FastQCFastQC Report
Fri 10 Feb 2017
ERR1633154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928551
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45150.48624146654303324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.3206070533551738No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC20380.21948175167545997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17550.18900415809147803No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12090.13020286446301818No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11490.12374118384450611No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11220.12083342756617568No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11020.11867953402667165No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10810.11641794581019245No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10030.10801776100612674No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9940.10704850891334994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.030.6643831
GTATCAA23250.025.5419351
TTAACGG2700.022.61111335
TTTAACG2900.021.05172334
CGAGTCG1500.020.96666721
GTTTAAC3200.020.812533
AACGGCC3000.020.3537
GCCGTCC1750.020.08571427
GTGACAC3200.019.6562524
AGGTCGC2450.018.8775535
GTCGCCC2400.018.537
CGAACTA3000.018.529
CGTAGCG500.00703388918.515
GCTAGAC500.00703388918.53
GCGGAAT609.234995E-418.519
CACATGT3550.018.23943728
GACACAT3600.017.9861126
TAACGGC3450.017.69565236
GCACCGC3450.017.69565210
GCGAACT3150.017.61904728
Produced by FastQC (version 0.11.5)