##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633155.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	777446
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.827464544161266	33.0	33.0	33.0	27.0	33.0
2	31.94181589460876	33.0	33.0	33.0	33.0	33.0
3	31.663905917581413	33.0	33.0	33.0	27.0	33.0
4	31.95321218451185	33.0	33.0	33.0	33.0	33.0
5	32.05677950622937	33.0	33.0	33.0	33.0	33.0
6	35.215020721696426	37.0	37.0	37.0	33.0	37.0
7	35.36061668591773	37.0	37.0	37.0	33.0	37.0
8	35.475572579960534	37.0	37.0	37.0	33.0	37.0
9	35.5981109942041	37.0	37.0	37.0	33.0	37.0
10	35.60371009690705	37.0	37.0	37.0	33.0	37.0
11	35.622934583237935	37.0	37.0	37.0	33.0	37.0
12	35.60996905251297	37.0	37.0	37.0	33.0	37.0
13	35.610326633618286	37.0	37.0	37.0	33.0	37.0
14	35.592658525479585	37.0	37.0	37.0	33.0	37.0
15	35.605543021637516	37.0	37.0	37.0	33.0	37.0
16	35.59950273073628	37.0	37.0	37.0	33.0	37.0
17	35.587567496649285	37.0	37.0	37.0	33.0	37.0
18	35.569507078305115	37.0	37.0	37.0	33.0	37.0
19	35.570188797678554	37.0	37.0	37.0	33.0	37.0
20	35.564991009021846	37.0	37.0	37.0	33.0	37.0
21	35.561037036655925	37.0	37.0	37.0	33.0	37.0
22	35.46737265353478	37.0	37.0	37.0	33.0	37.0
23	35.518084857340575	37.0	37.0	37.0	33.0	37.0
24	35.51044445530622	37.0	37.0	37.0	33.0	37.0
25	35.528826439392574	37.0	37.0	37.0	33.0	37.0
26	35.42001373728851	37.0	37.0	37.0	33.0	37.0
27	35.43950448005392	37.0	37.0	37.0	33.0	37.0
28	35.4543749662356	37.0	37.0	37.0	33.0	37.0
29	35.45797264375918	37.0	37.0	37.0	33.0	37.0
30	35.45361221229513	37.0	37.0	37.0	33.0	37.0
31	35.4372458022808	37.0	37.0	37.0	33.0	37.0
32	35.43341659742284	37.0	37.0	37.0	33.0	37.0
33	35.42857767613442	37.0	37.0	37.0	33.0	37.0
34	35.40691572147776	37.0	37.0	37.0	33.0	37.0
35	35.34862099747121	37.0	37.0	37.0	33.0	37.0
36	35.37264453093848	37.0	37.0	37.0	33.0	37.0
37	35.36903270452224	37.0	37.0	37.0	33.0	37.0
38	35.34405990898403	37.0	37.0	37.0	33.0	37.0
39	35.254053657746006	37.0	37.0	37.0	33.0	37.0
40	35.04147297690129	37.0	37.0	37.0	27.0	37.0
41	35.2185283608122	37.0	37.0	37.0	33.0	37.0
42	35.25255001633554	37.0	37.0	37.0	33.0	37.0
43	34.91246080113603	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	21.0
16	42.0
17	25.0
18	26.0
19	30.0
20	57.0
21	123.0
22	375.0
23	830.0
24	1778.0
25	3238.0
26	5100.0
27	7928.0
28	10963.0
29	15050.0
30	19317.0
31	24839.0
32	31898.0
33	41913.0
34	59904.0
35	121852.0
36	432133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.052952873897354	20.52862835489539	13.569817067680583	23.848601703526676
2	16.325764104516583	21.381034824283613	38.82790573235955	23.465295338840253
3	17.98632959716816	27.65954162732846	29.827537861150482	24.526590914352894
4	12.138335009762736	17.706310148872078	40.613367359276396	29.54198748208879
5	13.525440995258833	37.09608127123942	35.74897806407133	13.629499669430418
6	29.076360287402597	40.60539252887017	16.958348232546054	13.359898951181176
7	26.69896044226866	32.07425338865979	23.566524234480593	17.660261934590956
8	24.75052929721164	35.02404025488587	20.546250157567215	19.679180290335278
9	26.12927457341089	15.413675033378524	20.29517162606792	38.16187876714267
10	15.552720060300008	27.784437761593733	33.39653172053107	23.266310457575187
11	33.75449870473319	24.242198171963068	23.732066278558253	18.271236844745488
12	23.819146281542384	26.249668787285547	29.17668879896482	20.754496132207255
13	28.09674241040535	22.433969690499406	25.76719154770878	23.702096351386462
14	22.123337183547154	21.150279247690516	28.122210417186533	28.6041731515758
15	24.34844349318152	28.6305415424351	25.000321565742183	22.020693398641193
16	22.708329581733008	28.266529122279877	25.41977191985038	23.605369376136736
17	22.614303758717647	27.305176179438828	25.96347527673948	24.117044785104046
18	22.999925396747813	26.946823316346087	28.293412018326674	21.759839268579427
19	25.886814003802193	25.579011275381188	27.699544405656468	20.83463031516015
20	25.06412020899201	25.62788926819355	27.642305703547255	21.665684819267188
