FastQCFastQC Report
Fri 10 Feb 2017
ERR1633147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620599
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29090.46874068440329425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.2869808040296552No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12510.2015794417973603No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12490.20125717250591765No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11850.19094455517975376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10910.17579789848195052No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10310.16612981973867183No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10080.16242372288708168No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8510.1371255835088358No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7960.1282631779941637No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7620.12278460003963912No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7370.11875623389660635No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7290.11746715673083584No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7230.11650034885650798No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7220.11633921421078668No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7000.11279425200491783No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6990.11263311735919652No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6740.10860475121616375No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6460.10409298113596703No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6220.10022574963865556No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6220.10022574963865556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.028.3467751
CGCCCTT358.8647765E-426.42857226
GCGCAGT451.3222542E-424.66666637
ACGGGTT400.001930190823.1250025
CGGCTAA400.001930190823.12500236
GTATCAA16600.022.9578321
CGCCGTC450.00382385520.55555526
GCCGTCC450.00382385520.55555527
TAAAGGC656.89559E-519.9230774
TGCGGTA951.6730155E-719.47368436
GTCGCCC2950.019.44067837
CGCGCGA902.1489705E-618.532
CGCGATA801.6147173E-518.534
ATAGAAC609.230288E-418.53
TAAGGAC609.230288E-418.53
TCGCCGT500.00703132518.49999825
CGAGTCG500.00703132518.49999821
TTAACGG2500.017.7635
AGGTCGC3250.017.64615435
TGACGCA953.6009787E-617.52631628
Produced by FastQC (version 0.11.5)