##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799876
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847311333256656	33.0	33.0	33.0	27.0	33.0
2	31.95850606844061	33.0	33.0	33.0	33.0	33.0
3	31.679650345803598	33.0	33.0	33.0	27.0	33.0
4	31.95474173496892	33.0	33.0	33.0	33.0	33.0
5	32.0591591696713	33.0	33.0	33.0	33.0	33.0
6	35.20421290299996	37.0	37.0	37.0	33.0	37.0
7	35.35535633023119	37.0	37.0	37.0	33.0	37.0
8	35.47934679875381	37.0	37.0	37.0	33.0	37.0
9	35.59166545814601	37.0	37.0	37.0	33.0	37.0
10	35.59439713155539	37.0	37.0	37.0	33.0	37.0
11	35.60609644494897	37.0	37.0	37.0	33.0	37.0
12	35.60669653796339	37.0	37.0	37.0	33.0	37.0
13	35.60309097910176	37.0	37.0	37.0	33.0	37.0
14	35.57640809325446	37.0	37.0	37.0	33.0	37.0
15	35.60666653331266	37.0	37.0	37.0	33.0	37.0
16	35.59749386154854	37.0	37.0	37.0	33.0	37.0
17	35.57349514174697	37.0	37.0	37.0	33.0	37.0
18	35.55612369917337	37.0	37.0	37.0	33.0	37.0
19	35.54041876490856	37.0	37.0	37.0	33.0	37.0
20	35.54590336502158	37.0	37.0	37.0	33.0	37.0
21	35.5396836509659	37.0	37.0	37.0	33.0	37.0
22	35.449702203841596	37.0	37.0	37.0	33.0	37.0
23	35.49346398691797	37.0	37.0	37.0	33.0	37.0
24	35.49511799328896	37.0	37.0	37.0	33.0	37.0
25	35.522178437657836	37.0	37.0	37.0	33.0	37.0
26	35.40693432482035	37.0	37.0	37.0	33.0	37.0
27	35.41055488600733	37.0	37.0	37.0	33.0	37.0
28	35.42218543874301	37.0	37.0	37.0	33.0	37.0
29	35.423260605393835	37.0	37.0	37.0	33.0	37.0
30	35.436151353459785	37.0	37.0	37.0	33.0	37.0
31	35.41973255854658	37.0	37.0	37.0	33.0	37.0
32	35.409425961023956	37.0	37.0	37.0	33.0	37.0
33	35.4072381219089	37.0	37.0	37.0	33.0	37.0
34	35.37644834949417	37.0	37.0	37.0	33.0	37.0
35	35.319422010411614	37.0	37.0	37.0	33.0	37.0
36	35.33985767794008	37.0	37.0	37.0	33.0	37.0
37	35.33030119668549	37.0	37.0	37.0	33.0	37.0
38	35.319120713710625	37.0	37.0	37.0	33.0	37.0
39	35.20051983057374	37.0	37.0	37.0	33.0	37.0
40	34.98883201896294	37.0	37.0	37.0	27.0	37.0
41	35.15784821647355	37.0	37.0	37.0	33.0	37.0
42	35.18323465137096	37.0	37.0	37.0	33.0	37.0
43	34.84097285079187	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	18.0
16	42.0
17	38.0
18	38.0
19	30.0
20	48.0
21	151.0
22	395.0
23	941.0
24	1962.0
25	3437.0
26	5494.0
27	8473.0
28	11669.0
29	15443.0
30	20464.0
31	25803.0
32	33091.0
33	42887.0
34	61631.0
35	123045.0
36	444773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.596477454005374	19.665923218098804	13.97491611199736	23.762683215898463
2	16.140126719641547	21.88089153818842	39.30484225054883	22.6741394916212
3	18.06267471458076	27.51601498232226	30.278318139311594	24.142992163785387
4	12.213143037170761	17.941655956673284	40.09371452575149	29.751486480404466
5	13.787137006235966	36.83583455435592	34.75826253069226	14.61876590871585
6	28.37189764413484	40.50102765928719	17.220169126214564	13.906905570363406
7	26.301326705639372	32.92872895298771	22.67713995669329	18.092804384679624
8	24.784216553565802	34.99267386444899	20.347028789462367	19.876080792522842
9	26.905545359530723	15.112467432452032	19.73705924418285	38.2449279638344
10	15.579164770539434	27.52201591246643	32.933729728107856	23.965089588886276
11	33.81549140116718	24.32001960303847	23.426131050312797	18.43835794548155
12	23.539773664918062	25.94327120703709	29.668348594032075	20.848606534012774
13	28.92298306237467	22.4756087193515	25.979901884792145	22.62150633348169
14	22.80465972225695	21.41681960703909	28.191369662297657	27.587151008406302
15	24.490671054013372	27.864819046952277	25.23116082992864	22.413349069105713
16	22.190689556881317	28.52992213793138	25.45644574909111	23.822942556096194
17	22.477484010021552	26.96567968035045	26.17593226950177	24.380904040126218
