FastQCFastQC Report
Fri 10 Feb 2017
ERR1633148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768110
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33010.4297561547174233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.2963117261850516No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14360.1869523896316934No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13220.17211076538516618No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13020.16950697165770526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12600.16403900483003736No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12160.15831065862962337No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12090.15739933082501203No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11160.1452916899923188No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9540.12420096079988542No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9230.12016508052232103No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8610.1120933199671922No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8430.10974990561247737No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8380.10909895718061215No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8330.10844800874874691No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8270.10766687063050864No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8270.10766687063050864No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8240.10727630157138951No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7880.10258947286195988No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7750.10089700693911027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.027.751
TGCGGTA451.3226536E-424.66666836
GTATCAA20350.023.4545461
TTGCGGT502.7009708E-422.235
CGAGTCG603.724214E-521.58333421
CGTCCGC603.724214E-521.58333429
CGTAGGC705.098289E-621.14285718
AGGCTCG555.1409577E-420.18181824
TCGCCGT656.898319E-519.92307725
ACGTTAG656.898319E-519.92307724
CGCTCTC656.898319E-519.92307729
CTTGGAC1306.9485395E-1018.53
GGCGCGT500.00703280818.531
AGTCGCC701.2185295E-418.523
CATAATA2050.018.048782
AACGGCC3200.017.92187537
TAATATA2950.017.5593224
TATTAGA3600.017.4722232
ATTAGAG3500.017.4428583
CCGCTCT852.7212121E-517.41176428
Produced by FastQC (version 0.11.5)