FastQCFastQC Report
Fri 10 Feb 2017
ERR1633156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747182
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28110.376213559748495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.24920300542571958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11760.1573913718478229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.028.3281271
ACCGGAA400.001930557723.12500225
GTATCAA15750.021.9650781
CACGGTA851.2441142E-619.5882362
TTAACGG1451.546141E-1017.86206835
TAACGGC1502.5102054E-1017.26666636
GTATACC650.001579234417.0769231
AACGGCC1554.0017767E-1016.70967737
CCGTACA1452.9740477E-916.58620617
TATAGTG904.442606E-516.4444455
GGACGCC700.002591355715.85714213
GATCTTA700.002591355715.85714211
AGCGTTC1302.5852205E-715.65384637
CGCGATA957.05439E-515.57894834
ACGGTAC957.05439E-515.5789483
CCGCTGA855.3610286E-415.23529526
CCCCGTA1701.4824764E-915.23529515
TCAACCC1101.4504369E-515.13636416
GTGTAGA1353.9664337E-715.0740751
GTATTAG3200.015.0312511
Produced by FastQC (version 0.11.5)