##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853764
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739936329008952	33.0	33.0	33.0	27.0	33.0
2	31.86508098256661	33.0	33.0	33.0	33.0	33.0
3	31.59967977099058	33.0	33.0	33.0	27.0	33.0
4	31.881905304041865	33.0	33.0	33.0	33.0	33.0
5	31.990507915536377	33.0	33.0	33.0	33.0	33.0
6	35.12887636396007	37.0	37.0	37.0	33.0	37.0
7	35.28233563373485	37.0	37.0	37.0	33.0	37.0
8	35.42126746969889	37.0	37.0	37.0	33.0	37.0
9	35.54904634067494	37.0	37.0	37.0	33.0	37.0
10	35.540664633317874	37.0	37.0	37.0	33.0	37.0
11	35.557186763555265	37.0	37.0	37.0	33.0	37.0
12	35.528698797325724	37.0	37.0	37.0	33.0	37.0
13	35.54014458328063	37.0	37.0	37.0	33.0	37.0
14	35.529127487221295	37.0	37.0	37.0	33.0	37.0
15	35.55429603496985	37.0	37.0	37.0	33.0	37.0
16	35.541192882342195	37.0	37.0	37.0	33.0	37.0
17	35.51357752259407	37.0	37.0	37.0	33.0	37.0
18	35.50126147272548	37.0	37.0	37.0	33.0	37.0
19	35.48809975590444	37.0	37.0	37.0	33.0	37.0
20	35.49328034445116	37.0	37.0	37.0	33.0	37.0
21	35.47784282307523	37.0	37.0	37.0	33.0	37.0
22	35.38498929446545	37.0	37.0	37.0	33.0	37.0
23	35.435759764993605	37.0	37.0	37.0	33.0	37.0
24	35.431905069785095	37.0	37.0	37.0	33.0	37.0
25	35.44764712496662	37.0	37.0	37.0	33.0	37.0
26	35.34488102098472	37.0	37.0	37.0	33.0	37.0
27	35.33793882150103	37.0	37.0	37.0	33.0	37.0
28	35.37347088891075	37.0	37.0	37.0	33.0	37.0
29	35.373212035175996	37.0	37.0	37.0	33.0	37.0
30	35.36949438018	37.0	37.0	37.0	33.0	37.0
31	35.35178573938465	37.0	37.0	37.0	33.0	37.0
32	35.34606167512334	37.0	37.0	37.0	33.0	37.0
33	35.33826326713237	37.0	37.0	37.0	33.0	37.0
34	35.31816169339537	37.0	37.0	37.0	33.0	37.0
35	35.23983794116407	37.0	37.0	37.0	33.0	37.0
36	35.27108779475358	37.0	37.0	37.0	33.0	37.0
37	35.26407063310236	37.0	37.0	37.0	33.0	37.0
38	35.24718657614985	37.0	37.0	37.0	33.0	37.0
39	35.14615397229211	37.0	37.0	37.0	33.0	37.0
40	34.91815771103021	37.0	37.0	37.0	27.0	37.0
41	35.10000538790579	37.0	37.0	37.0	27.0	37.0
42	35.127974475381954	37.0	37.0	37.0	33.0	37.0
43	34.77191589244803	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	25.0
16	43.0
17	34.0
18	28.0
19	32.0
20	69.0
21	167.0
22	415.0
23	1120.0
24	2218.0
25	3840.0
26	6208.0
27	9365.0
28	13108.0
29	17377.0
30	22612.0
31	28961.0
32	36332.0
33	47870.0
34	68385.0
35	135873.0
36	459678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.984009632638525	20.245758781115157	13.502326169761197	23.267905416485117
2	16.52013905482077	21.934047347979067	39.13751341120029	22.408300185999877
3	18.16427021987341	27.505610449726152	29.835528319301353	24.494591011099086
4	11.989964439821778	18.113202243242863	40.079225640809405	29.817607676125956
5	14.057280466264682	36.722794589605556	34.87017489610712	14.349750048022639
6	28.909042779971983	40.66861568302247	16.969443546460145	13.452897990545395
7	27.258118168486845	32.20679250940541	22.755468724378165	17.779620597729583
8	24.813531608266455	35.05195815236998	20.297178142906002	19.83733209645757
9	26.49303554612282	14.933635056057645	20.122539718235952	38.450789679583586
10	15.618953247033138	27.571553731476143	33.354065057791146	23.45542796369957
11	34.17208971097399	24.21769950478118	23.33197464404683	18.278236140197993
12	23.692027304969525	25.87389489367085	29.729878514437246	20.704199286922382
13	28.34577236800802	22.76870423208287	26.041857000295167	22.843666399613944
14	22.6270960124812	21.298625849766445	27.90314419441438	28.171133943337974
15	24.193336800333583	28.436546867752682	25.554368654569647	21.815747677344092
16	23.086824930542868	28.446151395467602	25.099324871978673	23.36769880201086
17	22.96981367216233	26.850042868989554	25.768596473967044	24.41154698488107
18	23.39897208127773	26.07547284729738	28.179801443958752	22.345753627466138
19	26.109322951073132	25.620194807932872	27.5269278161178	20.743554424876194
20	25.379964486673135	25.52215834820864	27.701331984014317	21.396545181103914
