##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004532
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90737477750833	33.0	33.0	33.0	33.0	33.0
2	32.01895907746095	33.0	33.0	33.0	33.0	33.0
3	31.742013196194844	33.0	33.0	33.0	27.0	33.0
4	32.020539913113765	33.0	33.0	33.0	33.0	33.0
5	32.127093014458474	33.0	33.0	33.0	33.0	33.0
6	35.29046660534458	37.0	37.0	37.0	33.0	37.0
7	35.44322928488092	37.0	37.0	37.0	33.0	37.0
8	35.58032297627154	37.0	37.0	37.0	33.0	37.0
9	35.68660630024728	37.0	37.0	37.0	33.0	37.0
10	35.68790043522755	37.0	37.0	37.0	33.0	37.0
11	35.70606312193141	37.0	37.0	37.0	33.0	37.0
12	35.693369648751855	37.0	37.0	37.0	33.0	37.0
13	35.6991982336053	37.0	37.0	37.0	33.0	37.0
14	35.67765885009138	37.0	37.0	37.0	33.0	37.0
15	35.69431237631056	37.0	37.0	37.0	33.0	37.0
16	35.68860325007068	37.0	37.0	37.0	33.0	37.0
17	35.67588289870308	37.0	37.0	37.0	33.0	37.0
18	35.66388626743598	37.0	37.0	37.0	33.0	37.0
19	35.658025826952255	37.0	37.0	37.0	33.0	37.0
20	35.66034830149761	37.0	37.0	37.0	33.0	37.0
21	35.65106835820063	37.0	37.0	37.0	33.0	37.0
22	35.56778579477807	37.0	37.0	37.0	33.0	37.0
23	35.613302513011035	37.0	37.0	37.0	33.0	37.0
24	35.607208132742414	37.0	37.0	37.0	33.0	37.0
25	35.63488868448193	37.0	37.0	37.0	33.0	37.0
26	35.51576953247881	37.0	37.0	37.0	33.0	37.0
27	35.53474055580111	37.0	37.0	37.0	33.0	37.0
28	35.55747651642755	37.0	37.0	37.0	33.0	37.0
29	35.55505051108377	37.0	37.0	37.0	33.0	37.0
30	35.55378126331466	37.0	37.0	37.0	33.0	37.0
31	35.54615283535019	37.0	37.0	37.0	33.0	37.0
32	35.54014207611107	37.0	37.0	37.0	33.0	37.0
33	35.534016835700605	37.0	37.0	37.0	33.0	37.0
34	35.52283750044797	37.0	37.0	37.0	33.0	37.0
35	35.46102861830185	37.0	37.0	37.0	33.0	37.0
36	35.483210091863675	37.0	37.0	37.0	33.0	37.0
37	35.48244953869065	37.0	37.0	37.0	33.0	37.0
38	35.4822315267209	37.0	37.0	37.0	33.0	37.0
39	35.371730318197926	37.0	37.0	37.0	33.0	37.0
40	35.15827569455229	37.0	37.0	37.0	33.0	37.0
41	35.33316907773968	37.0	37.0	37.0	33.0	37.0
42	35.366208343785964	37.0	37.0	37.0	33.0	37.0
43	35.031441507089866	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	22.0
16	52.0
17	57.0
18	36.0
19	34.0
20	60.0
21	145.0
22	361.0
23	958.0
24	2081.0
25	3640.0
26	5980.0
27	9192.0
28	12795.0
29	17626.0
30	23434.0
31	30050.0
32	38775.0
33	51470.0
34	74950.0
35	154318.0
36	578490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67692019766419	20.409105931916553	14.015481836317809	23.89849203410145
2	15.913181461615958	21.705928730991147	39.50048380738493	22.880406000007962
3	17.502478766231437	27.578613722609134	30.47797382263581	24.44093368852361
4	11.958205413067976	18.084142665440226	40.6731691971983	29.284482724293504
5	13.481501833689718	37.039039074912495	35.51325393317485	13.966205158222934
6	28.459720546483336	40.78217518207484	17.39397052557808	13.364133745863747
7	26.267356341062303	32.69403065308024	23.467146890293193	17.571466115564263
8	24.524952913396486	35.35775863785325	20.71422314072623	19.403065308024033
9	26.413294947298844	15.344259814520592	20.498898989778326	37.74354624840225
10	15.092998530659052	28.097661398541806	33.67807098230818	23.131269088490956
11	33.284952594840185	24.232080212477054	24.32018093998001	18.16278625270275
12	23.173378249772032	26.28537468194144	30.168476464662152	20.372770603624375
13	28.117172972090486	22.61620336634373	26.14700178789725	23.11962187366853
14	22.081526521803188	21.463427745457587	28.549115408966564	27.905930323772658
15	23.820147093372835	28.804159548924275	25.159278151417773	22.216415206285117
16	22.25912166063401	28.825662099365672	25.634623884555197	23.280592355445123
