##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855374
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812990574883035	33.0	33.0	33.0	27.0	33.0
2	31.92660403519396	33.0	33.0	33.0	33.0	33.0
3	31.652588224566095	33.0	33.0	33.0	27.0	33.0
4	31.939551587960356	33.0	33.0	33.0	33.0	33.0
5	32.0344176933131	33.0	33.0	33.0	33.0	33.0
6	35.178111562895296	37.0	37.0	37.0	33.0	37.0
7	35.32530799393014	37.0	37.0	37.0	33.0	37.0
8	35.46379478450362	37.0	37.0	37.0	33.0	37.0
9	35.57606614182802	37.0	37.0	37.0	33.0	37.0
10	35.579159525540874	37.0	37.0	37.0	33.0	37.0
11	35.6044993184268	37.0	37.0	37.0	33.0	37.0
12	35.58648731432099	37.0	37.0	37.0	33.0	37.0
13	35.5871139408025	37.0	37.0	37.0	33.0	37.0
14	35.57059251274881	37.0	37.0	37.0	33.0	37.0
15	35.58714199870466	37.0	37.0	37.0	33.0	37.0
16	35.57513087842277	37.0	37.0	37.0	33.0	37.0
17	35.56402813272323	37.0	37.0	37.0	33.0	37.0
18	35.55554646271689	37.0	37.0	37.0	33.0	37.0
19	35.541480101101975	37.0	37.0	37.0	33.0	37.0
20	35.551570424165334	37.0	37.0	37.0	33.0	37.0
21	35.54289117976464	37.0	37.0	37.0	33.0	37.0
22	35.44830682251273	37.0	37.0	37.0	33.0	37.0
23	35.496896094573835	37.0	37.0	37.0	33.0	37.0
24	35.49019960859226	37.0	37.0	37.0	33.0	37.0
25	35.5098307874684	37.0	37.0	37.0	33.0	37.0
26	35.411290266012294	37.0	37.0	37.0	33.0	37.0
27	35.410895117223575	37.0	37.0	37.0	33.0	37.0
28	35.43918215891528	37.0	37.0	37.0	33.0	37.0
29	35.43841290476447	37.0	37.0	37.0	33.0	37.0
30	35.42955011492049	37.0	37.0	37.0	33.0	37.0
31	35.422224664298895	37.0	37.0	37.0	33.0	37.0
32	35.42338789815917	37.0	37.0	37.0	33.0	37.0
33	35.41722334323933	37.0	37.0	37.0	33.0	37.0
34	35.404770310998465	37.0	37.0	37.0	33.0	37.0
35	35.34345678030896	37.0	37.0	37.0	33.0	37.0
36	35.3637823922635	37.0	37.0	37.0	33.0	37.0
37	35.3706448874995	37.0	37.0	37.0	33.0	37.0
38	35.3594416009839	37.0	37.0	37.0	33.0	37.0
39	35.254425549525706	37.0	37.0	37.0	33.0	37.0
40	35.0472413236783	37.0	37.0	37.0	27.0	37.0
41	35.21802159055571	37.0	37.0	37.0	33.0	37.0
42	35.258671645385526	37.0	37.0	37.0	33.0	37.0
43	34.93473147418556	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	28.0
16	34.0
17	34.0
18	26.0
19	24.0
20	46.0
21	171.0
22	411.0
23	941.0
24	2021.0
25	3525.0
26	5772.0
27	8816.0
28	12484.0
29	16919.0
30	21723.0
31	27640.0
32	35151.0
33	46164.0
34	66270.0
35	132000.0
36	475174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02851618122599	19.55483800068742	13.52881897275344	24.88782684533315
2	16.673174541194847	20.71246028053226	39.01638347670142	23.597981701571477
3	18.0723285954448	26.942717454587118	29.34073282564118	25.644221124326904
4	12.676209470944874	17.292903455096834	39.08945093023636	30.94143614372193
5	14.053735558948482	36.90935193260492	34.73287708066881	14.304035427777793
6	30.39886646075284	39.112364883664924	16.764713446983425	13.724055208598813
7	27.567590317217967	31.736643854033442	22.65897724270319	18.0367885860454
8	25.189215477674093	35.0481777561628	19.882647824226595	19.87995894193651
9	26.882860596651287	14.997065611066038	19.47066429421516	38.64940949806751
10	16.16766467065868	27.237559243091326	32.622338298802624	23.97243778744736
11	34.40085857180601	23.600553675935906	23.22294107606731	18.775646676190767
12	24.516877997226942	25.38398408181684	28.63390750712554	21.465230413830675
13	28.0586036049728	21.83313965586983	25.598393217469784	24.50986352168759
14	22.69650468683874	20.44228606434145	27.32231748919186	29.53889175962795
15	25.060149127749966	27.345699074323043	24.695513307629177	22.898638490297813
16	23.42484106367507	27.362533815617496	24.462866535573912	24.74975858513352
17	22.899690661628714	26.76256234115136	25.437411003841593	24.900335993378338
18	23.704367913918357	26.071987224301886	27.361481644286595	22.862163217493165
19	26.082859661387886	24.91962580111156	26.565806302272456	22.431708235228097
20	25.017594642811215	25.355224732105487	27.128016516751735	22.49916410833156
21	24.393657043585613	26.053515772048254	26.78442412324901	22.768403061117127
