##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660563
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.421460178665775	33.0	33.0	33.0	27.0	33.0
2	31.629535714231647	33.0	33.0	33.0	27.0	33.0
3	31.35053431693873	33.0	33.0	33.0	27.0	33.0
4	31.676403310509368	33.0	33.0	33.0	27.0	33.0
5	31.778072644092994	33.0	33.0	33.0	27.0	33.0
6	34.762420844037585	37.0	37.0	37.0	27.0	37.0
7	34.96767454429025	37.0	37.0	37.0	27.0	37.0
8	35.12330693665858	37.0	37.0	37.0	33.0	37.0
9	35.26033550168568	37.0	37.0	37.0	33.0	37.0
10	35.23441670211623	37.0	37.0	37.0	33.0	37.0
11	35.210805025410146	37.0	37.0	37.0	33.0	37.0
12	35.19894847274219	37.0	37.0	37.0	33.0	37.0
13	35.21169668903647	37.0	37.0	37.0	33.0	37.0
14	35.22447215481339	37.0	37.0	37.0	33.0	37.0
15	35.25291758696748	37.0	37.0	37.0	33.0	37.0
16	35.24239474508866	37.0	37.0	37.0	33.0	37.0
17	35.23205810800786	37.0	37.0	37.0	33.0	37.0
18	35.20226231260304	37.0	37.0	37.0	33.0	37.0
19	35.167111993859784	37.0	37.0	37.0	33.0	37.0
20	35.16160154292626	37.0	37.0	37.0	33.0	37.0
21	35.13897993075604	37.0	37.0	37.0	33.0	37.0
22	35.026927030427075	37.0	37.0	37.0	27.0	37.0
23	35.0544732296541	37.0	37.0	37.0	27.0	37.0
24	35.071310382204274	37.0	37.0	37.0	33.0	37.0
25	35.07656650463317	37.0	37.0	37.0	33.0	37.0
26	34.96537347686746	37.0	37.0	37.0	27.0	37.0
27	34.97179073002878	37.0	37.0	37.0	27.0	37.0
28	34.979727898777256	37.0	37.0	37.0	27.0	37.0
29	34.98228480856481	37.0	37.0	37.0	27.0	37.0
30	34.972061710995014	37.0	37.0	37.0	27.0	37.0
31	34.958865392097344	37.0	37.0	37.0	27.0	37.0
32	34.93387913037818	37.0	37.0	37.0	27.0	37.0
33	34.915936859921004	37.0	37.0	37.0	27.0	37.0
34	34.86459277918987	37.0	37.0	37.0	27.0	37.0
35	34.778384499283185	37.0	37.0	37.0	27.0	37.0
36	34.78967486825632	37.0	37.0	37.0	27.0	37.0
37	34.77529773844433	37.0	37.0	37.0	27.0	37.0
38	34.73274918516478	37.0	37.0	37.0	27.0	37.0
39	34.598755909731544	37.0	37.0	37.0	27.0	37.0
40	34.35351964914777	37.0	37.0	37.0	27.0	37.0
41	34.51599620323875	37.0	37.0	37.0	27.0	37.0
42	34.51734808034964	37.0	37.0	37.0	27.0	37.0
43	34.081972499216576	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	18.0
16	38.0
17	32.0
18	30.0
19	46.0
20	79.0
21	217.0
22	569.0
23	1334.0
24	2392.0
25	4100.0
26	6475.0
27	9405.0
28	13195.0
29	17005.0
30	22068.0
31	27199.0
32	34450.0
33	44257.0
34	62100.0
35	116577.0
36	298977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.268354418882076	18.3709956506798	13.833956791403695	22.52669313903443
2	17.456926894179663	22.58119210431102	37.87420730498075	22.08767369652857
3	19.744823733693835	28.06878374961964	29.340729044769386	22.84566347191714
4	12.331753367960362	18.377808021339373	39.42591395521699	29.864524655483276
5	15.296345692992189	35.83503768754835	32.42400800529246	16.444608614167006
6	27.675482883540255	40.512714154440985	16.40433993426819	15.40746302775057
7	26.521315907793806	33.19077816953114	20.550045945655448	19.737859977019603
8	26.002818807592913	32.0008840943256	19.460369412152968	22.53592768592852
9	28.496903399070188	13.500150629084583	18.27986126985617	39.72308470198906
10	17.335363924409936	25.99630920896266	30.28159312586385	26.386733740763564
11	35.7740291236415	23.932917829185104	21.51225545481657	18.780797592356823
12	25.459040242944276	25.695505197838813	27.58449988873128	21.26095467048563
13	31.780617442999382	22.185923220041087	23.862674718384167	22.170784618575368
14	24.09929105929336	21.110931129960353	28.12328271489623	26.666495095850056
15	26.6695228161432	25.793906107365988	25.849767546774494	21.686803529716318
16	21.966262112773496	27.090527322904855	25.122963290405302	25.82024727391634
17	23.335700001362476	24.871208348030393	24.44354285662382	27.349548793983313
18	25.938782523392923	22.369705841834918	27.772824090964825	23.91868754380733
