##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633154.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	928551
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.765266528171313	33.0	33.0	33.0	27.0	33.0
2	31.89923870632846	33.0	33.0	33.0	33.0	33.0
3	31.618030673597897	33.0	33.0	33.0	27.0	33.0
4	31.91869805751111	33.0	33.0	33.0	33.0	33.0
5	32.00889450337138	33.0	33.0	33.0	33.0	33.0
6	35.145844439346895	37.0	37.0	37.0	33.0	37.0
7	35.295322497094936	37.0	37.0	37.0	33.0	37.0
8	35.414271267814044	37.0	37.0	37.0	33.0	37.0
9	35.542866250749825	37.0	37.0	37.0	33.0	37.0
10	35.546370635538594	37.0	37.0	37.0	33.0	37.0
11	35.55056211236647	37.0	37.0	37.0	33.0	37.0
12	35.549250391200914	37.0	37.0	37.0	33.0	37.0
13	35.55134182182777	37.0	37.0	37.0	33.0	37.0
14	35.53420759872102	37.0	37.0	37.0	33.0	37.0
15	35.547072804832474	37.0	37.0	37.0	33.0	37.0
16	35.540629432309046	37.0	37.0	37.0	33.0	37.0
17	35.52327981984834	37.0	37.0	37.0	33.0	37.0
18	35.51321036755116	37.0	37.0	37.0	33.0	37.0
19	35.49417425644903	37.0	37.0	37.0	33.0	37.0
20	35.49585106256953	37.0	37.0	37.0	33.0	37.0
21	35.50516449823434	37.0	37.0	37.0	33.0	37.0
22	35.40439566593542	37.0	37.0	37.0	33.0	37.0
23	35.453304126536935	37.0	37.0	37.0	33.0	37.0
24	35.43486572089201	37.0	37.0	37.0	33.0	37.0
25	35.45564648576115	37.0	37.0	37.0	33.0	37.0
26	35.355488282280675	37.0	37.0	37.0	33.0	37.0
27	35.355411819060016	37.0	37.0	37.0	33.0	37.0
28	35.38529170718679	37.0	37.0	37.0	33.0	37.0
29	35.380479909019535	37.0	37.0	37.0	33.0	37.0
30	35.38078899274245	37.0	37.0	37.0	33.0	37.0
31	35.37145078730194	37.0	37.0	37.0	33.0	37.0
32	35.37823339805784	37.0	37.0	37.0	33.0	37.0
33	35.37080138839978	37.0	37.0	37.0	33.0	37.0
34	35.339013150597005	37.0	37.0	37.0	33.0	37.0
35	35.286603536047025	37.0	37.0	37.0	33.0	37.0
36	35.31138085037871	37.0	37.0	37.0	33.0	37.0
37	35.3061899669485	37.0	37.0	37.0	33.0	37.0
38	35.290672240943145	37.0	37.0	37.0	33.0	37.0
39	35.19629293382916	37.0	37.0	37.0	33.0	37.0
40	34.99145011959494	37.0	37.0	37.0	27.0	37.0
41	35.1599825965402	37.0	37.0	37.0	33.0	37.0
42	35.20264368893039	37.0	37.0	37.0	33.0	37.0
43	34.855302508962886	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	32.0
16	40.0
17	30.0
18	29.0
19	37.0
20	70.0
21	189.0
22	487.0
23	1105.0
24	2408.0
25	4266.0
26	6602.0
27	10078.0
28	13774.0
29	18682.0
30	24142.0
31	30663.0
32	39237.0
33	51329.0
34	72986.0
35	147220.0
36	505139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24883716672536	19.565753523500597	13.304061920131474	24.88134738964257
2	16.73747591677786	20.971815226088818	38.68705111512453	23.603657742008785
3	18.435820972676783	26.763634953815135	29.25030504517253	25.550239028335547
4	12.78820441742026	17.43835287453247	38.99731947949009	30.776123228557182
5	14.319622724007623	36.62835967006659	34.72701014806941	14.325007457856382
6	30.307220605007156	39.09198310055129	16.843339784244485	13.75745651019707
7	27.59406860797091	31.72911342511074	22.61340518722181	18.063412779696538
8	25.15069177675755	35.088110399967256	20.07461087220842	19.68658695106677
9	26.825451698398904	14.8577730248527	19.498552045068067	38.81822323168033
10	15.966920503020296	27.341632285141042	32.8462303093745	23.845216902464163
11	34.736594974320205	23.526656047971517	23.09975434844182	18.63699462926646
12	24.0387442369886	25.29823348421358	29.109117323658047	21.55390495513978
13	28.630414484503277	21.60624456814973	25.57931659111885	24.184024356228147
14	22.70677647215931	20.45875778497896	27.35444795170109	29.480017791160634
15	24.98311885938414	27.644038938087405	24.236040885207167	23.13680131732129
16	23.277558260127876	27.43726515829502	24.620295492654684	24.66488108892242
17	23.012952438799807	26.734126612323934	25.58696291318409	24.66595803569217
