FastQCFastQC Report
Fri 10 Feb 2017
ERR1633152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660563
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88701.3427939500093102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.9729579162017854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35600.5389342121796105No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA27540.41691708436591207No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT23270.3522752561072903No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22340.33819635674417126No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG21510.3256313175276242No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC18700.28309184740895266No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17820.2697698781191196No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA16380.24797029200848367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.22329437161936108No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14180.21466536878390102No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12970.1963476610103805No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC12750.1930171686879223No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12600.19074637846806436No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12310.18635618404300575No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11890.1799979714274036No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11170.16909817837208563No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11170.16909817837208563No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10790.16334550981511226No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10770.16304273778579786No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10410.15759284125813888No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10300.15592759509690976No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10290.15577620908225256No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10180.15411096292102344No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9780.14805552233473568No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9670.14639027617350656No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9600.1453305740709062No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9480.14351394189501984No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9450.14305978385104826No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.13579325514750296No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8930.13518771108887417No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8870.13427939500093103No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8800.13321969289833066No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8750.1324627628250447No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC8590.13004058659052958No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8550.12943504253190083No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8520.12898088448792924No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC8390.12701286629738573No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8370.1267100942680713No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8350.12640732223875695No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8240.1247420760775278No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8120.12292544390164148No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG8050.12186574179904111No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG7870.11914079353521163No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC7860.11898940752055444No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7710.11671861730069652No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7700.11656723128603934No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA7690.11641584527138216No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7510.11369089700755265No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7290.1103604046850944No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7290.1103604046850944No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7190.10884654453852244No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7070.10702991236263612No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6980.10566743823072137No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6940.1050618941720926No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6790.10279110395223469No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6630.10036892771771957No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6630.10036892771771957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT250.00549467229.635
GGTATCA17150.027.9387761
GGACGTA1400.023.78571327
CCGTGCA1103.6379788E-1223.54545410
TAACCGT1103.6379788E-1223.5454547
ACTAGTG400.00193032223.1250028
GTATCAA44050.023.0147551
CGCTCTC3400.022.8529429
GCCGCTC3450.022.52173827
CCTAGAC502.7004164E-422.22
ACGTATA1500.022.229
GTACCCT1107.4578566E-1121.8636361
TATAACT603.7231985E-521.5833324
GACGTAT1550.021.48387128
CCGCTCT3650.021.28767228
CGGGTAT705.0965045E-621.14285717
ACGGCGT705.0965045E-621.14285735
ACTGTGC1600.020.81258
GCAGCTT3650.020.78082319
TAGACCA450.003824110820.5555574
Produced by FastQC (version 0.11.5)