##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747182
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813274409715437	33.0	33.0	33.0	27.0	33.0
2	31.951179230763053	33.0	33.0	33.0	33.0	33.0
3	31.670437724677523	33.0	33.0	33.0	27.0	33.0
4	31.94484208666697	33.0	33.0	33.0	33.0	33.0
5	32.04546951077515	33.0	33.0	33.0	33.0	33.0
6	35.20625363030694	37.0	37.0	37.0	33.0	37.0
7	35.34094638254133	37.0	37.0	37.0	33.0	37.0
8	35.46978647772564	37.0	37.0	37.0	33.0	37.0
9	35.592890353354335	37.0	37.0	37.0	33.0	37.0
10	35.59369470891965	37.0	37.0	37.0	33.0	37.0
11	35.61778254829479	37.0	37.0	37.0	33.0	37.0
12	35.60700605742644	37.0	37.0	37.0	33.0	37.0
13	35.60103294779585	37.0	37.0	37.0	33.0	37.0
14	35.58650235150204	37.0	37.0	37.0	33.0	37.0
15	35.59238445251626	37.0	37.0	37.0	33.0	37.0
16	35.591720624961525	37.0	37.0	37.0	33.0	37.0
17	35.58309220511201	37.0	37.0	37.0	33.0	37.0
18	35.56353739784952	37.0	37.0	37.0	33.0	37.0
19	35.551449312215766	37.0	37.0	37.0	33.0	37.0
20	35.56316399484998	37.0	37.0	37.0	33.0	37.0
21	35.56026242602204	37.0	37.0	37.0	33.0	37.0
22	35.45531075427406	37.0	37.0	37.0	33.0	37.0
23	35.506655674253395	37.0	37.0	37.0	33.0	37.0
24	35.50390400197007	37.0	37.0	37.0	33.0	37.0
25	35.51915597538485	37.0	37.0	37.0	33.0	37.0
26	35.40621294410197	37.0	37.0	37.0	33.0	37.0
27	35.42094697142062	37.0	37.0	37.0	33.0	37.0
28	35.4310971088704	37.0	37.0	37.0	33.0	37.0
29	35.44374195309844	37.0	37.0	37.0	33.0	37.0
30	35.44218008463801	37.0	37.0	37.0	33.0	37.0
31	35.45514078229936	37.0	37.0	37.0	33.0	37.0
32	35.443407362597064	37.0	37.0	37.0	33.0	37.0
33	35.43081605284924	37.0	37.0	37.0	33.0	37.0
34	35.41572869796114	37.0	37.0	37.0	33.0	37.0
35	35.35541139909687	37.0	37.0	37.0	33.0	37.0
36	35.387755593684005	37.0	37.0	37.0	33.0	37.0
37	35.388151748837636	37.0	37.0	37.0	33.0	37.0
38	35.37330529911053	37.0	37.0	37.0	33.0	37.0
39	35.278668383338996	37.0	37.0	37.0	33.0	37.0
40	35.077932819580774	37.0	37.0	37.0	33.0	37.0
41	35.24556667585675	37.0	37.0	37.0	33.0	37.0
42	35.29923766900166	37.0	37.0	37.0	33.0	37.0
43	34.9697757708296	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	14.0
16	29.0
17	35.0
18	23.0
19	26.0
20	47.0
21	112.0
22	348.0
23	893.0
24	1689.0
25	3124.0
26	5189.0
27	7776.0
28	10698.0
29	14321.0
30	18567.0
31	23803.0
32	30237.0
33	40005.0
34	57167.0
35	115372.0
36	417701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53512798755858	20.057362195556102	13.754212494412338	24.653297322472973
2	16.097149021255866	21.171548565142093	38.98635138426782	23.744951029334217
3	18.215508403575033	26.520713828759256	29.561739977676122	25.70203778998959
4	12.618210824136556	17.56733968430717	38.894405914489376	30.920043577066895
5	14.290761822420775	36.469160124307066	34.9669826093241	14.273095443948062
6	30.45991471957301	38.95249082552845	17.190858452157574	13.396736002740967
7	27.1888241419092	31.953259045319616	22.875417234355215	17.98249957841597
8	24.85592533010699	35.84708946414662	20.283813046888174	19.013172158858215
9	26.070221177705033	15.635146456954264	20.006905947948425	38.28772641739228
10	15.086685707096798	28.177472155378474	33.51271845413835	23.223123683386376
11	34.476472934305164	23.396709235500857	23.751776675562315	18.37504115463167
12	23.166377134352807	25.374808279642714	30.295563865296543	21.16325072070794
13	28.31853551075909	22.01297675800541	25.82342722388923	23.84506050734627
14	22.06196080740703	20.808584789248137	27.570792658281384	29.558661745063453
15	23.766632493823458	28.685782045070678	24.157166527030896	23.390418934074965
