FastQCFastQC Report
Fri 10 Feb 2017
ERR1633153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855374
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43290.5060944101644427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29450.3442938410566606No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC28560.33388903567328443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17050.19932801324332983No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15320.17910294210485705No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14120.16507399102614762No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13820.16156675325647027No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12200.14262766930021253No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11770.13760062849700833No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9460.11059489767049269No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9210.10767219952909488No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9150.1069707519751594No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG8990.1051002251646648No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT8900.10404805383376162No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8670.10135917154367564No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8660.10124226361801973No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8640.10100844776670789No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8640.10100844776670789No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA405.9383812E-527.754
AACGATT901.4188117E-1024.66666822
GGTATCA8850.024.6666661
GTATCAA24850.024.0462781
TTAACGG3750.021.21333335
AACGGCC3850.020.6623437
CTTAGAC450.003825019320.5555573
TAACGGC3950.020.1392436
GTTTAAC4150.020.06024233
TGCGGGT1403.6379788E-1219.82142821
CGAGTTC951.6748527E-719.47368414
TTTAACG4100.019.40243734
CCGAGTT1052.2573658E-819.3809513
GGTAACG1551.8189894E-1219.09677525
ATTAGAG4950.019.0606063
CACATGT4500.018.91111228
GTATTAG6900.018.51
TTAGACT609.234181E-418.54
TATTAGA5200.018.52
CTCTAAT2500.018.51
Produced by FastQC (version 0.11.5)