19	28.739726566580327	22.87245879651146	27.647627856843325	20.740186780064885
20	28.23288618950804	21.568268280239735	27.583591572643336	22.61525395760889
21	25.237411117486147	24.696660273130647	27.741638571945447	22.32429003743776
22	24.27792655658885	24.281862592969937	27.718930669746868	23.721280180694347
23	24.2979095105236	24.303510793065914	29.012524164992588	22.3860555314179
24	24.053723868881548	24.214647202462142	27.52031221851663	24.211316710139684
25	24.172259118358127	24.848349059817156	29.057031653301806	21.92236016852291
26	24.917835240544807	24.68530632203136	27.56451693479653	22.832341502627305
27	24.743287165645064	24.391314681567085	27.933141880486794	22.932256272301053
28	23.467859992158203	24.782041985397303	27.798559713456548	23.95153830898794
29	24.906935447489488	24.531195359110335	26.720085745038702	23.841783448361472
30	25.091323613341952	23.77244865364848	28.839187178210103	22.297040554799466
31	24.930248893746697	23.4940497726939	27.65080696315113	23.92489437040827
32	22.21544349289924	23.94941890478274	28.73215726584747	25.102980336470555
33	22.978883164815468	24.379052414379853	29.12515535989754	23.516909060907135
34	22.683831822248596	24.578125023654067	28.137664386288662	24.60037876780867
35	23.517363218951107	25.048632757208622	27.554979615873126	23.879024407967144
36	24.191939300263563	24.15333586652598	28.158101498267385	23.49662333494307
37	25.030315049435103	24.37299697379357	27.262199063526115	23.334488913245217
38	24.297758124508942	25.028347031244557	26.405354220566394	24.268540623680103
39	23.916265367572812	23.942000990064535	27.563760004723243	24.57797363763941
40	23.98272382800732	25.463127665340018	27.68305218427311	22.871096322379547
41	24.556476823558086	23.95517157333971	27.591917803449483	23.89643379965272
42	22.89562085675401	25.78164384017876	27.859416891348744	23.46331841171849
43	23.870244019117028	25.070280957304604	26.893876889865158	24.16559813371321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	38.5
2	71.0
3	339.0
4	607.0
5	607.0
6	849.0
7	1091.0
8	1120.5
9	1150.0
10	1574.0
11	1998.0
12	1998.0
13	3013.5
14	4029.0
15	7493.0
16	10957.0
17	11734.0
18	12511.0
19	12511.0
20	9777.5
21	7044.0
22	5259.0
23	3474.0
24	3509.0
25	3544.0
26	3544.0
27	4235.0
28	4926.0
29	5709.0
30	6492.0
31	8119.0
32	9746.0
33	9746.0
34	13864.5
35	17983.0
36	19297.0
37	20611.0
38	24708.0
39	28805.0
40	28805.0
41	33587.0
42	38369.0
43	42861.0
44	47353.0
45	50758.5
46	54164.0
47	54164.0
48	57523.0
49	60882.0
50	61931.0
51	62980.0
52	63984.5
53	64989.0
54	64989.0
55	59975.5
56	54962.0
57	48930.0
58	42898.0
59	38971.5
60	35045.0
61	35045.0
62	30173.5
63	25302.0
64	19011.0
65	12720.0
66	11147.5
67	9575.0
68	9575.0
69	7989.5
70	6404.0
71	5350.0
72	4296.0
73	3551.5
74	2807.0
75	2807.0
76	1962.5
77	1118.0
78	800.0
79	482.0
80	405.0
81	328.0
82	328.0
83	309.5
84	291.0
85	281.0
86	271.0
87	206.5
88	142.0
89	142.0
90	122.5
91	103.0
92	67.5
93	32.0
94	18.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	660563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.09941838192958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24442386360576	23.852110683056345
2	9.273998780322689	5.582839387248746
3	3.2804161984438087	2.962158588714574
4	1.6896031531904883	2.0342428882923187
5	1.093139623817227	1.6451433443569923
6	0.7572333510083826	1.3675370066851107
7	0.5621143254057431	1.1843519981204502
8	0.4273718993325494	1.029091648215224
9	0.31236269388675625	0.8461741869183668
>10	2.563773171728587	15.54941736596684
>50	0.41325934782467066	8.753793720440289
>100	0.33277438120712727	19.61598299423177
>500	0.03663097839644347	7.615971357664517
>1k	0.011866373283354928	5.585687534690679
>5k	0.0010318585463786891	2.3754972953977957
