FastQCFastQC Report
Fri 10 Feb 2017
ERR1633155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777446
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41830.5380437998266118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.3835636172801712No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19190.2468338637024308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16650.21416278429627267No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14080.18110582599949063No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13200.1697867118745225No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11680.15023551474957747No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11620.14946375696832961No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10980.1412316739683528No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10890.14007403729648105No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9620.12373849759340198No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9260.11910795090591501No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8920.11473465681217732No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC8570.11023273642156498No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8430.10843196826532003No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8260.10624532121845118No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8030.1032869163903345No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7990.10277241120283595No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7890.10148614823408957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.00549526429.59999815
AACGGTT358.8668027E-426.428576
GGTATCA8050.025.049691
GTATCAA22500.023.268891
TTAGAGT400.001930628223.1254
AAACGGA400.001930628223.12532
CGTCCTA400.001930628223.12533
GCTTTAT4200.020.7023811
AACGGAG450.003824710420.55555733
TATCGGA450.003824710420.55555726
CGCTCTC1450.020.41379429
AACCACG656.898459E-519.92307735
TACTAGG656.898459E-519.9230772
TGCGGTA759.259444E-619.73333436
GCCGCTC1500.019.73333427
TTGCGGT759.259444E-619.73333435
GCTCGGA3600.019.52777911
CCGGCAG1551.8189894E-1219.09677316
GCCGGCA1650.019.06060615
TGGGGCG1750.019.02857237
Produced by FastQC (version 0.11.5)