21	23.140235474908756	26.874288445050386	27.66607633959736	22.319399740443497
22	23.32986633308502	26.185924916018948	27.774888610904185	22.70932013999185
23	22.619599795728092	26.36466283422585	28.815925712491975	22.19981165755408
24	22.890634882707634	27.152351235235965	26.890217905650744	23.066795976405658
25	23.05391185386125	26.989659906016183	28.16375485497163	21.79267338515093
26	23.745672106108948	26.627733190905218	27.43334223509073	22.193252467895107
27	23.43071387409167	26.465978888779567	26.957098214494874	23.14620902263389
28	22.641737060827115	26.449346657858612	28.24761877989702	22.661297501417256
29	24.20610379449122	26.628553089612588	27.235512389840753	21.929830726055442
30	23.88107252121195	26.00156483524721	27.689619145337584	22.427743498203252
31	23.128874021392328	25.89509513167573	27.955500583299365	23.020530263632573
32	21.948454139551448	26.17456346250252	28.160709516915684	23.716272881030356
33	22.36262011516063	25.97977895530849	28.71296986052352	22.94463106900736
34	23.068084388660097	26.13274862842659	28.290019255906785	22.509147727006525
35	23.16740926063877	26.932852638434042	27.036042747176037	22.863695353751154
36	23.38948468194958	26.499126222234715	27.14485501848286	22.966534077332845
37	23.309720250561046	26.030261290005203	27.68985340211112	22.97016505732263
38	23.131685102674744	26.06422852216772	27.209041374431344	23.595045000726195
39	22.73532264185419	26.109322951073132	27.948121494933027	23.207232912139656
40	23.173499936750673	27.077623324478427	27.542974405104925	22.20590233366598
41	23.18685257284214	25.8156820854475	27.753219859352235	23.24424548235812
42	22.212578651711716	27.635037317104022	27.43966716797616	22.712716863208097
43	23.668133114068993	25.69363430643597	27.123537652091212	23.514694927403827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	48.0
2	66.0
3	294.0
4	522.0
5	522.0
6	656.0
7	790.0
8	780.0
9	770.0
10	989.0
11	1208.0
12	1208.0
13	1950.5
14	2693.0
15	4968.5
16	7244.0
17	8152.0
18	9060.0
19	9060.0
20	8059.5
21	7059.0
22	7279.0
23	7499.0
24	9334.0
25	11169.0
26	11169.0
27	13515.0
28	15861.0
29	18784.0
30	21707.0
31	25343.5
32	28980.0
33	28980.0
34	36995.5
35	45011.0
36	48348.5
37	51686.0
38	54187.0
39	56688.0
40	56688.0
41	58414.0
42	60140.0
43	62694.0
44	65248.0
45	64276.0
46	63304.0
47	63304.0
48	61912.5
49	60521.0
50	62005.5
51	63490.0
52	63553.0
53	63616.0
54	63616.0
55	61565.5
56	59515.0
57	50414.5
58	41314.0
59	37887.5
60	34461.0
61	34461.0
62	30707.5
63	26954.0
64	20687.0
65	14420.0
66	12337.0
67	10254.0
68	10254.0
69	8772.0
70	7290.0
71	7053.0
72	6816.0
73	5966.5
74	5117.0
75	5117.0
76	3250.0
77	1383.0
78	1037.5
79	692.0
80	560.5
81	429.0
82	429.0
83	359.5
84	290.0
85	265.5
86	241.0
87	180.5
88	120.0
89	120.0
90	99.5
91	79.0
92	51.5
93	24.0
94	13.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	853764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.19113273927265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.22013854787185	42.41266280106843
2	7.776835204484553	7.651026656704965
3	1.967488693205045	2.9034899241140235
4	0.8721904567192684	1.716161461216175
5	0.5538444023029768	1.362211675529443
6	0.3580963413381539	1.056909879612781
7	0.25917513188207064	0.8924382820590604
8	0.21610251655977553	0.8504262061906229
9	0.1606873727090467	0.7113954492410125
>10	1.2660444780543116	12.72299078587506
>50	0.1742139866480713	5.997654047554167
>100	0.15919107112560388	14.885769905843771
>500	0.013084197626647738	4.1706997345106975
>1k	0.002665299516539354	1.9821312467110452