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8870	1.3427939500093102	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6427	0.9729579162017854	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3560	0.5389342121796105	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2754	0.41691708436591207	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	2327	0.3522752561072903	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2234	0.33819635674417126	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	2151	0.3256313175276242	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1870	0.28309184740895266	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1782	0.2697698781191196	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	1638	0.24797029200848367	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1475	0.22329437161936108	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1418	0.21466536878390102	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1297	0.1963476610103805	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	1275	0.1930171686879223	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1260	0.19074637846806436	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	1231	0.18635618404300575	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	1189	0.1799979714274036	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	1117	0.16909817837208563	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1117	0.16909817837208563	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1079	0.16334550981511226	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1077	0.16304273778579786	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	1041	0.15759284125813888	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1030	0.15592759509690976	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1029	0.15577620908225256	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1018	0.15411096292102344	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	978	0.14805552233473568	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	967	0.14639027617350656	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	960	0.1453305740709062	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	948	0.14351394189501984	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	945	0.14305978385104826	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.13579325514750296	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	893	0.13518771108887417	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	887	0.13427939500093103	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	880	0.13321969289833066	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	875	0.1324627628250447	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	859	0.13004058659052958	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	855	0.12943504253190083	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	852	0.12898088448792924	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	839	0.12701286629738573	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	837	0.1267100942680713	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	835	0.12640732223875695	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	824	0.1247420760775278	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	812	0.12292544390164148	No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG	805	0.12186574179904111	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	787	0.11914079353521163	No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC	786	0.11898940752055444	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	771	0.11671861730069652	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	770	