18	24.02784931664408	25.78162115127845	27.858818116808102	22.331711415269368
19	25.89238832018963	25.281793678020094	28.121358810615643	20.70445919117463
20	25.475198655791647	25.05813401077167	27.782806334981924	21.683860998454758
21	23.44388380198931	26.340332751576494	27.89382354264911	22.32195990378509
22	22.918802414374227	26.12892498337242	28.068225574964117	22.88404702728923
23	22.704519200476074	26.04678725202406	28.844095834854404	22.40459771264546
24	22.806535012927004	26.32107976736394	27.827813311063217	23.04457190864584
25	23.109832023963715	26.392840890338004	28.50179277788057	21.995534307817714
26	23.12996014382229	26.457350889387854	28.05922417974786	22.35346478704199
27	23.147212817986787	26.4380979051753	27.563022268451608	22.8516670083863
28	22.615005325825503	26.455225559961793	28.16249018597883	22.767278928233875
29	23.585780796023386	26.213313063524847	27.42937655336577	22.771529587085997
30	23.40487775605219	26.252694167595976	28.27713295560812	22.065295120743716
31	23.371122523991218	25.887012486935472	27.909951042411574	22.831913946661732
32	22.005535858057996	25.98240227235221	28.4680375458196	23.544024323770184
33	22.208067250423817	26.18830918792413	28.781586145852607	22.82203741579945
34	22.73364871555091	25.84513099530427	28.2868844670924	23.134335822052417
35	22.719271487080498	26.79890383009366	27.92695367782006	22.554871005005776
36	23.281233591206636	26.115422890548036	27.983212397921676	22.62013112032365
37	23.59540728812966	25.735614020173124	27.75167650985903	22.917302181838185
38	23.448509518975442	26.154678975241165	27.389370352404622	23.007441153378775
39	22.773279858378046	25.99827973335867	28.022343463236805	23.20609694502648
40	22.802159334696878	26.68451109922038	28.14311218238827	22.370217383694474
41	22.88542224044727	25.63159789767414	28.220249138616488	23.262730723262106
42	22.48748556026184	26.665133095629823	27.956333231650905	22.891048112457433
43	22.906550515329876	26.07679190274493	27.947206817056642	23.069450764868556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	47.5
2	72.0
3	310.5
4	549.0
5	549.0
6	699.0
7	849.0
8	797.5
9	746.0
10	1035.5
11	1325.0
12	1325.0
13	2129.0
14	2933.0
15	5249.5
16	7566.0
17	8750.0
18	9934.0
19	9934.0
20	8928.5
21	7923.0
22	8144.5
23	8366.0
24	10226.0
25	12086.0
26	12086.0
27	14452.0
28	16818.0
29	19614.5
30	22411.0
31	25866.5
32	29322.0
33	29322.0
34	33516.5
35	37711.0
36	40420.5
37	43130.0
38	45742.5
39	48355.0
40	48355.0
41	51318.5
42	54282.0
43	54914.5
44	55547.0
45	57497.5
46	59448.0
47	59448.0
48	59417.0
49	59386.0
50	60155.5
51	60925.0
52	61098.5
53	61272.0
54	61272.0
55	56896.5
56	52521.0
57	46937.5
58	41354.0
59	37458.0
60	33562.0
61	33562.0
62	29655.0
63	25748.0
64	20592.0
65	15436.0
66	13235.0
67	11034.0
68	11034.0
69	9155.5
70	7277.0
71	6213.5
72	5150.0
73	4164.5
74	3179.0
75	3179.0
76	2372.0
77	1565.0
78	1214.0
79	863.0
80	665.0
81	467.0
82	467.0
83	372.5
84	278.0
85	254.0
86	230.0
87	185.0
88	140.0
89	140.0
90	104.0
91	68.0
92	46.0
93	24.0
94	12.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	799876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.176385739246776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.905911898478	43.451776988590126
2	8.419314307706824	8.617401533423278
3	2.2580767077024837	3.466806138665721
4	1.0254197493966288	2.0990910655905455
5	0.6021905078123575	1.5408966858159057
6	0.3883439238424215	1.1924423067631462
7	0.3089871517181114	1.1068991965358064
8	0.23086463451922162	0.945185407176473
9	0.1771533893081159	0.8159463167622385
>10	1.358524546266661	14.019718232888682
>50	0.19621953278395388	6.993334779601175
>100	0.12130364437990492	11.604423201299301