17	22.196007693134714	27.59095777934401	26.670529161838548	23.542505365682725
18	23.152572541243085	26.842151369991203	28.275953379285077	21.729322709480634
19	24.894677322374996	26.2050387643201	28.063615693676258	20.836668219628642
20	24.689706251269246	26.04994166437704	27.94515256855929	21.31519951579442
21	23.03898730951329	26.998044860691344	28.02349750928791	21.93947032050746
22	22.39799229890138	26.940007884268496	28.329311560010034	22.33268825682009
23	22.35060704885459	26.672321041041997	28.797390227489018	22.17968168261439
24	22.384553204875505	27.145476699597427	27.696380005813655	22.77359008971342
25	22.760648739910724	26.932442172076147	28.432643260742317	21.874265827270808
26	22.83929232717325	27.03885988699215	28.133598531455444	21.988249254379152
27	22.597089988173597	27.192861949644215	27.697275945415377	22.512772116766815
28	22.23572768214452	26.8589751247347	28.45205528544636	22.45324190767442
29	23.236591766115964	26.888839778125533	27.809567042164907	22.065001413593592
30	22.996579501698303	26.77017755531929	28.189644530985575	22.043598411996832
31	22.80325564541498	26.716918923438975	28.09636726356154	22.383458167584507
32	21.832953056746824	26.676302994827445	28.48550369724409	23.005240251181643
33	21.738978947410338	26.86036880855961	28.967618751816765	22.43303349221329
34	22.703607251934237	26.281691374689903	28.407656500738653	22.607044872637207
35	22.426761915001215	27.19037322852831	28.009461122194217	22.37340373427626
36	23.128780367375057	26.871617828003487	27.786670807898606	22.21293099672285
37	22.949194251651512	26.366706088009146	27.88303408950636	22.801065570832986
38	22.95009019125324	26.772566727590558	27.79294238511068	22.484400696045522
39	22.270171582388613	26.68257457203952	28.315275172916344	22.731978672655526
40	22.326416679608016	27.124571442223843	28.408154244961832	22.14085763320631
41	22.459513484886497	26.260188824248505	28.26978135091764	23.01051633994736
42	21.909904313650536	27.322275447671153	28.172223483174253	22.595596755504054
43	22.643181103240114	26.405032393194045	28.188947689073117	22.76283881449272
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	59.5
2	89.0
3	305.0
4	521.0
5	521.0
6	685.5
7	850.0
8	761.0
9	672.0
10	976.5
11	1281.0
12	1281.0
13	1995.5
14	2710.0
15	4910.5
16	7111.0
17	8343.0
18	9575.0
19	9575.0
20	9014.5
21	8454.0
22	9442.0
23	10430.0
24	13426.5
25	16423.0
26	16423.0
27	20165.0
28	23907.0
29	28211.0
30	32515.0
31	37579.5
32	42644.0
33	42644.0
34	48941.5
35	55239.0
36	59250.5
37	63262.0
38	66191.0
39	69120.0
40	69120.0
41	72127.0
42	75134.0
43	76215.5
44	77297.0
45	76257.0
46	75217.0
47	75217.0
48	73281.0
49	71345.0
50	71255.0
51	71165.0
52	69152.0
53	67139.0
54	67139.0
55	63682.0
56	60225.0
57	52433.5
58	44642.0
59	41054.0
60	37466.0
61	37466.0
62	32937.0
63	28408.0
64	23231.0
65	18054.0
66	15287.5
67	12521.0
68	12521.0
69	10359.0
70	8197.0
71	6982.5
72	5768.0
73	4439.5
74	3111.0
75	3111.0
76	2347.5
77	1584.0
78	1207.0
79	830.0
80	657.5
81	485.0
82	485.0
83	439.0
84	393.0
85	373.0
86	353.0
87	273.5
88	194.0
89	194.0
90	161.0
91	128.0
92	81.0
93	34.0
94	21.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1004532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.28819265755445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81483227843123	48.86650004821613
2	7.848898995548784	8.83600677622271
3	1.7897444672245693	3.0222444413678615
4	0.7496253876177839	1.68780232956895
5	0.47719567616323316	1.3430241077614016
6	0.318652129812634	1.0761811484180155
7	0.2396657322325945	0.9443245636525495
8	0.18281469866299382	0.8232247183180287
9	0.14744950233172377	0.7469699395057733