0.11656723128603934	No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA	769	0.11641584527138216	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	751	0.11369089700755265	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	729	0.1103604046850944	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	729	0.1103604046850944	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	719	0.10884654453852244	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	707	0.10702991236263612	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	698	0.10566743823072137	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	694	0.1050618941720926	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	679	0.10279110395223469	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	663	0.10036892771771957	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	663	0.10036892771771957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.055440586287757E-4	0.0	0.0
2	0.0	0.0	6.055440586287757E-4	0.0	0.0
3	0.0	0.0	9.083160879431637E-4	0.0	0.0
4	0.0	0.0	0.0013624741319147454	1.5138601465719393E-4	0.0
5	0.0	0.0	0.0015138601465719394	3.0277202931438787E-4	0.0
6	0.0	0.0	0.0037846503664298483	3.0277202931438787E-4	0.0
7	0.0	0.0	0.007720686747516891	3.0277202931438787E-4	0.0
8	3.0277202931438787E-4	0.0	0.009688704938060412	3.0277202931438787E-4	0.0
9	3.0277202931438787E-4	0.0	0.011808109143261128	0.0012110881172575515	0.0
10	6.055440586287757E-4	0.0	0.014078899363119037	0.0012110881172575515	0.0
11	7.569300732859697E-4	0.0	0.02225374415460751	0.0012110881172575515	0.0
12	7.569300732859697E-4	0.0	0.024070376330493835	0.0013624741319147454	0.0
13	7.569300732859697E-4	0.0	0.025281464447751388	0.0013624741319147454	0.0
14	7.569300732859697E-4	0.0	0.02724948263829491	0.0013624741319147454	0.0
15	7.569300732859697E-4	0.0	0.02952027285815282	0.0015138601465719394	0.0
16	7.569300732859697E-4	0.0	0.03148829104869634	0.0015138601465719394	0.0
17	7.569300732859697E-4	0.0	0.032547993151296695	0.0015138601465719394	0.0
18	9.083160879431637E-4	0.0	0.03391046728321144	0.0015138601465719394	0.0
19	9.083160879431637E-4	0.0	0.034818783371154606	0.0015138601465719394	0.0
20	9.083160879431637E-4	0.0	0.03542432742978338	0.0018166321758863273	0.0
21	9.083160879431637E-4	0.0	0.037240959605669706	0.002270790219857909	0.0
22	9.083160879431637E-4	0.0	0.0375437316349841	0.002573562249172297	0.0
23	9.083160879431637E-4	0.0	0.03784650366429849	0.0031791063078010725	0.0
24	9.083160879431637E-4	0.0	0.037997889678955676	0.004541580439715818	0.0
25	9.083160879431637E-4	0.0	0.037997889678955676	0.005298510513001788	0.0
26	9.083160879431637E-4	0.0	0.03814927569361287	0.006358212615602145	0.0
27	9.083160879431637E-4	0.0	0.03814927569361287	0.007872072762174085	0.0
28	9.083160879431637E-4	0.0	0.03830066170827007	0.024827306403779805	0.0
29	0.0013624741319147454	0.0	0.03830066170827007	0.06343074014136427	0.0
30	0.0015138601465719394	0.0	0.03830066170827007	0.11459921309549581	0.0
31	0.0015138601465719394	0.0	0.03830066170827007	0.20346280369926864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTT	25	0.005494672	29.6	35
GGTATCA	1715	0.0	27.938776	1
GGACGTA	140	0.0	23.785713	27
CCGTGCA	110	3.6379788E-12	23.545454	10
TAACCGT	110	3.6379788E-12	23.545454	7
ACTAGTG	40	0.001930322	23.125002	8
GTATCAA	4405	0.0	23.014755	1
CGCTCTC	340	0.0	22.85294	29
GCCGCTC	345	0.0	22.521738	27
CCTAGAC	50	2.7004164E-4	22.2	2
ACGTATA	150	0.0	22.2	29
GTACCCT	110	7.4578566E-11	21.863636	1
TATAACT	60	3.7231985E-5	21.583332	4
GACGTAT	155	0.0	21.483871	28
CCGCTCT	365	0.0	21.287672	28
CGGGTAT	70	5.0965045E-6	21.142857	17
ACGGCGT	70	5.0965045E-6	21.142857	35
ACTGTGC	160	0.0	20.8125	8
GCAGCTT	365	0.0	20.780823	19
TAGACCA	45	0.0038241108	20.555557	4
>>END_MODULE