>500	0.00570548838590222	1.7788311642790242
>1k	0.0017364529870137193	1.5672045905669822
>5k	2.480647124305313E-4	0.8000423920415651
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6302	0.7878721201786277	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4283	0.5354579959893784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2469	0.30867284429086506	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1276	0.15952472633258155	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1222	0.15277367992038765	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1057	0.13214548254979522	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.12927003685571264	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1004	0.12551945551560492	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	839	0.10489125814501248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.2501937800359056E-4	0.0	0.0
2	0.0	0.0	2.500387560071811E-4	0.0	0.0
3	0.0	0.0	3.7505813401077166E-4	0.0	0.0
4	0.0	0.0	5.000775120143623E-4	0.0	0.0
5	0.0	0.0	0.0010001550240287245	0.0	0.0
6	0.0	0.0	0.0017502712920502677	0.0	0.0
7	1.2501937800359056E-4	0.0	0.004375678230125669	0.0	0.0
8	1.2501937800359056E-4	0.0	0.005250813876150803	0.0	0.0
9	1.2501937800359056E-4	0.0	0.006375988278183118	8.751356460251339E-4	0.0
10	5.000775120143623E-4	0.0	0.006876065790197481	0.001125174402032315	0.0
11	5.000775120143623E-4	0.0	0.009751511484280063	0.0012501937800359056	0.0
12	5.000775120143623E-4	0.0	0.010501627752301606	0.0012501937800359056	0.0
13	5.000775120143623E-4	0.0	0.01125174402032315	0.0012501937800359056	0.0
14	5.000775120143623E-4	0.0	0.011751821532337512	0.0012501937800359056	0.0
15	5.000775120143623E-4	0.0	0.01337707344638419	0.0012501937800359056	0.0
16	5.000775120143623E-4	0.0	0.014752286604423686	0.0012501937800359056	0.0
17	6.250968900179528E-4	0.0	0.015002325360430866	0.0012501937800359056	0.0
18	6.250968900179528E-4	0.0	0.015127344738434457	0.0012501937800359056	0.0
19	6.250968900179528E-4	0.0	0.015252364116438048	0.0012501937800359056	0.0
20	6.250968900179528E-4	0.0	0.01562742225044882	0.0013752131580394961	0.0
21	6.250968900179528E-4	0.0	0.016127499762463183	0.0017502712920502677	0.0
22	6.250968900179528E-4	0.0	0.016502557896473955	0.0021253294260610393	0.0
23	6.250968900179528E-4	0.0	0.016502557896473955	0.003125484450089764	0.0
24	6.250968900179528E-4	0.0	0.016502557896473955	0.004000620096114898	0.0
25	6.250968900179528E-4	0.0	0.016627577274477542	0.004125639474118489	0.0
26	6.250968900179528E-4	0.0	0.016627577274477542	0.0055008526321579845	0.0
27	7.501162680215433E-4	0.0	0.016627577274477542	0.00675104641219389	0.0
28	7.501162680215433E-4	0.0	0.016627577274477542	0.014002170336402142	0.0
29	7.501162680215433E-4	0.0	0.016627577274477542	0.03450534832899099	0.0
30	7.501162680215433E-4	0.0	0.016627577274477542	0.06125949522175937	0.0
31	8.751356460251339E-4	0.0	0.016627577274477542	0.12639459116163004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGCT	35	2.3874065E-5	31.714287	10
GGTATCA	1030	0.0	28.917475	1
TCTCGCG	40	5.9379065E-5	27.75	20
AAACACG	60	4.3193722E-8	27.749998	15
TATCACG	35	8.867027E-4	26.428572	2
GACGTAT	60	1.336557E-6	24.666666	28
GTATCAA	2950	0.0	23.705086	1
ATACCGC	40	0.0019306767	23.125	6
CGCGAGG	50	2.701106E-4	22.2	23
ACGTATA	70	5.098722E-6	21.142859	29
CGCTCTC	210	0.0	21.142857	29
TGCTCGC	230	0.0	20.913044	10
GCCGGCA	205	0.0	20.7561	15
GCGAACT	245	0.0	20.387754	28
GTCGCTT	55	5.1412144E-4	20.181818	29
CGAACTA	240	0.0	20.041666	29
ATACGGT	65	6.898774E-5	19.923077	33
ACACGAA	75	9.259995E-6	19.733334	17
GGACGTA	75	9.259995E-6	19.733334	27
TTCGCCG	235	0.0	19.68085	24
>>END_MODULE