.67913199880954	26.714716424476908	22.10349244963186
30	23.86589200450305	26.205535642654727	26.958760885599307	22.969811467242916
31	23.33510177145741	26.205794438477763	27.235025426689614	23.224078363375213
32	22.26226368706409	26.493057802047076	27.441415095560355	23.803263415328477
33	22.61681396462261	26.277222085635536	28.06407784578357	23.04188610395828
34	23.670501158111307	26.121427000168218	27.81615144731564	22.391920394404835
35	23.580957803341054	27.04998641321929	26.38837489162925	22.980680891810408
36	23.955047165538748	26.545334558300226	26.302842872116045	23.196775404044978
37	23.257333626635265	26.054916473648117	27.14638785729998	23.541362042416637
38	23.38414357992262	26.20657082594687	26.79597831291003	23.61330728122048
39	22.86771651505545	26.464072669867107	27.365717317322495	23.30249349775495
40	23.426715492811944	27.22350901256454	27.057103298352764	22.29267219627075
41	23.167531476041976	25.84283329667059	27.447755593224727	23.541879634062706
42	22.396708117130988	27.642240654235838	26.869088132917536	23.091963095715634
43	24.013276225721718	25.43652385450499	26.868570541271463	23.68162937850183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	35.0
2	46.0
3	176.0
4	306.0
5	306.0
6	368.0
7	430.0
8	394.5
9	359.0
10	486.5
11	614.0
12	614.0
13	1006.5
14	1399.0
15	2478.0
16	3557.0
17	4216.5
18	4876.0
19	4876.0
20	4684.5
21	4493.0
22	5149.0
23	5805.0
24	7499.5
25	9194.0
26	9194.0
27	11721.5
28	14249.0
29	17155.5
30	20062.0
31	23786.0
32	27510.0
33	27510.0
34	35009.0
35	42508.0
36	46449.0
37	50390.0
38	53174.0
39	55958.0
40	55958.0
41	57500.5
42	59043.0
43	60628.5
44	62214.0
45	60028.0
46	57842.0
47	57842.0
48	55211.5
49	52581.0
50	53447.0
51	54313.0
52	53263.0
53	52213.0
54	52213.0
55	51759.0
56	51305.0
57	43539.5
58	35774.0
59	33453.0
60	31132.0
61	31132.0
62	28493.0
63	25854.0
64	20484.0
65	15114.0
66	12728.5
67	10343.0
68	10343.0
69	8843.5
70	7344.0
71	6915.5
72	6487.0
73	5749.5
74	5012.0
75	5012.0
76	3451.0
77	1890.0
78	1484.5
79	1079.0
80	854.0
81	629.0
82	629.0
83	490.5
84	352.0
85	299.0
86	246.0
87	200.0
88	154.0
89	154.0
90	116.0
91	78.0
92	51.0
93	24.0
94	15.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	772810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.09029105135298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94511112612109	47.02886366304118
2	7.477289982234213	8.08897582828829
3	1.8273367537107281	2.965235305711434
4	0.8701258631589671	1.8826144475831303
5	0.5206115798171637	1.408001593850753
6	0.3306139700660294	1.0729803519908896
7	0.2571205539438425	0.9735407918675282
8	0.18813111572728197	0.8140853444403561
9	0.15281087803786358	0.7439026381992759
>10	1.1455823171719015	12.511166305975689
>50	0.1545191213683326	5.87656610824187
>100	0.11983096268920546	11.92158913381367
>500	0.00897519356168334	3.159496657948066
>1k	0.001940582391715317	1.552981829047895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2863	0.3704662206751983	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1924	0.24896158176007038	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1285	0.1662763163002549	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1248	0.16148859357409973	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1181	0.15281893350241327	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1155	0.14945458780295284	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1148	