>10	1.172325432951968	13.300711323642403
>50	0.15424181296261585	6.028346291632712
>100	0.0980977285156293	9.878369392090292
>500	0.005380131286855646	1.9472474809357123
>1k	8.966885478092743E-4	0.9221413031802868
>5k	1.7933770956185484E-4	0.5769061354871745
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5715	0.5689216470953639	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3834	0.3816702703348425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2198	0.21880836051016792	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1061	0.10562132415891182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.10183846806273966	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1019	0.10144027268419523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.954884463610916E-5	0.0	0.0
2	0.0	0.0	1.9909768927221832E-4	0.0	0.0
3	0.0	0.0	1.9909768927221832E-4	9.954884463610916E-5	0.0
4	0.0	0.0	1.9909768927221832E-4	9.954884463610916E-5	0.0
5	0.0	0.0	2.9864653390832745E-4	9.954884463610916E-5	0.0
6	0.0	0.0	0.0011945861356333098	9.954884463610916E-5	0.0
7	0.0	0.0	0.002588269960538838	1.9909768927221832E-4	0.0
8	0.0	0.0	0.0036833072515360388	2.9864653390832745E-4	0.0
9	9.954884463610916E-5	0.0	0.004579246853261021	6.968419124527641E-4	0.0
10	4.977442231805458E-4	0.0	0.005375637610349895	7.963907570888733E-4	0.0
11	4.977442231805458E-4	0.0	0.007267065658435969	7.963907570888733E-4	0.0
12	4.977442231805458E-4	0.0	0.007963907570888733	8.959396017249823E-4	0.0
13	4.977442231805458E-4	0.0	0.008760298327977605	8.959396017249823E-4	0.0
14	4.977442231805458E-4	0.0	0.009258042551158151	8.959396017249823E-4	0.0
15	4.977442231805458E-4	0.0	0.01055217753142757	0.0010950372909972006	0.0
16	4.977442231805458E-4	0.0	0.01174676366706088	0.0010950372909972006	0.0
17	4.977442231805458E-4	0.0	0.011945861356333099	0.001294134980269419	0.0
18	4.977442231805458E-4	0.0	0.012642703268785863	0.0014932326695416373	0.0
19	4.977442231805458E-4	0.0	0.012742252113421972	0.0014932326695416373	0.0
20	4.977442231805458E-4	0.0	0.0130408986473303	0.0016923303588138555	0.0
21	4.977442231805458E-4	0.0	0.013538642870510844	0.0022896234266305106	0.0
22	4.977442231805458E-4	0.0	0.01403638709369139	0.003185563028355493	0.0
23	4.977442231805458E-4	0.0	0.01403638709369139	0.003882404940808257	0.0
24	4.977442231805458E-4	0.0	0.01403638709369139	0.004479698008624912	0.0
25	4.977442231805458E-4	0.0	0.01403638709369139	0.004778344542533239	0.0
26	4.977442231805458E-4	0.0	0.01403638709369139	0.005176539921077676	0.0
27	4.977442231805458E-4	0.0	0.01403638709369139	0.006172028367438767	0.0
28	4.977442231805458E-4	0.0	0.01403638709369139	0.013339545181238626	0.0
29	4.977442231805458E-4	0.0	0.01403638709369139	0.027873676498110563	0.0
30	4.977442231805458E-4	0.0	0.01403638709369139	0.05554825530694891	0.0
31	4.977442231805458E-4	0.0	0.01403638709369139	0.1196577112526032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1010	0.0	27.29208	1
GCGTCTC	85	1.9754225E-9	23.941177	9
GTATCAA	2930	0.0	23.361774	1
GCGTACT	40	0.0019310185	23.125	17
CGAGCGT	95	7.1413524E-9	21.421053	6
GCTACGA	105	9.822543E-10	21.142857	2
GCTACCG	65	6.901025E-5	19.923077	7
ACGAGCG	105	2.2586391E-8	19.38095	5
TTAACGG	195	0.0	18.97436	35
GGCTACG	120	5.171387E-9	18.5	1
TACCGGC	50	0.0070342813	18.499998	7
TAGGACC	235	0.0	18.106384	4
GCCGGCA	145	1.546141E-10	17.86207	15
CCGTCTA	125	8.574716E-9	17.76	26
AGGACCG	115	6.402479E-8	17.695652	5
AGGTTAC	150	2.5102054E-10	17.266666	7
CGCCGTT	75	2.0669396E-4	17.266666	25
AACGGCC	215	0.0	17.209303	37
TTCGCCG	140	1.8681021E-9	17.178572	24
CGTCTCC	145	2.9776857E-9	16.586208	10
>>END_MODULE