18	23.924587879394885	25.975202223679688	27.14573566772315	22.954474229202273
19	25.81506023901757	25.20120058025892	26.703864408093903	22.27987477262961
20	25.17793853003227	25.33226500213774	26.840205869144505	22.649590598685478
21	24.33608924011713	26.01806470511582	26.72314175527246	22.92270429949459
22	23.846940017295765	25.997064243105655	26.655509498132034	23.500486241466543
23	23.669889968348535	25.97434066626389	27.18127491112497	23.17449445426261
24	23.818293233220363	26.296670834450666	26.082573816623967	23.802462115705005
25	23.841555283447004	26.072019738280396	27.3609096323196	22.725515345952996
26	24.059637004321786	26.090004749335254	27.09727306308431	22.753085183258648
27	24.080422076978	26.03809591503321	26.145359813300505	23.736122194688285
28	23.21821849311454	25.77478242982884	27.424557186411946	23.58244189064467
29	24.639357450479295	25.658364484018648	26.389934424711193	23.312343640790868
30	24.180147347857037	25.643825702626998	27.060764567589718	23.11526238192625
31	23.9731581787107	25.627563806403742	26.92722316814047	23.472054846745092
32	22.71194581665412	25.660410682881178	27.376309971127057	24.251333529337646
33	23.10600063970638	25.885384863082372	27.418418589824363	23.590195907386885
34	23.71619867944787	25.67925725135184	27.221660414990666	23.382883654209625
35	23.861155714656494	26.09969727026302	26.766542710093468	23.27260430498702
36	24.516800908081514	25.895615857395015	26.22591543167796	23.36166780284551
37	23.806339124076114	25.705642447210764	26.577861636032917	23.91015679268021
38	24.25122583466067	25.81053706258461	26.37604181138139	23.562195291373335
39	23.21961852391522	25.926739619040852	26.97762427696486	23.876017580079072
40	23.67538239687427	26.147513706840016	27.273569249292716	22.903534646993005
41	23.487132101521617	25.22370876774674	27.172013168905103	24.117145961826544
42	22.827394510371533	26.57818472006384	26.8515138102269	23.74290695933772
43	23.96981964372447	25.19829282398059	26.94337736968675	23.888510162608192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	40.5
2	49.0
3	222.5
4	396.0
5	396.0
6	503.5
7	611.0
8	569.0
9	527.0
10	738.0
11	949.0
12	949.0
13	1510.5
14	2072.0
15	3671.5
16	5271.0
17	6090.0
18	6909.0
19	6909.0
20	6099.0
21	5289.0
22	5279.5
23	5270.0
24	7083.5
25	8897.0
26	8897.0
27	11650.0
28	14403.0
29	17800.5
30	21198.0
31	25445.0
32	29692.0
33	29692.0
34	36372.5
35	43053.0
36	47342.0
37	51631.0
38	56104.5
39	60578.0
40	60578.0
41	64442.0
42	68306.0
43	70389.0
44	72472.0
45	72462.5
46	72453.0
47	72453.0
48	70798.0
49	69143.0
50	69815.5
51	70488.0
52	68537.5
53	66587.0
54	66587.0
55	65080.0
56	63573.0
57	56376.5
58	49180.0
59	44713.0
60	40246.0
61	40246.0
62	36640.5
63	33035.0
64	26280.5
65	19526.0
66	16653.5
67	13781.0
68	13781.0
69	11638.5
70	9496.0
71	8238.0
72	6980.0
73	7281.0
74	7582.0
75	7582.0
76	6350.5
77	5119.0
78	3714.0
79	2309.0
80	1481.5
81	654.0
82	654.0
83	511.5
84	369.0
85	296.5
86	224.0
87	176.5
88	129.0
89	129.0
90	94.0
91	59.0
92	35.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	928551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.168018185958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.30195700001458	42.79430130074539
2	8.425754928231004	8.454068529398363
3	2.0960102736981217	3.154580445865265
4	0.9273556989152677	1.8609439027213175
5	0.5700425805165134	1.4298953273061434
6	0.3857971137836945	1.1612805972234295
7	0.2670773963011296	0.9379120572285367
8	0.2175652540680212	0.8731854098173647
9	0.1531348224939177	0.6914223503800481
>10	1.3135595133281415	13.460196582497852
>50	0.18432029348974352	6.513680589299835
>100	0.14649282480379486	13.896672801628164
>500	0.008745840284868259	2.802808298868078