>5k	2.422999560490322E-4	0.6840319437687726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5739	0.672199811657554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4073	0.47706391930322667	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.2652957960279422	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1276	0.1494558215150791	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1266	0.14828453764740607	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1189	0.1392656518663237	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1163	0.13622031381037383	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1153	0.1350490299427008	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1114	0.13048102285877597	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1078	0.12626440093515304	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1039	0.12169639385122821	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1014	0.11876818418204563	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	966	0.11314602161721507	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	960	0.11244325129661124	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.11220899452307664	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	866	0.10143318294048471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.513851603019101E-4	0.0	0.0
2	0.0	0.0	4.685135470692135E-4	0.0	0.0
3	0.0	0.0	5.856419338365169E-4	0.0	0.0
4	0.0	0.0	8.198987073711236E-4	0.0	0.0
5	0.0	0.0	0.0010541554809057304	0.0	0.0
6	0.0	0.0	0.0021083109618114607	0.0	0.0
7	0.0	0.0	0.0038652367633210116	2.3425677353460674E-4	0.0
8	0.0	0.0	0.004333750310390225	2.3425677353460674E-4	0.0
9	0.0	0.0	0.0051536490177613485	5.856419338365169E-4	0.0
10	3.513851603019101E-4	0.0	0.005973547725132472	5.856419338365169E-4	0.0
11	3.513851603019101E-4	0.0	0.009136014167849663	8.198987073711236E-4	0.0
12	3.513851603019101E-4	0.0	0.009487399328151574	8.198987073711236E-4	0.0
13	3.513851603019101E-4	0.0	0.01042442642229	8.198987073711236E-4	0.0
14	3.513851603019101E-4	0.0	0.011361453516428428	8.198987073711236E-4	0.0
15	3.513851603019101E-4	0.0	0.012181352223799551	9.37027094138427E-4	0.0
16	4.685135470692135E-4	0.0	0.013001250931170674	9.37027094138427E-4	0.0
17	4.685135470692135E-4	0.0	0.013586892865007192	0.001522669027974944	0.0
18	4.685135470692135E-4	0.0	0.013821149638541799	0.0017569258015095507	0.0
19	4.685135470692135E-4	0.0	0.014289663185611012	0.0017569258015095507	0.0
20	7.027703206038202E-4	0.0	0.014641048345912922	0.0021083109618114607	0.0
21	7.027703206038202E-4	0.0	0.014992433506214832	0.002459696122113371	1.1712838676730337E-4
22	0.0010541554809057304	0.0	0.01557807544005135	0.003045338055949888	1.1712838676730337E-4
23	0.0010541554809057304	0.0	0.01557807544005135	0.003396723216251798	1.1712838676730337E-4
24	0.0010541554809057304	0.0	0.01557807544005135	0.004099493536855618	1.1712838676730337E-4
25	0.0010541554809057304	0.0	0.01557807544005135	0.004802263857459439	1.1712838676730337E-4
26	0.0011712838676730339	0.0	0.01557807544005135	0.006090676111899776	1.1712838676730337E-4
27	0.0012884122544403371	0.0	0.01557807544005135	0.007027703206038203	1.1712838676730337E-4
28	0.0012884122544403371	0.0	0.015695203826818653	0.01557807544005135	1.1712838676730337E-4
29	0.0012884122544403371	0.0	0.015695203826818653	0.03513851603019101	1.1712838676730337E-4
30	0.0012884122544403371	0.0	0.015695203826818653	0.061375274666066966	1.1712838676730337E-4
31	0.0012884122544403371	0.0	0.015695203826818653	0.1252102454542473	1.1712838676730337E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCC	35	8.867518E-4	26.428572	4
GGTATCA	1200	0.0	25.9	1
TTGCGGT	80	9.786163E-10	25.437502	35
CGGGCGA	60	1.3367335E-6	24.666668	17
GTATCAA	3295	0.0	23.3566	1
ATCCGTA	80	2.7237547E-8	23.125002	12
CATCCGT	80	2.7237547E-8	23.125002	11
CTCGCAT	75	3.7384416E-7	22.2	22
TACTCGC	75	3.7384416E-7	22.2	20
ACATCCG	95	7.1377144E-9	21.421053	10
CCGCTCT	150	0.0	20.966667	28
CTGTTCG	45	0.0038250147	20.555555	9
TGCGGTA	100	1.2885721E-8	20.349998	36
CGCTCTC	155	0.0	20.290323	29
TCGCATC	85	1.2445398E-6	19.588236	23
TTACTCG	85	1.2445398E-6	19.588236	19
GCCGCTC	170	0.0	19.588236	27
GACGTGG	50	0.007033437	18.499998	6
TGCGGTT	50	0.007033437	18.499998	36
GCCGGCA	185	0.0	18.0	15
>>END_MODULE