16	23.063992440931393	28.358686370924353	24.929133731808314	23.64818745633594
17	22.75416163665613	27.61375407865821	26.228683239157256	23.403401045528398
18	23.43123897524298	26.948721998120938	27.449670896782845	22.170368129853234
19	24.944658731072213	26.332673966985286	26.882874587449912	21.839792714492585
20	24.492292373210276	26.212087550288953	27.152554531559915	22.143065544940857
21	23.465902551185653	26.563139904333884	27.255206897382433	22.71575064709803
22	23.0803204573986	26.558321801114054	27.29080732672896	23.070550414758387
23	23.093436405052582	26.38098883538415	27.50093016159383	23.024644597969438
24	23.100931232283433	26.750912093706752	26.90870497415623	23.239451699853582
25	23.39764608890471	26.482570511602262	27.381815943103554	22.737967456389473
26	23.501502980532184	26.554039042696427	27.0809521642652	22.86350581250619
27	23.213085968345062	26.722137310588316	26.99984742673137	23.064929294335247
28	22.978203436378287	26.662981709944834	27.212513149406703	23.14630170427018
29	23.744415684532015	26.53289292300939	26.85677652834249	22.865914864116103
30	23.30837734313728	26.420470514546658	27.355851720196682	22.91530042211938
31	23.244135966872864	26.451119004472805	27.15148384195551	23.153261186698824
32	22.642809917797805	26.441348961832595	27.416479519046227	23.499361601323372
33	22.686039010575737	26.471060598354885	27.80955644006414	23.033343951005243
34	23.130241360204074	26.294262977427184	27.340059048531685	23.235436613837056
35	23.036422183617912	26.680514252216998	27.191500865920215	23.09156269824487
36	23.71724693582019	26.431846591593484	26.921553249409115	22.929353223177216
37	23.47942000744129	26.147980010225087	27.080282983262443	23.29231699907118
38	23.198363986284466	26.49782783846506	27.081755181468502	23.22205299378197
39	22.88478576839378	26.341373320021095	27.341798919138842	23.432041992446283
40	22.961607747509976	26.78985842806706	27.421832967068266	22.8267008573547
41	23.02517994277164	26.132722683362285	27.458236413618103	23.383860960247972
42	22.97740041917498	26.596465118271052	27.448600207178437	22.97753425537553
43	23.247214199485533	26.118536046103895	27.266181465827604	23.36806828858297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	36.0
2	49.0
3	178.5
4	308.0
5	308.0
6	379.0
7	450.0
8	412.0
9	374.0
10	489.5
11	605.0
12	605.0
13	1019.5
14	1434.0
15	2553.0
16	3672.0
17	4447.0
18	5222.0
19	5222.0
20	5132.0
21	5042.0
22	5735.5
23	6429.0
24	8456.0
25	10483.0
26	10483.0
27	13259.5
28	16036.0
29	19259.0
30	22482.0
31	25940.5
32	29399.0
33	29399.0
34	34026.0
35	38653.0
36	41476.0
37	44299.0
38	46862.5
39	49426.0
40	49426.0
41	51618.0
42	53810.0
43	54746.0
44	55682.0
45	55555.0
46	55428.0
47	55428.0
48	54789.5
49	54151.0
50	53963.0
51	53775.0
52	52061.0
53	50347.0
54	50347.0
55	48049.0
56	45751.0
57	41405.0
58	37059.0
59	34368.0
60	31677.0
61	31677.0
62	28307.0
63	24937.0
64	20998.5
65	17060.0
66	14600.5
67	12141.0
68	12141.0
69	10136.0
70	8131.0
71	6726.0
72	5321.0
73	4221.5
74	3122.0
75	3122.0
76	2420.0
77	1718.0
78	1399.0
79	1080.0
80	887.5
81	695.0
82	695.0
83	550.5
84	406.0
85	344.5
86	283.0
87	211.5
88	140.0
89	140.0
90	100.0
91	60.0
92	38.0
93	16.0
94	11.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	747182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.75000369810184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.40313047857148	57.67409278972221
2	8.260772829339464	11.028132338151767
3	2.1077789342029845	4.220827549584911
4	0.9496716160393617	2.5356233553043896
5	0.5157792900447863	1.721413475894691