22	23.74037555502038	26.115710788497193	26.567326105305984	23.576587551176445
23	23.64708303034696	26.112904998281454	27.186704295431003	23.05330767594058
24	24.083383408894825	26.476254831220025	25.691919557994513	23.748442201890636
25	23.826536696228786	26.01867721020279	27.252055825872663	22.902730267695766
26	24.0829157771922	26.17977633175664	27.202369957468896	22.53493793358227
27	24.2836466855434	26.02428879063427	25.927372120265524	23.764692403556804
28	23.076221629369144	25.675903172179655	27.68753784894093	23.56033734951027
29	24.94429337342496	25.46757324866082	26.482684767131104	23.10544861078312
30	24.178312644527423	25.403156981624413	27.36732704056939	23.051203333278778
31	23.63597677740965	25.720328183928903	27.2469118771438	23.39678316151765
32	22.48151101155752	25.472600289464026	27.403919221299688	24.641969477678767
33	23.043838133962456	26.008973852373344	27.525620371907493	23.421567641756706
34	23.73032147341397	25.888792504799067	27.210085880562186	23.170800141224774
35	24.015927535791363	26.21823903929743	26.697678442412325	23.068154982498886
36	24.664766523181672	25.98664443857307	26.074559198666314	23.274029839578944
37	23.625571972026272	25.69706350672337	26.629638029680585	24.047726491569772
38	24.430833763944193	25.884233095698487	26.25775391816913	23.427179222188187
39	23.005375426421658	26.18620626766771	26.992052599213913	23.816365706696722
40	23.614582627014617	26.312233011524782	27.22317956823565	22.85000479322495
41	23.174775010697076	25.266374708606996	27.37422460818309	24.18462567251284
42	22.76033641424687	26.633495991227228	26.8801717143612	23.7259958801647
43	23.96425423265145	24.854858810298186	27.17968981989165	24.001197137158716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	45.5
2	58.0
3	236.0
4	414.0
5	414.0
6	525.0
7	636.0
8	582.5
9	529.0
10	724.0
11	919.0
12	919.0
13	1484.5
14	2050.0
15	3640.5
16	5231.0
17	5928.5
18	6626.0
19	6626.0
20	5746.5
21	4867.0
22	4962.0
23	5057.0
24	6625.5
25	8194.0
26	8194.0
27	10495.0
28	12796.0
29	15697.5
30	18599.0
31	22838.5
32	27078.0
33	27078.0
34	33930.5
35	40783.0
36	44877.5
37	48972.0
38	53211.5
39	57451.0
40	57451.0
41	60971.0
42	64491.0
43	66286.0
44	68081.0
45	66621.5
46	65162.0
47	65162.0
48	62730.0
49	60298.0
50	61513.0
51	62728.0
52	61373.0
53	60018.0
54	60018.0
55	60570.5
56	61123.0
57	53090.0
58	45057.0
59	40316.5
60	35576.0
61	35576.0
62	32777.0
63	29978.0
64	23932.0
65	17886.0
66	14746.5
67	11607.0
68	11607.0
69	9992.0
70	8377.0
71	7502.5
72	6628.0
73	7360.0
74	8092.0
75	8092.0
76	7012.0
77	5932.0
78	4278.0
79	2624.0
80	1609.0
81	594.0
82	594.0
83	457.5
84	321.0
85	302.0
86	283.0
87	211.0
88	139.0
89	139.0
90	103.0
91	67.0
92	41.5
93	16.0
94	9.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	855374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.168372342369594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.02418091983841	39.25428042812096
2	8.165632090883554	7.539878855654276
3	2.2015900088512264	3.0493148182165255
4	1.0232270617672516	1.889629119138371
5	0.5839256487106796	1.3479448384967179
6	0.41063002184122444	1.1374871845992605
7	0.2827709083890315	0.913855081026642
8	0.2379880836027263	0.879001796545412
9	0.19271304943111112	0.8007523039232113
>10	1.4804871921470024	13.909855998764655
>50	0.20165511603486316	6.546743101765995
>100	0.17738403475066486	15.542110829748212
>500	0.015492055436697072	4.976899095260844
