FastQCFastQC Report
Fri 10 Feb 2017
ERR1633150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853764
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57390.672199811657554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.47706391930322667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22650.2652957960279422No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA12760.1494558215150791No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12660.14828453764740607No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11890.1392656518663237No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG11630.13622031381037383No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG11530.1350490299427008No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11140.13048102285877597No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10780.12626440093515304No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10390.12169639385122821No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG10140.11876818418204563No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9660.11314602161721507No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9600.11244325129661124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.11220899452307664No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8660.10143318294048471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC358.867518E-426.4285724
GGTATCA12000.025.91
TTGCGGT809.786163E-1025.43750235
CGGGCGA601.3367335E-624.66666817
GTATCAA32950.023.35661
ATCCGTA802.7237547E-823.12500212
CATCCGT802.7237547E-823.12500211
CTCGCAT753.7384416E-722.222
TACTCGC753.7384416E-722.220
ACATCCG957.1377144E-921.42105310
CCGCTCT1500.020.96666728
CTGTTCG450.003825014720.5555559
TGCGGTA1001.2885721E-820.34999836
CGCTCTC1550.020.29032329
TCGCATC851.2445398E-619.58823623
TTACTCG851.2445398E-619.58823619
GCCGCTC1700.019.58823627
GACGTGG500.00703343718.4999986
TGCGGTT500.00703343718.49999836
GCCGGCA1850.018.015
Produced by FastQC (version 0.11.5)