>1k	0.0021864600712170647	1.9690518070202279
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4515	0.48624146654303324	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2977	0.3206070533551738	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2038	0.21948175167545997	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1755	0.18900415809147803	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1209	0.13020286446301818	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1149	0.12374118384450611	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1122	0.12083342756617568	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1102	0.11867953402667165	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1081	0.11641794581019245	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1003	0.10801776100612674	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	994	0.10704850891334994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.2308403092560345E-4	0.0	0.0
2	0.0	0.0	3.2308403092560345E-4	0.0	0.0
3	0.0	0.0	5.384733848760058E-4	1.0769467697520115E-4	0.0
4	0.0	0.0	7.53862738826408E-4	2.153893539504023E-4	0.0
5	2.153893539504023E-4	0.0	0.0011846414467272127	4.307787079008046E-4	0.0
6	2.153893539504023E-4	0.0	0.0017231148316032183	4.307787079008046E-4	0.0
7	2.153893539504023E-4	0.0	0.004630871109933649	6.461680618512069E-4	0.0
8	2.153893539504023E-4	0.0	0.005923207233636063	6.461680618512069E-4	0.0
9	2.153893539504023E-4	0.0	0.007000154003388074	0.0020461988625288217	0.0
10	4.307787079008046E-4	0.0	0.00850787948104089	0.002261588216479224	0.0
11	4.307787079008046E-4	0.0	0.012600277206098534	0.00280006160135523	0.0
12	4.307787079008046E-4	0.0	0.013354139944924941	0.00280006160135523	0.0
13	4.307787079008046E-4	0.0	0.014538781391652154	0.00280006160135523	0.0
14	4.307787079008046E-4	0.0	0.01583111751535457	0.002907756278330431	0.0
15	4.307787079008046E-4	0.0	0.01712345363905698	0.003123145632280833	0.0
16	4.307787079008046E-4	0.0	0.017985011054858592	0.003123145632280833	0.0
17	5.384733848760058E-4	0.0	0.018415789762759396	0.003123145632280833	0.0
18	6.461680618512069E-4	0.0	0.0188465684706602	0.0032308403092560344	0.0
19	6.461680618512069E-4	0.0	0.019061957824610603	0.0032308403092560344	0.0
20	6.461680618512069E-4	0.0	0.01960043120948661	0.0037693136941320403	0.0
21	6.461680618512069E-4	0.0	0.020031209917387414	0.0040923977250576435	0.0
22	6.461680618512069E-4	0.0	0.020461988625288217	0.004846260463884052	0.0
23	6.461680618512069E-4	0.0	0.02067737797923862	0.005169344494809655	0.0
24	6.461680618512069E-4	0.0	0.02078507265621382	0.005384733848760057	0.0
25	6.461680618512069E-4	0.0	0.02089276733318902	0.005492428525735259	0.0
26	6.461680618512069E-4	0.0	0.02089276733318902	0.005707817879685661	0.0
27	6.461680618512069E-4	0.0	0.02089276733318902	0.00656937529548727	0.0
28	6.461680618512069E-4	0.0	0.02089276733318902	0.013892613329800949	0.0
29	6.461680618512069E-4	0.0	0.02089276733318902	0.029723730845155515	0.0
30	6.461680618512069E-4	0.0	0.02089276733318902	0.05567814799617899	0.0
31	6.461680618512069E-4	0.0	0.02089276733318902	0.1221257636898781	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	730	0.0	30.664383	1
GTATCAA	2325	0.0	25.541935	1
TTAACGG	270	0.0	22.611113	35
TTTAACG	290	0.0	21.051723	34
CGAGTCG	150	0.0	20.966667	21
GTTTAAC	320	0.0	20.8125	33
AACGGCC	300	0.0	20.35	37
GCCGTCC	175	0.0	20.085714	27
GTGACAC	320	0.0	19.65625	24
AGGTCGC	245	0.0	18.87755	35
GTCGCCC	240	0.0	18.5	37
CGAACTA	300	0.0	18.5	29
CGTAGCG	50	0.007033889	18.5	15
GCTAGAC	50	0.007033889	18.5	3
GCGGAAT	60	9.234995E-4	18.5	19
CACATGT	355	0.0	18.239437	28
GACACAT	360	0.0	17.98611	26
TAACGGC	345	0.0	17.695652	36
GCACCGC	345	0.0	17.695652	10
GCGAACT	315	0.0	17.619047	28
>>END_MODULE