>1k	0.0023238083155045605	2.21224654873894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4329	0.5060944101644427	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2945	0.3442938410566606	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2856	0.33388903567328443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1705	0.19932801324332983	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1532	0.17910294210485705	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1412	0.16507399102614762	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1382	0.16156675325647027	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1220	0.14262766930021253	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1177	0.13760062849700833	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	946	0.11059489767049269	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	921	0.10767219952909488	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	915	0.1069707519751594	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	899	0.1051002251646648	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	890	0.10404805383376162	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	867	0.10135917154367564	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	866	0.10124226361801973	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	864	0.10100844776670789	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	864	0.10100844776670789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.5072377696773575E-4	0.0	0.0
2	0.0	0.0	3.5072377696773575E-4	0.0	0.0
3	0.0	0.0	5.845396282795596E-4	0.0	0.0
4	0.0	0.0	7.014475539354715E-4	0.0	0.0
5	0.0	0.0	8.183554795913834E-4	0.0	0.0
6	0.0	0.0	0.0010521713309032071	0.0	0.0
7	0.0	0.0	0.0022212505874623263	0.0	0.0
8	0.0	0.0	0.002805790215741886	0.0	0.0
9	0.0	0.0	0.0033903298440214457	7.014475539354715E-4	0.0
10	1.1690792565591192E-4	0.0	0.0035072377696773573	7.014475539354715E-4	1.1690792565591192E-4
11	1.1690792565591192E-4	0.0	0.006663751762386979	7.014475539354715E-4	1.1690792565591192E-4
12	1.1690792565591192E-4	0.0	0.007599015167634274	8.183554795913834E-4	1.1690792565591192E-4
13	1.1690792565591192E-4	0.0	0.007832831018946098	9.352634052472953E-4	1.1690792565591192E-4
14	1.1690792565591192E-4	0.0	0.008417370647225657	9.352634052472953E-4	1.1690792565591192E-4
15	1.1690792565591192E-4	0.0	0.009352634052472953	0.0011690792565591192	1.1690792565591192E-4
16	2.3381585131182383E-4	0.0	0.010521713309032073	0.001285987182215031	1.1690792565591192E-4
17	2.3381585131182383E-4	0.0	0.01098934501165572	0.001285987182215031	1.1690792565591192E-4
18	2.3381585131182383E-4	0.0	0.011456976714279367	0.0015198030335268548	2.3381585131182383E-4
19	2.3381585131182383E-4	0.0	0.011690792565591192	0.0016367109591827668	2.3381585131182383E-4
20	2.3381585131182383E-4	0.0	0.011807700491247103	0.0017536188848386786	2.3381585131182383E-4
21	2.3381585131182383E-4	0.0	0.012275332193870751	0.0022212505874623263	3.5072377696773575E-4
22	2.3381585131182383E-4	0.0	0.012509148045182576	0.002805790215741886	3.5072377696773575E-4
23	2.3381585131182383E-4	0.0	0.012626055970838487	0.003974869472301005	3.5072377696773575E-4
24	2.3381585131182383E-4	0.0	0.012859871822150312	0.004793224951892388	3.5072377696773575E-4
25	2.3381585131182383E-4	0.0	0.012859871822150312	0.005260856654516036	3.5072377696773575E-4
26	2.3381585131182383E-4	0.0	0.012976779747806222	0.005962304208451508	3.5072377696773575E-4
27	2.3381585131182383E-4	0.0	0.013093687673462135	0.007014475539354715	3.5072377696773575E-4
28	2.3381585131182383E-4	0.0	0.013093687673462135	0.015314938260924461	3.5072377696773575E-4
29	2.3381585131182383E-4	0.0	0.013093687673462135	0.03133132407578439	3.5072377696773575E-4
30	2.3381585131182383E-4	0.0	0.013210595599118047	0.06114284511804193	3.5072377696773575E-4
31	2.3381585131182383E-4	0.0	0.013210595599118047	0.12287022986436343	3.5072377696773575E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	40	5.9383812E-5	27.75	4
AACGATT	90	1.4188117E-10	24.666668	22
GGTATCA	885	0.0	24.666666	1
GTATCAA	2485	0.0	24.046278	1
TTAACGG	375	0.0	21.213333	35
AACGGCC	385	0.0	20.66234	37
CTTAGAC	45	0.0038250193	20.555557	3
TAACGGC	395	0.0	20.13924	36
GTTTAAC	415	0.0	20.060242	33
TGCGGGT	140	3.6379788E-12	19.821428	21
CGAGTTC	95	1.6748527E-7	19.473684	14
TTTAACG	410	0.0	19.402437	34
CCGAGTT	105	2.2573658E-8	19.38095	13
GGTAACG	155	1.8189894E-12	19.096775	25
ATTAGAG	495	0.0	19.060606	3
CACATGT	450	0.0	18.911112	28
GTATTAG	690	0.0	18.5	1
TTAGACT	60	9.234181E-4	18.5	4
TATTAGA	520	0.0	18.5	2
CTCTAAT	250	0.0	18.5	1
>>END_MODULE