FastQCFastQC Report
Fri 10 Feb 2017
ERR1633151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799876
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63020.7878721201786277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42830.5354579959893784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24690.30867284429086506No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12760.15952472633258155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12220.15277367992038765No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10570.13214548254979522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.12927003685571264No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10040.12551945551560492No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8390.10489125814501248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT352.3874065E-531.71428710
GGTATCA10300.028.9174751
TCTCGCG405.9379065E-527.7520
AAACACG604.3193722E-827.74999815
TATCACG358.867027E-426.4285722
GACGTAT601.336557E-624.66666628
GTATCAA29500.023.7050861
ATACCGC400.001930676723.1256
CGCGAGG502.701106E-422.223
ACGTATA705.098722E-621.14285929
CGCTCTC2100.021.14285729
TGCTCGC2300.020.91304410
GCCGGCA2050.020.756115
GCGAACT2450.020.38775428
GTCGCTT555.1412144E-420.18181829
CGAACTA2400.020.04166629
ATACGGT656.898774E-519.92307733
ACACGAA759.259995E-619.73333417
GGACGTA759.259995E-619.73333427
TTCGCCG2350.019.6808524
Produced by FastQC (version 0.11.5)