0.1485488024223289	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1032	0.13353864468627477	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	961	0.1243513929685175	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	883	0.11425835587013625	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	821	0.10623568535603836	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	818	0.10584749162148523	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	799	0.10338893130264878	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	791	0.1023537480105071	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	787	0.10183615636443627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.293979115177081E-4	0.0	0.0
4	0.0	0.0	2.587958230354162E-4	0.0	0.0
5	0.0	0.0	3.8819373455312433E-4	0.0	0.0
6	0.0	0.0	5.175916460708324E-4	0.0	0.0
7	1.293979115177081E-4	0.0	0.001164581203659373	0.0	0.0
8	1.293979115177081E-4	0.0	0.0014233770266947891	0.0	0.0
9	1.293979115177081E-4	0.0	0.0018115707612479134	3.8819373455312433E-4	0.0
10	2.587958230354162E-4	0.0	0.0019409686727656216	5.175916460708324E-4	0.0
11	2.587958230354162E-4	0.0	0.0028467540533895783	5.175916460708324E-4	0.0
12	2.587958230354162E-4	0.0	0.0029761519649072862	5.175916460708324E-4	0.0
13	2.587958230354162E-4	0.0	0.0031055498764249946	6.469895575885405E-4	0.0
14	2.587958230354162E-4	0.0	0.0032349477879427026	7.763874691062487E-4	0.0
15	2.587958230354162E-4	0.0	0.0033643456994604105	7.763874691062487E-4	0.0
16	3.8819373455312433E-4	0.0	0.004011335257048951	7.763874691062487E-4	0.0
17	3.8819373455312433E-4	0.0	0.0041407331685666595	7.763874691062487E-4	0.0
18	3.8819373455312433E-4	0.0	0.0041407331685666595	7.763874691062487E-4	0.0
19	3.8819373455312433E-4	0.0	0.004270131080084367	0.001293979115177081	0.0
20	3.8819373455312433E-4	0.0	0.004270131080084367	0.0015527749382124973	0.0
21	3.8819373455312433E-4	0.0	0.004270131080084367	0.0019409686727656216	0.0
22	3.8819373455312433E-4	0.0	0.004399528991602075	0.002458560318836454	0.0
23	3.8819373455312433E-4	0.0	0.004399528991602075	0.0027173561418718704	0.0
24	3.8819373455312433E-4	0.0	0.004399528991602075	0.0032349477879427026	0.0
25	3.8819373455312433E-4	0.0	0.004528926903119784	0.0033643456994604105	0.0
26	3.8819373455312433E-4	0.0	0.004528926903119784	0.003493743610978119	0.0
27	3.8819373455312433E-4	0.0	0.004528926903119784	0.004399528991602075	0.0
28	3.8819373455312433E-4	0.0	0.004528926903119784	0.010093037098381233	0.0
29	3.8819373455312433E-4	0.0	0.004658324814637492	0.024197409453811415	0.0
30	3.8819373455312433E-4	0.0	0.004658324814637492	0.04580686067726867	0.0
31	3.8819373455312433E-4	0.0	0.004658324814637492	0.10170675845291857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	630	0.0	26.722223	1
GTATCAA	1720	0.0	23.232557	1
CGAGTCG	65	2.6809576E-6	22.76923	21
CGCCGTC	75	3.7374775E-7	22.2	26
CGTCCGC	70	5.0983544E-6	21.142857	29
TTAACGG	260	0.0	20.634615	35
ACGTATA	45	0.0038246904	20.555557	29
TAACGGC	270	0.0	19.87037	36
ATTAGAG	290	0.0	19.775862	3
GTCAACC	300	0.0	19.733334	15
TTTAACG	280	0.0	19.160713	34
GCACCGC	295	0.0	18.813559	10
ATTACCC	335	0.0	18.77612	3
TATTAGA	345	0.0	18.231884	2
TACCCTC	350	0.0	17.97143	5
AACGGCC	290	0.0	17.862068	37
CAACCCA	325	0.0	17.646154	17
GTATTAG	430	0.0	17.639534	1
CACCGCC	315	0.0	17.61905	11
CGCGATA	95	3.6032961E-6	17.526314	34
>>END_MODULE