6	0.34272572838490695	1.3726166182276311
7	0.23253071359842642	1.0864998194832054
8	0.17153377289814206	0.9159903980240273
9	0.12684054905640935	0.7619946406726131
>10	0.7768418059770023	9.779167489458903
>50	0.07452494228009918	3.4537779895751908
>100	0.03645177074562664	4.372651379646815
>500	8.100393493504109E-4	0.2865716866931641
>1k	6.075295120128082E-4	0.7906404695605267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.376213559748495	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1862	0.24920300542571958	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1176	0.1573913718478229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.338362005508698E-4	0.0	0.0
2	0.0	0.0	1.338362005508698E-4	0.0	0.0
3	0.0	0.0	1.338362005508698E-4	0.0	0.0
4	0.0	0.0	1.338362005508698E-4	2.676724011017396E-4	0.0
5	0.0	0.0	1.338362005508698E-4	2.676724011017396E-4	0.0
6	1.338362005508698E-4	0.0	4.015086016526094E-4	2.676724011017396E-4	0.0
7	1.338362005508698E-4	0.0	9.368534038560886E-4	4.015086016526094E-4	0.0
8	1.338362005508698E-4	0.0	0.0010706896044069584	4.015086016526094E-4	0.0
9	1.338362005508698E-4	0.0	0.0012045258049578282	0.0010706896044069584	0.0
10	1.338362005508698E-4	0.0	0.002141379208813917	0.0010706896044069584	0.0
11	1.338362005508698E-4	0.0	0.0036135774148734847	0.0012045258049578282	0.0
12	1.338362005508698E-4	0.0	0.0038812498159752243	0.0012045258049578282	0.0
13	1.338362005508698E-4	0.0	0.004282758417627834	0.0012045258049578282	0.0
14	1.338362005508698E-4	0.0	0.005085775620933052	0.001338362005508698	0.0
15	1.338362005508698E-4	0.0	0.005219611821483922	0.001338362005508698	0.0
16	1.338362005508698E-4	0.0	0.005353448022034792	0.001338362005508698	0.0
17	1.338362005508698E-4	0.0	0.005353448022034792	0.0016060344066104377	0.0
18	1.338362005508698E-4	0.0	0.006022629024789141	0.0016060344066104377	0.0
19	1.338362005508698E-4	0.0	0.006156465225340011	0.0017398706071613075	0.0
20	1.338362005508698E-4	0.0	0.006290301425890881	0.002141379208813917	0.0
21	1.338362005508698E-4	0.0	0.00655797382699262	0.002542887810466526	0.0
22	1.338362005508698E-4	0.0	0.00655797382699262	0.0028105602115682657	0.0
23	1.338362005508698E-4	0.0	0.00669181002754349	0.0030782326126700056	0.0
24	1.338362005508698E-4	0.0	0.00669181002754349	0.0032120688132208754	0.0
25	1.338362005508698E-4	0.0	0.00669181002754349	0.003479741214322615	0.0
26	1.338362005508698E-4	0.0	0.00669181002754349	0.0037474136154243545	0.0
27	1.338362005508698E-4	0.0	0.00669181002754349	0.004550430818729573	0.0
28	1.338362005508698E-4	0.0	0.00669181002754349	0.011911421849027413	0.0
29	1.338362005508698E-4	0.0	0.00669181002754349	0.024893533302461784	0.0
30	1.338362005508698E-4	0.0	0.00669181002754349	0.0398831877641592	0.0
31	1.338362005508698E-4	0.0	0.00669181002754349	0.08672585795696364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	640	0.0	28.328127	1
ACCGGAA	40	0.0019305577	23.125002	25
GTATCAA	1575	0.0	21.965078	1
CACGGTA	85	1.2441142E-6	19.588236	2
TTAACGG	145	1.546141E-10	17.862068	35
TAACGGC	150	2.5102054E-10	17.266666	36
GTATACC	65	0.0015792344	17.076923	1
AACGGCC	155	4.0017767E-10	16.709677	37
CCGTACA	145	2.9740477E-9	16.586206	17
TATAGTG	90	4.442606E-5	16.444445	5
GGACGCC	70	0.0025913557	15.857142	13
GATCTTA	70	0.0025913557	15.857142	11
AGCGTTC	130	2.5852205E-7	15.653846	37
CGCGATA	95	7.05439E-5	15.578948	34
ACGGTAC	95	7.05439E-5	15.578948	3
CCGCTGA	85	5.3610286E-4	15.235295	26
CCCCGTA	170	1.4824764E-9	15.235295	15
TCAACCC	110	1.4504369E-5	15.136364	16
GTGTAGA	135	3.9664337E-7	15.074075	1
GTATTAG	320	0.0	15.031251	1
>>END_MODULE