FastQCFastQC Report
Fri 10 Feb 2017
ERR1633149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004532
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57150.5689216470953639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.3816702703348425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21980.21880836051016792No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10610.10562132415891182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.10183846806273966No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10190.10144027268419523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.027.292081
GCGTCTC851.9754225E-923.9411779
GTATCAA29300.023.3617741
GCGTACT400.001931018523.12517
CGAGCGT957.1413524E-921.4210536
GCTACGA1059.822543E-1021.1428572
GCTACCG656.901025E-519.9230777
ACGAGCG1052.2586391E-819.380955
TTAACGG1950.018.9743635
GGCTACG1205.171387E-918.51
TACCGGC500.007034281318.4999987
TAGGACC2350.018.1063844
GCCGGCA1451.546141E-1017.8620715
CCGTCTA1258.574716E-917.7626
AGGACCG1156.402479E-817.6956525
AGGTTAC1502.5102054E-1017.2666667
CGCCGTT752.0669396E-417.26666625
AACGGCC2150.017.20930337
TTCGCCG1401.8681021E-917.17857224
CGTCTCC1452.9776857E-916.58620810
Produced by FastQC (version 0.11.5)