21	23.223735153309683	26.633875536050095	28.010048286311847	22.13234102432838
22	22.678231028264342	26.9939005410022	27.92476905148396	22.40309937924949
23	22.924679013076148	26.591171605487713	28.258682918170525	22.225466463265615
24	22.708329581733008	27.32755715509502	26.889713240533748	23.074400022638226
25	22.98950666670097	27.37592064271988	27.735173889890746	21.89939880068841
26	23.244315360809626	27.096287073314418	27.728613948750137	21.930783617125822
27	23.048160258075804	26.632846525675095	27.345050331469967	22.973942884779134
28	22.484777077764885	26.659986674315643	28.20543163126442	22.649804616655047
29	24.252616902009915	26.78359654561217	26.99904559287719	21.964740959500727
30	23.92243319793272	26.07936757022353	27.64011905650039	22.358080175343368
31	22.782675581326547	26.789256102674656	27.906889996218386	22.52117831978041
32	21.67391690226717	26.71979790236235	28.18459417117073	23.42169102419975
33	21.666713829642188	26.79543016492464	28.65510916513816	22.882746840295017
34	23.403940595231052	26.031132708895537	28.183693787092608	22.381232908780802
35	22.75270565415476	26.90592015393995	27.358170213751183	22.98320397815411
36	23.378858467340496	26.943350406330474	27.48885453137581	22.18893659495322
37	22.979473815544747	26.080911085786024	27.92849921409333	23.011115884575904
38	23.418989871965383	26.439006696285016	27.309163594641944	22.832839837107656
39	22.8453165879045	26.530974498550382	27.766172827437536	22.857536086107586
40	22.66768367192062	27.134231830892436	27.929528224468324	22.26855627271862
41	22.512174478999185	25.96051687191136	27.907919006593385	23.61938964249607
42	22.033556028328654	27.17745026664231	27.82611268178111	22.962881023247917
43	23.375771436215505	26.270377621082364	27.43328797112597	22.92056297157616
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	60.5
2	79.0
3	278.5
4	478.0
5	478.0
6	574.0
7	670.0
8	615.0
9	560.0
10	739.0
11	918.0
12	918.0
13	1506.0
14	2094.0
15	3755.0
16	5416.0
17	6178.5
18	6941.0
19	6941.0
20	6445.0
21	5949.0
22	6302.5
23	6656.0
24	8576.0
25	10496.0
26	10496.0
27	13245.0
28	15994.0
29	18768.0
30	21542.0
31	25557.0
32	29572.0
33	29572.0
34	35652.0
35	41732.0
36	45383.0
37	49034.0
38	52021.5
39	55009.0
40	55009.0
41	56944.5
42	58880.0
43	60125.0
44	61370.0
45	60248.5
46	59127.0
47	59127.0
48	57878.0
49	56629.0
50	56126.5
51	55624.0
52	52869.5
53	50115.0
54	50115.0
55	48692.5
56	47270.0
57	40596.5
58	33923.0
59	32339.5
60	30756.0
61	30756.0
62	27857.5
63	24959.0
64	20821.0
65	16683.0
66	13815.0
67	10947.0
68	10947.0
69	9362.5
70	7778.0
71	6588.5
72	5399.0
73	3779.5
74	2160.0
75	2160.0
76	1588.5
77	1017.0
78	802.0
79	587.0
80	484.5
81	382.0
82	382.0
83	325.0
84	268.0
85	222.5
86	177.0
87	151.0
88	125.0
89	125.0
90	91.0
91	57.0
92	42.5
93	28.0
94	15.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	777446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.43698256784095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.53000205582589	44.147491878527724
2	6.9016268453908065	6.961944657814385
3	1.6886140397702378	2.5550579066310886
4	0.7948714597982194	1.603636718460683
5	0.4926325588323422	1.242344989108886
6	0.3327918297040345	1.0071009428101367
7	0.25932685922784554	0.9155764994773451
8	0.21417525031483553	0.8641882693273863
9	0.1690475540398646	0.7673623682620322
>10	1.2678390836953335	12.98099967576485
>50	0.18880001170241342	6.688118618800252
>100	0.14626806271485757	14.030511054114706
>500	0.011410983616044097	3.8819874987306378
>1k	0.0025934053672827494	2.3536789221699275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4183	0.5380437998266118	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2982	0.3835636172801712	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1919	0.2468338637024308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1665	0.21416278429627267	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1408	0.18110582599949063	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1320	0.1697867118745225	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1168	0.15023551474957747	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1162	0.14946375696832961	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1098	0.1412316739683528	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1089	0.14007403729648105	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	962	0.12373849759340198	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	926	0.11910795090591501	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	892	0.11473465681217732	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	857	0.11023273642156498	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	843	0.10843196826532003	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	826	0.10624532121845118	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	803	0.1032869163903345	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	799	0.10277241120283595	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	789	0.10148614823408957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.572525937492765E-4	0.0	0.0
2	1.2862629687463825E-4	0.0	2.572525937492765E-4	0.0	0.0
3	1.2862629687463825E-4	0.0	2.572525937492765E-4	0.0	0.0
4	1.2862629687463825E-4	0.0	5.14505187498553E-4	2.572525937492765E-4	0.0
5	1.2862629687463825E-4	0.0	9.003840781224676E-4	2.572525937492765E-4	0.0
6	1.2862629687463825E-4	0.0	0.0014148892656210205	3.858788906239147E-4	0.0
7	1.2862629687463825E-4	0.0	0.0029584048281166794	3.858788906239147E-4	0.0
8	1.2862629687463825E-4	0.0	0.0033442837187405944	3.858788906239147E-4	0.0
9	1.2862629687463825E-4	0.0	0.0033442837187405944	0.0012862629687463824	0.0
10	1.2862629687463825E-4	0.0	0.004630546687486976	0.0014148892656210205	0.0
11	1.2862629687463825E-4	0.0	0.006688567437481189	0.0014148892656210205	0.0
12	1.2862629687463825E-4	0.0	0.007460325218729018	0.0015435155624956589	0.0
13	1.2862629687463825E-4	0.0	0.007846204109352932	0.0016721418593702972	0.0
14	1.2862629687463825E-4	0.0	0.008617961890600762	0.0018007681562449353	0.0
15	1.2862629687463825E-4	0.0	0.009261093374973953	0.0019293944531195736	0.0
16	1.2862629687463825E-4	0.0	0.010547356343720336	0.002058020749994212	0.0
17	1.2862629687463825E-4	0.0	0.01106186153121889	0.00218664704686885	0.0
18	2.572525937492765E-4	0.0	0.01106186153121889	0.00218664704686885	0.0
19	2.572525937492765E-4	0.0	0.011447740421842803	0.0024438996406181265	0.0
20	2.572525937492765E-4	0.0	0.011447740421842803	0.002701152234367403	0.0
21	2.572525937492765E-4	0.0	0.01170499301559208	0.002829778531242041	0.0
22	2.572525937492765E-4	0.0	0.011833619312466718	0.003215657421865956	0.0
23	2.572525937492765E-4	0.0	0.011833619312466718	0.003730162609364509	0.0
24	2.572525937492765E-4	0.0	0.011833619312466718	0.004501920390612338	0.0
25	2.572525937492765E-4	0.0	0.011833619312466718	0.004759172984361615	0.0
26	2.572525937492765E-4	0.0	0.011833619312466718	0.004887799281236253	0.0
27	3.858788906239147E-4	0.0	0.011833619312466718	0.006174062249982635	0.0
28	3.858788906239147E-4	0.0	0.011833619312466718	0.014020266359335568	0.0
29	3.858788906239147E-4	0.0	0.011833619312466718	0.03125619014053709	0.0
30	3.858788906239147E-4	0.0	0.011833619312466718	0.05775320729671257	0.0
31	3.858788906239147E-4	0.0	0.011962245609341357	0.12296673981215415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	25	0.005495264	29.599998	15
AACGGTT	35	8.8668027E-4	26.42857	6
GGTATCA	805	0.0	25.04969	1
GTATCAA	2250	0.0	23.26889	1
TTAGAGT	40	0.0019306282	23.125	4
AAACGGA	40	0.0019306282	23.125	32
CGTCCTA	40	0.0019306282	23.125	33
GCTTTAT	420	0.0	20.702381	1
AACGGAG	45	0.0038247104	20.555557	33
TATCGGA	45	0.0038247104	20.555557	26
CGCTCTC	145	0.0	20.413794	29
AACCACG	65	6.898459E-5	19.923077	35
TACTAGG	65	6.898459E-5	19.923077	2
TGCGGTA	75	9.259444E-6	19.733334	36
GCCGCTC	150	0.0	19.733334	27
TTGCGGT	75	9.259444E-6	19.733334	35
GCTCGGA	360	0.0	19.527779	11
CCGGCAG	155	1.8189894E-12	19.096773	16
GCCGGCA	165	0.0	19.060606	15
TGGGGCG	175	0.0	19.028572	37
>>END_MODULE